Detection of Naegleria spp. and Naegleria fowleri: A comparison of flagellation tests, ELISA and PCR

Water Science and Technology

Volumn 47, Issue 3, 2003, Pages 117-122

  Behets, Jonas ^a   Seghi, S ^a   Declerck, P ^a   Verelst, L ^b   Duvivier, L ^b   Van Damme, A ^c   Ollevier, F ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b LABORELEC   (Belgium)

^c Electrabel   (Belgium)

Author keywords

ELISA; Flagellation test; N. fowleri; Naegleria spp.; PCR

Indexed keywords

GENES; MICROORGANISMS; PATHOLOGY;

FLAGELLATION;

DISEASES;

DETECTION METHOD; MICROBIAL POLLUTANT; WATER QUALITY;

ASSAY; BACTERIAL FLAGELLUM; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL HETEROGENEITY; COMPARATIVE STUDY; CONFERENCE PAPER; CONTROLLED STUDY; DNA ISOLATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENOTYPE; MENINGOENCEPHALITIS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGICAL PARAMETERS; NAEGLERIA; NAEGLERIA FOWLERI; NONHUMAN; PARASITE EXAMINATION; PARASITE IDENTIFICATION; PATHOGENESIS; POLYMERASE CHAIN REACTION; SCREENING; STANDARD; WATER CONTAMINATION;

BACTERIA (MICROORGANISMS); NAEGLERIA; NAEGLERIA FOWLERI; PROTOZOA;

EID: 0037217735     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2003.0177     Document Type: Conference Paper

References (8)

1. Cabanes, P.A., Wallet, F., Pringuez, E. and Pernin, P. (2001). Assessing the risk of primary amoebic meningoencephalitis from swimming in the presence of environmental Naegleria fowleri. Appl. Environ. Microbiol., 67(7),2927-2931.
2. Carter, R.F. (1968). Primary meningo-encephalitis: Clinical, pathological and epidemiological features of six fatal cases. J. Pathol. Bacteriol., 96, 1-25.
3. De Jonckheere, J.F., Brown, S., Dobson, P.J., Robinson, B.S. and Pernin, P. (2001). The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria - Description of three new Naegleria spp. Protist, 152, 115-121.
4. Kwaik, Y.A., Gao, L.Y., Stone, B.J., Ventakaraman, C. and Harb, O.S. (1998). Invasion of protozoa by Legionella pneumophila and its role in bacterial ecology and pathogenesis. Appl. Environ. Microbiol., 64, 3127-3133.
5. Marciano-Cabral, F. (1988). Biology of Naegleria spp. Microbiol. Rev., 52, 114-133.
6. Page, F.C. (1988). A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, UK, 122 pp.
7. Pélandakis, M., Serre, S. and Pernin, P. (2000). Analysis of the 5.8S rRNA gene and the internal transcribed spacers in Naegleria spp and in N. fowleri. J. Eukaryot. Microbiol., 47(2), 116-121.
8. Seghi, F. (1999). Guide Pratique Pour le Prélèvement et la Détection de Naegleria fowleri. Laborelec, ENV-104-99-0003/R-FS-fs.