Fibrosis and loss of smooth muscle in the corpora cavernosa precede corporal veno-occlusive dysfunction (CVOD) induced by experimental cavernosal nerve damage in the rat

Journal of Sexual Medicine

Volumn 6, Issue 2, 2009, Pages 415-428

  Ferrini, Monica G ^a,b   Kovanecz, Istvan ^b,c   Sanchez, Sandra ^c   Umeh, Chiome ^c   Rajfer, Jacob ^b,c   Gonzalez Cadavid, Nestor F ^a,b,c  

^a CHARLES R DREW UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c HARBOR UCLA MEDICAL CENTER   (United States)

Author keywords

Apoptosis; cGMP; Collagen; Erectile dysfunction; Fibrosis; Inducible nitric oxide synthase; Nerve sparing; Nitric oxide; Penis; Radical prostatectomy; Smooth muscle

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN; CYCLINE; DYE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; INDUCIBLE NITRIC OXIDE SYNTHASE; MASSON TRICHROME; NEURONAL NITRIC OXIDE SYNTHASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BILATERAL CAVERNOSAL NERVE RESECTION; CELL PROLIFERATION; CONTROLLED STUDY; COROPORAL VENOOCCLUSIVE DYSFUNCTION; CORPUS CAVERNOSUM; ENZYME ACTIVITY; INTERMETHOD COMPARISON; MALE; MUSCLE CELL; MYOFIBROSIS; NERVE INJURY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; SHAM PROCEDURE; SMOOTH MUSCLE; SPRAGUE DAWLEY RAT; STAINING; UROLOGIC SURGERY; VEIN OCCLUSION; WESTERN BLOTTING;

EID: 59349118003     PISSN: 17436095     EISSN: 17436109     Source Type: Journal    
DOI: 10.1111/j.1743-6109.2008.01105.x     Document Type: Article

References (43)

1. Levinson AW, Pavlovich CP, Ward NT, Link RE, Mettee LZ, Su LM. Association of surgeon subjective characterization of nerve sparing quality with potency following laparoscopic radical prostatectomy. J Urol 2008;179:1510-4.
2. Madeb R, Golijanin D, Knopf J, Vicente I, Erturk E, Patel HR, Joseph JV. Patient-reported validated functional outcome after extraperitoneal robotic-assisted nerve-sparing radical prostatectomy. JSLS 2007;11:443-8.
3. Ayyathurai R, Manoharan M, Nieder AM, Kava B, Soloway MS. Factors affecting erectile function after radical retropubic prostatectomy: Results from 1,620 consecutive patients. BJU Int 2008;101:833-6.
4. Briganti A, Salonia A, Gallina A, Chun FK, Karakiewicz PI, Graefen M, Huland H, Rigatti P, Montorsi F. Management of erectile dysfunction after radical prostatectomy in 2007. World J Urol 2007;25:143-8.
5. Luo H, Goldstein I, Udelson D. A three-dimensional theoretical model of the relationship between cavernosal expandability and percent cavernosal smooth muscle. J Sex Med 2007;4:644-5, discussion 651-5.
6. McCullough A, Woo K, Telegrafi S, Lepor H. Is sildenafil failure in men after radical retropubic prostatectomy (RRP) due to arterial disease? Penile duplex Doppler findings in 174 men after RRP. Int J Impot Res 2002;14:462-5.
7. Broderick GA. Evidence based assessment of erectile dysfunction. Int J Impot Res 1998;10(2 suppl):S64-73, discussion S77-9.
8. Nikoobakht M, Saraji A, Meysamie A. Preoperative corporal biopsy as a predictor of postoperative results in venoocclusive erectile dysfunction. Urol J 2005;2:160-4.
9. Hu WL, Hu LQ, Li SW, Zheng XM, Tian BC. Expression of transforming growth factor-beta1 in penile tissue from rats with bilateral cavernosal nerve ablation. BJU Int 2004;94:424-8.
10. Kovanecz I, Rambhatla A, Ferrini MG, Vernet D, Sanchez S, Rajfer J, Gonzalez-Cadavid NF. Chronic daily tadalafil prevents the corporal fibrosis and veno-occlusive dysfunction that occurs after cavernosal nerve resection. BJU Int 2008;101:203-10.
11. Kovanecz I, Rambhatla A, Ferrini M, Vernet D, Sanchez S, Rajfer J, Gonzalez-Cadavid NF. Long-term continuous sildenafil treatment ameliorates corporal veno-occlusive dysfunction (CVOD) induced by cavernosal nerve resection in rats. Int J Impot Res 2008;20:202-12.
12. Ferrini MG, Davila HH, Kovanecz I, Sanchez SP, Gonzalez-Cadavid NF, Rajfer J. Vardenafil prevents fibrosis and loss of corporal smooth muscle that occurs after bilateral cavernosal nerve resection in the rat. Urology 2006;68:429-35.
13. Rambhatla A, Kovanecz I, Ferrini M, Gonzalez-Cadavid NF, Rajfer J. Rationale for phosphodiesterase 5 inhibitor use post-radical prostatectomy: Experimental and clinical review. Int J Impot Res 2008;20:30-4.
14. Davila HH, Rajfer J, Gonzalez-Cadavid NF. Corporal veno-occlusive dysfunction in aging rats: Evaluation by cavernosometry and cavernosography. Urology 2004;64:1261-6.
15. Ferrini MG, Davila H, Valente EG, Gonzalez-Cadavid NF, Rajfer J. Aging-related induction of inducible nitric oxide synthase (iNOS) is vasculo-protective in the arterial media. Cardiovasc Res 2004;61:796-805.
16. Mazza ON, Angerosa M, Becher E, Toblli JE. Differences between Candesartan and Hydralazine in the protection of penile structures in spontaneously hypertensive rats. J Sex Med 2006;3:604-11.
17. Magee T, Zeller CB, Ferrini M, Davila H, Vernet D, Burnett AL, Rajfer J, González-Cadavid NFA. Protein inhibitor of NOS (PIN) is expressed in the rat and mouse penile nerves and co-localizes with penile neuronal NOS (PnNOS). Biol Reprod 2002;68:478-88.
18. Nolazco G, Kovanecz I, Vernet D, Gelfand RA, Tsao J, Ferrini MG, Magee T, Rajfer J, Gonzalez-Cadavid NF. Effect of muscle-derived stem cells on the restoration of corpora cavernosa smooth muscle and erectile function in the aged rat. BJU Int 2008;101:1156-64.
19. Artaza JN, Singh R, Ferrini MG, Braga M, Tsao J, Gonzalez-Cadavid NF. Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/ 2 cells without affecting their differentiation into myofibroblasts. J Endocrinol 2008;196:235-49.
20. Cantini LP, Ferrini MG, Vernet D, Magee TR, Qian A, Gelfand RA, Rajfer J, Gonzalez-Cadavid NF. Profibrotic role of myostatin in peyronie's disease. J Sex Med 2008;5:1607-22.
21. Klein LT, Miller MI, Buttyan R, Raffo AJ, Burchard M, Devris G, Cao YC, Olsson C, Shabsigh R. Apoptosis in the rat penis after penile denervation. J Urol 1997;158:626-30.
22. User HM, Hairston JH, Zelner DJ, McKenna KE, McVary KT. Penile weight and cell subtype specific changes in a post-radical prostatectomy model of erectile dysfunction. J Urol 2003;169:1175-9.
23. Lysiak JJ, Yang SK, Klausner AP, Son H, Tuttle JB, Steers WD. Tadalafil increases Akt and extracellular signal-regulated kinase 1/2 activation, and prevents apoptotic cell death in the penis following denervation. J Urol 2008;179:779-85.
24. Podlasek CA, Meroz CL, Tang Y, McKenna KE, McVary KT. Regulation of cavernous nerve injury-induced apoptosis by sonic hedgehog. Biol Reprod 2007;76:19-28.
25. Maggi M, Filippi S, Ledda F, Magini A, Forti G. Erectile dysfunction: From biochemical pharmacology to advances in medical therapy. Eur J Endocrinol 2000;143:143-54.
26. Erusalimsky JD, Moncada S. Nitric oxide and mitochondrial signaling: From physiology to pathophysiology. Arterioscler Thromb Vasc Biol 2007;27:2524-31.
27. SzabóC, Ischiropoulos H, Radi R. Peroxynitrite: Biochemistry, pathophysiology and development of therapeutics. Nat Rev Drug Discov 2007;6:662-80.
28. Choi BM, Pae HO, Jang SI, Kim YM, Chung HT. Nitric oxide as a pro-apoptotic as well as anti-apoptotic modulator. J Biochem Mol Biol 2002;35:116-26.
29. Muhs A, Heublein B, Schletter J, Herrmann A, Rüdiger M, Sturm M, Grust A, Malms J, Schrader J, von der Leyen HE. Preclinical evaluation of inducible nitric oxide synthase lipoplex gene therapy for inhibition of stent-induced vascular neointimal lesion formation. Hum Gene Ther 2003;14:375-83.
30. Cohen MV, Downey JM. Cardioprotection: Spotlight on PKG. Br J Pharmacol 2007;152:833-4.
31. Burley DS, Ferdinandy P, Baxter GF. Cyclic GMP and protein kinase-G in myocardial ischaemia-reperfusion: Opportunities and obstacles for survival signaling. Br J Pharmacol 2007;152:855-69.
32. Li Q, Guo Y, Tan W, Ou Q, Wu WJ, Sturza D, Dawn B, Hunt G, Cui C, Bolli R. Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappa B dependent pathway. Circulation 2007;116:1577-84.
33. Lagoda G, Jin L, Lehrfeld TJ, Liu T, Burnett AL. FK506 and sildenafil promote erectile function recovery after cavernous nerve injury through antioxidative mechanisms. J Sex Med 2007;4:908-16.
34. Lin G, Bella AJ, Lue TF, Lin CS. Brain-derived neurotrophic factor (BDNF) acts primarily via the JAK/STAT pathway to promote neurite growth in the major pelvic ganglion of the rat: Part 2. J Sex Med 2006;3:821-7; discussion 828-9.
35. Rochlitzer S, Nassenstein C, Braun A. The contribution of neurotrophins to the pathogenesis of allergic asthma. Biochem Soc Trans 2006;34:594-9.
36. Groneberg DA, Rabe KF, Fischer A. Novel concepts of neuropeptide-based drug therapy: Vasoactive intestinal polypeptide and its receptors. Eur J Pharmacol 2006;533:182-94.
37. Palin K, Cunningham C, Forse P, Perry VH, Platt N. Systemic inflammation switches the inflammatory cytokine profile in CNS Wallerian degeneration. Neurobiol Dis 2008;30:19-29.
38. Midrio M. The denervated muscle: Facts and hypotheses. A historical review. Eur J Appl Physiol 2006;98:1-21.
39. Jagoe RT, Goldberg AL. What do we really know about the ubiquitin-proteasome pathway in muscle atrophy? Curr Opin Clin Nutr Metab Care 2001;4:183-90.
40. Ferrini MG, Kovanecz I, Sanchez S, Vernet D, Davila HH, Rajfer J, Gonzalez-Cadavid NF. Long-term continuous treatment with sildenafil ameliorates aging-related erectile dysfunction and the underlying corporal fibrosis in the rat. Biol Reprod 2007;76:915-23.
41. Vernet D, Ferrini MG, Valente E, Magee TR, Bou-Gharios G, Rajfer J, Gonzalez-Cadavid NF. Effect of nitric oxide on the differentiation of fibroblasts into myofibroblasts in the Peyronie's fibrotic plaque and in its rat model. Nitric Oxide 2002;7:262-76.
42. Valente EG, Ferrini MG, Vernet D, Qian A, Rajfer J, Gonzalez-Cadavid NF. PDE L-arginine and PDE inhibitors counteract fibrosis in the Peyronie's fibrotic plaque and related fibroblast cultures. Nitric Oxide 2003;9:229-44.
43. Rajfer J, Gore JL, Kaufman J, Gonzalez-Cadavid N. Case report: Avoidance of palpable corporal fibrosis due to priapism with upregulators of nitric oxide. J Sex Med 2006;3:173-6.