Effect of Mucuna pruriens (Linn.) on oxidative stress-induced structural alteration of Corpus cavernosum in streptozotocin-induced diabetic rat

Journal of Sexual Medicine

Volumn 8, Issue 7, 2011, Pages 1943-1956

  Suresh, Sekar ^a   Prakash, Seppan ^a  

^a UNIVERSITY OF MADRAS   (India)

Author keywords

Corpus cavernosum; Diabetes; Erectile dysfunction; Mucuna pruriens; Oxidative stress; Sexual medicine; Traditional medicine

Indexed keywords

AGENTS ACTING ON THE GENITAL SYSTEM; ANTIOXIDANT; COLLAGEN; GLUTATHIONE; HERBACEOUS AGENT; MUCUNA PRURIENS SEED ETHANOL EXTRACT; NITRATE; NITRIC OXIDE; NITRITE; PLACEBO; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SILDENAFIL; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CORPUS CAVERNOSUM; DIABETES INDUCED ERECTILE DYSFUNCTION; DRUG EFFECT; ENZYME ACTIVITY; ERECTILE DYSFUNCTION; FIBROSIS; LIPID PEROXIDATION; MALE; MORPHOMETRICS; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT; STREPTOZOCIN DIABETES; TREATMENT RESPONSE; VELVET BEAN;

EID: 79960447348     PISSN: 17436095     EISSN: 17436109     Source Type: Journal    
DOI: 10.1111/j.1743-6109.2011.02221.x     Document Type: Article

References (63)

1. Escrig A, Marin R, Abreu P, Gonzalez-Mora JL, Mas M. Changes in mating behavior, erectile function and nitric oxide levels in penile corpora cavernosa in streptozotocin-diabetic rats. Biol Reprod 2002;66:185-9.
2. Angulo J, Cuevas P, Fernández A, Gabancho S, Allona A, Martín-Morales A, Moncada I, Videla S, Sáenz de Tejada I. Diabetes impairs endothelium-dependent relaxation of human penile vascular tissues mediated by NO and EDHF. Biochem Biophys Res Commun 2003;312:1202-8.
3. Jesmin S, Sakuma I, Salah-eldin A, Nonomura K, Hattori Y, Kitabatake A. Diminished penile expression of vascular endothelial growth factor and its receptors at the insulin-resistant stage of a type II diabetic rat model: A possible cause for erectile dysfunction in diabetes. J Mol Endocrinol 2003;31:401-18.
4. De Angelis L, Martella MA, Siniscalchi M, Marino L, Nappo F, Giugliano F, De Lucia D, Giugliano D. Erectile and endothelial dysfunction in type II diabetes: A possible link. Diabetologia 2001;44:1155.
5. Rajfer J. Opportunities and challenges in oral therapy. Int J Impot Res 2000;12(suppl 4):S59-61.
6. Melman A, Rehman J. Pathophysiology of erectile dysfunction. Mol Urol 1999;3:87-102.
7. Misra L, Wagner H. Alkaloidal constituents of Mucuna pruriens seeds. Phytochemistry 2004;65:2565-7.
8. Suresh S, Prithiviraj E, Prakash S. Dose- and time-dependent effects of ethanolic extract of Mucuna pruriens Linn. seed on sexual behaviour of normal male rats. J Ethnopharmacol 2009;22:497-501.
9. Suresh S, Prithiviraj E, Prakash S. Effect of Mucuna pruriens on oxidative stress mediated damage in aged rat sperm. Int J Androl 2010;33:22-32.
10. Pant MC, Joshi LD. Identification of pharmacologically active substances in the seeds of Mucuna pruriens (Abs). Proceedings of Annual Conference of Indian Pharmacological Society. Lucknow, India. Indian J Pharmacol 1970;2:24.
11. Sathiyanarayanan L, Arulmozhi S. S1PHCOG REV: Plant review Mucuna pruriens Linn. A comprehensive review. Pharmacognosy Rev 2007;1:157-62.
12. Phytochemical database. Available at: (accessed April 27, 2010).
13. Adebowale YA, Adeyemi A, Oshodi AA. Variability in the physicochemical, nutritional and antinutritional attributes of six Mucuna species. Food Chem 2005;89:37-48.
14. Suresh S, Prakash S. Effect of Mucuna pruriens (Linn.) on sexual behavior and sperm parameters in streptozotocin-induced diabetic male rat. J Sex Med 2010 Apr 26 [Epub ahead of print] doi: 10.1111/j.1743-6109.2010.01831.x.
15. Grover JK, Vats V, Rathi SS, Dawar R. Traditional Indian anti-diabetic plants attenuate progression of renal damage in streptozotocin induced diabetic mice. J Ethnopharmacol 2001;76:233-8.
16. Olfert ED, Cross BM, McWilliam AA. Guide to the care and use of experimental animals. Vol. 1, 2nd edition. Ottawa, Canada: Canadian Council for Animal Care; 1993.
17. Committee for the Purpose of Control and Supervision on Experiments on Animals. Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) guidelines for laboratory animal facility. Indian J Pharmacol 2003;35:257-74.
18. Driver AS, Kodavanti PR, Mundy WR. Age-related changes in reactive oxygen species production in rat brain homogenates. Neurotoxicol Teratol 2000;22:175-81.
19. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin's phenol reagent. J Biol Chem 1951;193:265-76.
20. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
21. Desai I. Vitamin E analysis methods for animal tissue. Methods Enzymol 1984;105:138-43.
22. Omaye ST, Amball JDT, Sauberlich HE. Selected method for the determination of ascorbic acid in animal cells, tissue and fluid. Methods Enzymol 1979;62:1-11.
23. Moron MS, DePierre JW, Manerwik KB. Levels of glutathione and glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim Biophys Acta 1979;582:67-8.
24. Marklund S, Marklund G. Involvement of superoxide anion radical in the auto oxidation of pyrogallol and a constituent assay for superoxide dismutase. Eur J Biochem 1974;47:469-79.
25. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
26. Rotruck JK, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
27. Staal GEJ, Visser J, Veeger C. Purification and properties of glutathione reductase of human erythrocytes. Biochim Biophys Acta 1969;185:39-48.
28. Habig WH, Palst MJ, Jakoby WB. Glutathione-S-transferase. The first enzymatic step in mercapturic formation. J Biol Chem 1973;249:7130-9.
29. Liu S, Kapoor M, Leask A. Rac1 expression by fibroblasts is required for tissue repair in vivo. Am J Pathol 2009;174:1847-56.
30. Bivalacqua TJ, Armstrong JS, Biggerstaff J, Abdel-Mageed AB, Kadowitz PJ, Hellstrom WJG, Champion HC. Gene transfer of extracellular SOD to the penis reduces superoxide anion and improves erectile function in aged rats. Am J Physiol Heart Circ Physiol 2003;284:H1408-21.
31. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneus detection of nitrate and nitrite. Nitric Oxide 2001;5:62-71.
32. Bancroft JD, Gamble M. Theory and practice of histological techiniques. 3rd edition. London/Edinburgh/New York: Churchill Livingstone; 2002:153-208.
33. Elias H, Hyde DM. An elementary introduction to stereology (quantitative microscopy). Am J Anat 1980;159:412-46.
34. Prakash S, Prithiviraj E, Suresh S. Developmental changes of seminiferous tubule in prenatal, postnatal and adult testis of bonnet monkey (Macaca radiata). Anat Histol Embryol 2008;37:19-23.
35. Mori H, Christensen AK. Morphometric analysis of Leydig cells in the normal rat testis. J Cell Biol 1980;84:340-54.
36. Junqueira LCU, Bignolas G, Brentani RR. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. J Histochem 1979;11:447-55.
37. Dorfman VB, Lopez-Costab JJ, Vegaa C, Bayonaac JC, Capanib F, Loidlb CF, Coirini H. Changes of NADPH-diaphorase reactivity in lumbar spinal cord of short-term streptozotocin induced diabetic rats. Brain Res 2004;997:185-93.
38. Zar JH. Biostatistical analysis. Englewood Cliffs, NJ: Prentice-Hall; 1974:620.
39. Shafiei M, Nobakht M, Fattahi M, Kohneh-Shahri L, Mahmoudiana M. Histochemical assessment of nitric oxide synthase activity in aortic endothelial cells of streptozotocin-induced diabetic rats. Pathophysiology 2003;10:63-7.
40. Alp NJ, Mussa S, Khoo J, Cai S, Guzik T, Jefferson A, Goh N, Rockett KA, Channon KM. Tetrahydrobiopterin-dependent preservation of nitric oxide-mediated endothelial function in diabetes by targeted transgenic GTP-cyclohydrolase I overexpression. J Clin Invest 2003;112:725-35.
41. Kossenjans W, Eis A, Sahay R, Brockman D, Myatt L. Role of peroxynitrite in altered fetal-placental vascular reactivity in diabetes or preeclampsia. Am J Physiol Heart Circ Physiol 2000;278:H1311-9.
42. Chen CA, Druhan LJ, Varadharaj S, Chen YR, Zweier JL. Phosphorylation of endothelial nitric-oxide synthase regulates superoxide generation from the enzyme. J Biol Chem 2008;283:27038-47.
43. Vásquez-Vivar J, Kalyanaraman B, Martásek P. The role of tetrahydrobiopterin in superoxide generation from eNOS: Enzymology and physiological implications. Free Radic Res 2003;37:121-7.
44. Vane JR, Botting RM. The role of chemical mediators released by the endothelium in the control of the cardiovascular system. Int J Tissue React 1992;14:55-64.
45. Shimizu S, Ishii M, Miyasaka Y, Wajima T, Negoro T, Hagiwara T, Kiuchi Y. Possible involvement of hydroxyl radical on the stimulation of tetrahydrobiopterin synthesis by hydrogen peroxide and peroxynitrite in vascular endothelial cells. Int J Biochem Cell Biol 2005;37:864-75.
46. Booth G, Stalker TJ, Lefer AM, Scalia R. Mechanisms of amelioration of glucose-induced endothelial dysfunction following inhibition of protein kinase c in vivo. Diabetes 2002;51:1556-64.
47. Ryu JK, Kim DJ, Lee T, Kang YS, Yoon SM, Suh JK. The role of free radical in the pathogenesis of impotence in streptozotocin-induced diabetic rat. Yonsei Med J 2003;44:236-41.
48. Ferrini MG, Kovanecz I, Sanchez S, Vernet D, Davila HH, Rajfer J, Gonzalez-Cadavid NF. Long-term continuous treatment with sildenafil ameliorates aging-related erectile dysfunction and the underlying corporal fibrosis in the rat. Biol Reprod 2007;76:915-23.
49. Beckman JS, Koppenol WH. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and ugly. Am J Physiol 1996;271:C1424-37.
50. Zou MH, Shi C, Cohen RA. High glucose via peroxynitrite causes tyrosine nitration and inactivation of prostacyclin synthase that is associated with thromboxane/prostaglandin H2 receptor-mediated apoptosis and adhesion molecule expression in cultured human aortic endothelial cells. Diabetes 2002;51:198-203.
51. Kaneto H, Fujii J, Suzuki K, Kasai H, Kawamori R, Kamada T, Taniguchi N. DNA cleavage induced by glycation of Cu, Zn-superoxide dismutase. Biochem J 1994;304:219-25.
52. Moore CR, Wang R. Pathophysiology and treatment of diabetic erectile dysfunction. Asian J Androl 2006;8:675-84.
53. Muller FL, Lustgarten MS, Jang Y, Richardson A, Van Remmen H. Trends in oxidative aging theories. Free Radic Biol Med 2007;43:477-503.
54. Rajeshwar Y, Senthil Kumar GP, Gupta M, Mazumder UK. Studies on in vitro antioxidant activities of methanol extracts of Mucuna pruriens (Fabaceae) seeds. Eur Bull Drug Res 2005;13:31-9.
55. Reiter E, Jiang Q, Christen S. Anti-inflammatory properties of α- and γ-tocopherol. Mol Aspects Med 2007;28:668-91.
56. Majumder PK, Banerjee SK, Roy RK, Chatterjee GC. Effect of insulin on the metabolism of L-ascorbic acid in animals. Biochem Pharmacol 1972;22:759-61.
57. Bode AM, Yavarow CR. Enzymatic basis for altered ascorbicacid and dehydroascorbic acid levels in diabetes. Biochem Biophys Res Commun 1993;191:1347-53.
58. Kashiba M, Oka J, Ichikawa R, Kasahara E, Inayama T, Kageyama A, Kageyama H, Osaka T, Umegaki K, Matsumoto A, Ishikawa T, Nishikimi M, Inoue M, Inoue S. Impaired ascorbic acid metabolism in streptozotocin-induced diabetic rats. Free Radic Biol Med 2002;33:1221-30.
59. Cellek S, Foxwell NA, Moncada S. Two phases of nitrergic neuropathy in streptozotocin-induced diabetic rats. Diabetes 2003;52:2353-62.
60. Ozdemirci S, Yildiz F, Utkan T, Ulak G, Çetinaslan B, Erden F, Gacar N. Impaired neurogenic and endothelium-dependent relaxant responses of corpus cavernosum smooth muscle from hyperthyroid rabbits. Eur J Pharmacol 2001;428:105-11.
61. Rogers RS, Graziottin TM, Lin CS, Kan YW, Lue TF. Intracavernosal vascular endothelial growth factor (VEGF) injection and adeno-associated virus-mediated (VEGF) gene therapy prevent and reverse venogenic erectile dysfunction in rats. Int J Impot Res 2003;15:26-37.
62. McVary KY, Podlasek CA, McKenna KE, Wood D. Intrinsic and extrinsic apoptotic pathways are employed in neuropathic and diabetic models of erectile dysfunction. J Sex Med 2006;3:45.
63. Ferrini MG, Kovanecz I, Sanchez S, Vernet D, Umeh C, Rajfer J, Gonzalez-Cadavid NF. Fibrosis and loss of smooth muscle in the corpora cavernosa precede corporal veno-occlusive dysfunction (CVOD) induced by experimental cavernosal nerve damage in the rat. J Sex Med 2009;6:415-28.