Pcr-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores

Applied and Environmental Microbiology

Volumn 75, Issue 9, 2009, Pages 2936-2939

  Rawsthorne, H ^a   Dock, C N ^a,b   Jaykus, L A ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS SUBTILIS SPORES; BACTERIAL ENDOSPORES; INTERCALATING DYE; MOLECULAR METHODS; PROCESS VALIDATION; PROPIDIUM;

NUCLEIC ACIDS;

BACTERIOLOGY;

DNA; INTERCALATING AGENT; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG; AZIDE; DRUG DERIVATIVE; FLUORESCENT DYE; PROPIDIUM IODIDE;

BACTERIUM; DNA; DYE; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; SPORE; VIABILITY;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL ENDOSPORE; BACTERIAL VIABILITY; BACTERIUM CULTURE; EVALUATION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; VALIDATION PROCESS; BACTERIAL SPORE; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL VIABILITY; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

AZIDES; BACILLUS SUBTILIS; DNA; FLUORESCENT DYES; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION; PROPIDIUM; SPORES;

EID: 65549128538     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02524-08     Document Type: Article

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