Investigating pathogen biology at the level of the proteome

Proteomics

Volumn 11, Issue 15, 2011, Pages 3190-3202

  Cash, Phillip ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Bacteria; Bacterial pathogenesis; Infectious diseases; Microbiology

Indexed keywords

BIOLOGICAL MARKER;

BACTERIAL GROWTH; BACTERIAL INFECTION; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BRUCELLA ABORTUS; CHRONIC GASTRITIS; DUODENUM ULCER; GASTRITIS; HELICOBACTER PYLORI; HOST; IN VITRO STUDY; IN VIVO STUDY; IRON DEFICIENCY ANEMIA; LEGIONELLA PNEUMOPHILA; LEPTOSPIRA INTERROGANS; LISTERIA MONOCYTOGENES; MEDICAL RESEARCH; MYCOBACTERIUM TUBERCULOSIS; MYCOPLASMA PNEUMONIAE; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEOMICS; REVIEW; SALMONELLA TYPHIMURIUM; STOMACH CANCER; STOMACH ULCER;

BACTERIA; BACTERIAL INFECTIONS; BACTERIAL PROTEINS; BIOLOGICAL MARKERS; HOST-PATHOGEN INTERACTIONS; HUMANS; PROTEOMICS;

BACTERIA (MICROORGANISMS);

EID: 79960399949     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201100029     Document Type: Review

References (106)

1. den Bakker, H. C., Cummings, C. A., Ferreira, V., Vatta, P. et al., Comparative genomics of the bacterial genus Listeria: genome evolution is characterized by limited gene acquisition and limited gene loss. BMC Genomics 2010, 11, 688.
2. Serizawa, M., Sekizuka, T., Okutani, A., Banno, S. et al., Genomewide screening for novel genetic variations associated with ciprofloxacin resistance in Bacillus anthracis. Antimicrob. Agents Chemother. 2010, 54, 2787-2792.
3. van, S. W., Willems, R. J., Genome-based insights into the evolution of Enterococci. Clin. Microbiol. Infect. 2010, 16, 527-532.
4. Fournier, P. E., Drancourt, M., Raoult, D., Bacterial genome sequencing and its use in infectious diseases. Lancet Infect. Dis. 2007, 7, 711-723.
5. Kuhner, S., van Noort, V., Betts, M. J., Leo-Macias, A. et al., Proteome organization in a genome-reduced bacterium. Science 2009, 326, 1235-1240.
6. Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K. et al., Complete genome sequence of enterohemorrhagic Escherichia coli O157: H7 and genomic comparison with laboratory strain K-12. DNA Res. 2001, 8, 11-22.
7. Wiles, T. J., Kulesus, R. R., Mulvey, M. A., Origins and virulence mechanisms of uropathogenic Escherichia coli. Exp. Mol. Pathol. 2008, 85, 11-19.
8. Eppinger, M., Baar, C., Raddatz, G., Huson, D. H. et al., Comparative analysis of four Campylobacterales. Nature Reviews. Microbiology 2004, 2, 872-885.
9. Holden, M., Crossman, L., Cerdeno-Tarraga, A., Parkhill, J., Pathogenomics of non-pathogens. Nat. Rev. Microbiol. 2004, 2, 91.
10. Aranday, C. E., Kaveh, D., Nunez-Garcia, J., Hogarth, P. J. et al., Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice. PLoS One 2010, 5, e11319.
11. Kabara, E., Kloss, C. C., Wilson, M., Tempelman, R. J. et al., A large-scale study of differential gene expression in monocyte-derived macrophages infected with several strains of Mycobacterium avium subspecies paratuberculosis. Brief. Funct. Genomics 2010, 9, 220-237.
12. Fukuto, H. S., Svetlanov, A., Palmer, L. E., Karzai, A. W. et al., Global gene expression profiling of Yersinia pestis replicating inside macrophages reveals the roles of a putative stress-induced operon in regulating type III secretion and intracellular cell division. Infect. Immun. 2010, 78, 3700-3715.
13. Janvilisri, T., Scaria, J., Chang, Y. F., Transcriptional profiling of Clostridium difficile and Caco-2 cells during infection. J. Infect. Dis. 2010, 202, 282-290.
14. Hecker, M., Becher, D., Fuchs, S., Engelmann, S., A proteomic view of cell physiology and virulence of Staphylococcus aureus. Int. J. Med. Microbiol. 2010, 300, 76-87.
15. Cash, P., Proteomics in the study of the molecular taxonomy and epidemiology of bacterial pathogens. Electrophoresis 2009, 30, S133-S141.
16. Hansen, E. J., Wilson, R. M., Baseman, J. B., Two-dimensional gel electrophoretic comparison of proteins from virulent and avirulent strains of Mycoplasma pneumoniae. Infect. Immun. 1979, 24, 468-475.
17. Hansen, E. J., Wilson, R. M., Clyde, W. A., Jr., Baseman, J. B., Characterization of hemadsorption-negative mutants of Mycoplasma pneumoniae. Infect. Immun. 1981, 32, 127-136.
18. Sowa, B. A., Kelly, K. A., Ficht, T. A., Adams, L. G., Virulence associated proteins of Brucella abortus identified by paired two-dimensional gel electrophoretic comparisons of virulent, vaccine and LPS deficient strains. Appl. Theor. Electrophor. 1992, 3, 33-40.
19. Thongboonkerd, V., Chiangjong, W., Saetun, P., Sinchaikul, S. et al., Analysis of differential proteomes in pathogenic and non-pathogenic Leptospira: potential pathogenic and virulence factors. Proteomics 2009, 9, 3522-3534.
20. Silva, E., O'Gorman, M., Becker, S., Auer, G. et al., In the eye of the beholder: does the master see the SameSpots as the novice? J. Proteome Res. 2010, 9, 1522-1532.
21. Uwins, C., Deitrich, C., Argo, E., Stewart, E. et al., Growth-induced changes in the proteome of Helicobacter pylori. Electrophoresis 2006, 27, 1136-1146.
22. Mahairas, G. G., Sabo, P. J., Hickey, M. J., Singh, D. C. et al., Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. J. Bacteriol. 1996, 178, 1274-1282.
23. Pym, A. S., Brodin, P., Brosch, R., Huerre, M. et al., Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti. Mol. Microbiol. 2002, 46, 709-717.
24. Tomb, J. F., White, O., Kerlavage, A. R., Clayton, R. A. et al., The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 1997, 388, 539-547.
25. Alm, R. A., Ling, L. S., Moir, D. T., King, B. L. et al., Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 1999, 397, 176-180.
26. Oh, J. D., Kling-Backhed, H., Giannakis, M., Xu, J. et al., The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression. Proc. Natl. Acad. Sci. USA 2006, 103, 9999-10004.
27. Jungblut, P. R., Bumann, D., Haas, G., Zimny-Arndt, U. et al., Comparative proteome analysis of Helicobacter pylori. Mol. Microbiol. 2000, 36, 710-725.
28. Lock, R. A., Cordwell, S. J., Coombs, G. W., Walsh, B. J. et al., Proteome analysis of Helicobacter pylori: major proteins of type strain NCTC 11637. Pathology 2001, 33, 365-374.
29. Cho, M. J., Jeon, B. S., Park, J. W., Jung, T. S. et al., Identifying the major proteome components of Helicobacter pylori strain 26695. Electrophoresis 2002, 23, 1161-1173.
30. Sabarth, N., Lamer, S., Zimmy-Arndt, U., Jungblut, P. R. et al., Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification and two-dimensional gel electrophoresis. J. Biol. Chem. 2002, 31, 27896-27902.
31. Baik, S. C., Kim, K. M., Song, S. M., Kim, D. S. et al., Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Helicobacter pylori strain 26695. J. Bacteriol. 2004, 186, 949-955.
32. Bumann, D., Aksu, S., Wendland, M., Janek, K. et al., Proteome analysis of secreted proteins of the gastric pathogen Helicobacter pylori. Infect. Immun. 2002, 70, 3396-3403.
33. Kim, N., Weeks, D. L., Shin, J. M., Scott, D. R. et al., Proteins released by Helicobacter pylori in vitro. J. Bacteriol. 2002, 184, 6155-6162.
34. Enroth, H., Akerlund, T., Sille, A., Engstrand, L., Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis. Clin. Diag. Lab. Immunol. 2000, 7, 301-306.
35. Park, J. W., Song, J. Y., Lee, S. G., Jun, J. S. et al., Quantitative analysis of representative proteome components and clustering of Helicobacter pylori clinical strains. Helicobacter 2006, 11, 533-543.
36. Franco, A. T., Friedman, D. B., Nagy, T. A., Romero-Gallo, J. et al., Delineation of a carcinogenic Helicobacter pylori proteome. Mol. Cell. Proteomics 2009, 8, 1947-1958.
37. Barabino, A., Helicobacter pylori-related iron deficiency anemia: a review. Helicobacter 2002, 7, 71-75.
38. Park, S. A., Lee, H. W., Hong, M. H., Choi, Y. W. et al., Comparative proteomic analysis of Helicobacter pylori strains associated with iron deficiency anemia. Proteomics 2006, 6, 1319-1328.
39. Carlsohn, E., Nystrom, J., Karlsson, H., Svennerholm, A. M. et al., Characterization of the outer membrane protein profile from disease-related Helicobacter pylori isolates by subcellular fractionation and nano-LC FT-ICR MS analysis. J. Proteome Res. 2006, 5, 3197-3204.
40. Moxon, R., Tang, C., Challenge of investigating biologically relevant functions of virulence factors in bacterial pathogens. [Review] [104 refs]. Philosophical Transactions of the Royal Society of London - Series B: Biological Sciences 2000, 355, 643-656.
41. Hsiao, A., Zhu, J., Genetic tools to study gene expression during bacterial pathogen infection. Adv. Appl. Microbiol. 2009, 67, 297-314.
42. Lombardo, M. J., Michalski, J., Martinez-Wilson, H., Morin, C. et al., An in vivo expression technology screen for Vibrio cholerae genes expressed in human volunteers. Proc. Natl. Acad. Sci. USA 2007, 104, 18229-18234.
43. Antao, E. M., Ewers, C., Gurlebeck, D., Preisinger, R. et al., Signature-tagged mutagenesis in a chicken infection model leads to the identification of a novel avian pathogenic Escherichia coli fimbrial adhesin. PLoS One 2009, 4, e7796.
44. Schmidt, F., Scharf, S. S., Hildebrandt, P., Burian, M. et al., Time resolved quantitative proteome profiling of host-pathogen interactions: the response of Staphylococcus aureus RN1HG to internalisation by human airway epithelial cells. Proteomics 2010, 10, 2801-2811.
45. Slonczewski, J. L., McGee, D. J., Phillips, J., Kirkpatrick, C. et al., pH-dependent protein profiles of Helicobacter pylori analyzed by two-dimensional gels. Helicobacter 2000, 5, 240-247.
46. Birch, R. M., O'Byrne, C., Booth, I. R., Cash, P., Enrichment of Escherichia coli proteins by column chromatography on reactive dye columns. Proteomics 2003, 3, 764-776.
47. Sonck, K. A., Kint, G., Schoofs, G., Vander, W. C. et al., The proteome of Salmonella typhimurium grown under in vivo-mimicking conditions. Proteomics 2009, 9, 565-579.
48. Eshghi, A., Cullen, P. A., Cowen, L., Zuerner, R. L. et al., Global Proteome Analysis of Leptospira interrogans. J. Proteome Res. 2009, 8, 4564-4578.
49. Alteri, C. J., Mobley, H. L., Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine. Infect. Immun. 2007, 75, 2679-2688.
50. Alteri, C. J., Smith, S. N., Mobley, H. L., Fitness of Escherichia coli during urinary tract infection requires gluconeogenesis and the TCA cycle. PLoS Pathog. 2009, 5, e1000448.
51. Abshire, K. Z., Neidhardt, F. C., Analysis of proteins synthesized by Salmonella typhimurium during growth within a host macrophage. J. Bacteriol. 1993, 175, 3734-3743.
52. Hanawa, T., Yamamoto, T., Kamiya, S., Listeria monocytogenes can grow in macrophages without the aid of proteins induced by environmental stresses. Infect. Immun. 1995, 63, 4595-4599.
53. Mattow, J., Siejak, F., Hagens, K., Becher, D. et al., Proteins unique to intraphagosomally grown Mycobacterium tuberculosis. Proteomics 2006, 6, 2485-2494.
54. Lin, J., Ficht, T. A., Protein synthesis in Brucella abortus induced during macrophage infection. Infect. Immun. 1995, 63, 1409-1414.
55. Rafie-Kolpin, M., Essenberg, R. C., Wyckoff, J. H., III, Identification and comparison of macrophage-induced proteins and proteins induced under various stress conditions in Brucella abortus. Infect. Immun. 1996, 64, 5274-5283.
56. Buchmeier, N. A., Heffron, F., Induction of Salmonella stress proteins upon infection of macrophages. Science 1990, 248, 730-732.
57. Kwaik, Y. A., Harb, O. S., Phenotypic modulation by intracellular bacterial pathogens. Electrophoresis 1999, 20, 2248-2258.
58. Abu Kwaik, Y., Gao, L. Y., Harb, O. S., Stone, B. J., Transcriptional regulation of the macrophage-induced gene (gspA) of Legionella pneumophila and phenotypic characterization of a null mutant. Mol. Microbiol. 1997, 24, 629-642.
59. Van de Velde, S., Delaive, E., Dieu, M., Carryn, S. et al., Isolation and 2-D DIGE proteomic analysis of intracellular and extracellular forms of Listeria monocytogenes. Proteomics 2009, 9, 5484-5496.
60. Mehta, P. K., King, C. H., White, E. H., Murtagh, J. J. et al., Comparison of in vitro models for the study of Mycobacterium tuberculosis invasion and intracellular replication. Infect. Immun. 1996, 64, 2673-2679.
61. Sturgill-Koszycki, S., Haddix, P. L., Russell, D. G., The interaction between Mycobacterium and the macrophage analyzed by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis 1997, 18, 2558-2565.
62. Hughes, V., Smith, S., Garcia-Sanchez, A., Sales, J. et al., Proteomic comparison of Mycobacterium avium subspecies paratuberculosis grown in vitro and isolated from clinical cases of ovine paratuberculosis. Microbiology 2007, 153, 196-205.
63. Kruh, N. A., Troudt, J., Izzo, A., Prenni, J. et al., Portrait of a Pathogen: the Mycobacterium tuberculosis proteome in vivo. PLoS One 2010, 5, e13938.
64. Boyce, J. D., Wilkie, I., Harper, M., Paustian, M. L. et al., Genomic scale analysis of Pasteurella multocida gene expression during growth within the natural chicken host. Infect. Immun. 2002, 70, 6871-6879.
65. Boyce, J. D., Cullen, P. A., Nguyen, V., Wilkie, I. et al., Analysis of the Pasteurella multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host. Proteomics 2006, 6, 870-880.
66. Jacques, M., Belanger, M., Diarra, M. S., Dargis, M. et al., Modulation of Pasteurella multocida capsular polysaccharide during growth under iron-restricted conditions and in vivo. Microbiology 1994, 140, 263-270.
67. Ruffolo, C. G., Jost, B. H., Adler, B., Iron-regulated outer membrane proteins of Pasteurella multocida and their role in immunity. Vet. Microbiol. 1998, 59, 123-137.
68. Monahan, A. M., Callanan, J. J., Nally, J. E., Proteomic analysis of Leptospira interrogans shed in urine of chronically infected hosts. Infect. Immun. 2008, 76, 4952-4958.
69. Twine, S. M., Mykytczuk, N. C., Petit, M. D., Shen, H. et al., In vivo proteomic analysis of the intracellular bacterial pathogen, Francisella tularensis, isolated from mouse spleen. Biochem. Biophys. Res. Commun. 2006, 345, 1621-1633.
70. Becker, D., Selbach, M., Rollenhagen, C., Ballmaier, M. et al., Robust Salmonella metabolism limits possibilities for new antimicrobials. Nature 2006, 440, 303-307.
71. Lange, V., Picotti, P., Domon, B., Aebersold, R., Selected reaction monitoring for quantitative proteomics: a tutorial. Mol. Syst. Biol. 2008, 4, 222.
72. Lange, V., Malmstrom, J. A., Didion, J., King, N. L. et al., Targeted quantitative analysis of Streptococcus pyogenes virulence factors by multiple reaction monitoring. Mol. Cell. Proteomics 2008, 7, 1489-1500.
73. Eylert, E., Schar, J., Mertins, S., Stoll, R. et al., Carbon metabolism of Listeria monocytogenes growing inside macrophages. Mol. Microbiol. 2008, 69, 1008-1017.
74. Eisenreich, W., Slaghuis, J., Laupitz, R., Bussemer, J. et al., 13C isotopologue perturbation studies of Listeria monocytogenes carbon metabolism and its modulation by the virulence regulator PrfA. Proc. Natl. Acad. Sci. USA 2006, 103, 2040-2045.
75. Schar, J., Stoll, R., Schauer, K., Loeffler, D. I. et al., Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes. J. Bacteriol. 2010, 192, 1774-1784.
76. Aminov, R. I., A brief history of the antibiotic era: lessons learned and challenges for the future. Fron. Microbiol. 2011, 1, 1-7.
77. McAtee, C. P., Hoffman, P. S., Berg, D. E., Identification of differentially regulated proteins in metronidozole resistant Helicobacter pylori by proteome techniques. Proteomics 2001, 1, 516-521.
78. Al-Haroni, M., Skaug, N., Bakken, V., Cash, P., Proteomic analysis of ampicillin-resistant oral Fusobacterium nucleatum. Oral Microbiol. Immunol. 2008, 23, 36-42.
79. Vurma-Rapp, U., Kayser, F. H., Hadorn, K., Wiederkehr, F., Mechanism of imipenem resistance acquired by three Pseudomonas aeruginosa strains during imipenem therapy. Eur. J. Clin. Microbiol. Infect. Dis. 1990, 9, 580-587.
80. Michea-Hamzehpour, M., Sanchez, J. C., Epp, S. F., Paquet, N. et al., Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins. Enzyme Protein 1993, 47, 1-8.
81. Cash, P., Argo, E., Ford, L., Lawrie, L. et al., A proteomic analysis of erythromycin resistance in Streptococcus pneumoniae. Electrophoresis 1999, 20, 2259-2268.
82. Pieper, R., Gatlin-Bunai, C. L., Mongodin, E. F., Parmar, P. P. et al., Comparative proteomic analysis of Staphylococcus aureus strains with differences in resistance to the cell wall-targeting antibiotic vancomycin. Proteomics 2006, 6, 4246-4258.
83. Bandow, J. E., Brotz, H., Leichert, L. I., Labischinski, H. et al., Proteomic approach to understanding antibiotic action. Antimicrob. Agents Chemother. 2003, 47, 948-955.
84. Freiberg, C., Brotz-Oesterhelt, H., Labischinski, H., The impact of transcriptome and proteome analyses on antibiotic drug discovery. [Review] [57 refs]. Curr. Opin. Microbiol. 2004, 7, 451-459.
85. Singh, V. K., Jayaswal, R. K., Wilkinson, B. J., Cell wall-active antibiotic induced proteins of Staphylococcus aureus identified using a proteomic approach. FEMS Microbiol. Lett. 2001, 199, 79-84.
86. Coldham, N. G., Randall, L. P., Piddock, L. J., Woodward, M. J., Effect of fluoroquinolone exposure on the proteome of Salmonella enterica serovar typhimurium. J. Antimicrob. Chemother. 2006, 58, 1145-1153.
87. Nanduri, B., Lawrence, M. L., Boyle, C. R., Ramkumar, M. et al., Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome. J. Proteome Res. 2006, 5, 572-580.
88. Archakov, A. I., Govorun, V. M., Dubanov, A. V., Ivanov, Y. D. et al., Protein-protein interactions as a target for drugs in proteomics. Proteomics 2003, 3, 380-391.
89. Wang, Y., Cui, T., Zhang, C., Yang, M. et al., Global protein-protein interaction network in the human pathogen Mycobacterium tuberculosis H37Rv. J. Proteome Res. 2010, 9, 6665-6677.
90. Cui, T., Zhang, L., Wang, X., He, Z. G., Uncovering new signaling proteins and potential drug targets through the interactome analysis of Mycobacterium tuberculosis. BMC Genomics 2009, 10, 118.
91. Sette, A., Rappuoli, R., Reverse vaccinology: developing vaccines in the era of genomics. Immunity 2010, 33, 530-541.
92. Walters, M. S., Mobley, H. L., Bacterial proteomics and identification of potential vaccine targets. Exp.. Rev. Proteomics 2010, 7, 181-184.
93. Teixeira-Gomes, A. P., Cloeckaert, A., Bezard, G., Bowden, R. A. et al., Identification and characterization of Brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. Electrophoresis 1997, 18, 1491-1497.
94. Haas, G., Karaali, G., Ebermayer, K., Metzger, W. G. et al., Immunoproteomics of Helicobacter pylori infection and relation to gastric disease. Proteomics 2002, 2, 313-324.
95. Jungblut, P. R., Grabher, G., Stoffler, G., Comprehensive detection of immunorelevant Borrelia garinii antigens by two-dimensional electrophoresis. Electrophoresis 1999, 20, 3611-3622.
96. Thein, M., Sauer, G., Paramasivam, N., Grin, I. et al., Efficient subfractionation of Gram-negative bacteria for proteomics studies. J. Proteome Res. 2010, 9, 6135-6147.
97. Solis, N., Larsen, M. R., Cordwell, S. J., Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false-positive control. Proteomics 2010, 10, 2037-2049.
98. Bumann, D., Jungblut, P. R., Meyer, T. F., Helicobacter pylori vaccine development based on combined subproteome analysis. Proteomics 2004, 4, 2843-2848.
99. Li, H., Ye, M. Z., Peng, B., Wu, H. K. et al., Immunoproteomic identification of polyvalent vaccine candidates from Vibrio parahaemolyticus outer membrane proteins. J. Proteome Res. 2010, 9, 2573-2583.
100. Nielubowicz, G. R., Smith, S. N., Mobley, H. L., Outer membrane antigens of the uropathogen Proteus mirabilis recognized by the humoral response during experimental murine urinary tract infection. Infect. Immun. 2008, 76, 4222-4231.
101. Sabarth, N., Hurwitz, R., Meyer, T. F., Bumann, D., Multiparameter selection of Helicobacter pylori antigens identifies two novel antigens with high protective efficacy. Infect. Immun. 2002, 70, 6499-6503.
102. Johri, A. K., Paoletti, L. C., Glaser, P., Dua, M. et al., Group B Streptococcus: global incidence and vaccine development. Nat. Rev. Microbiol. 2006, 4, 932-942.
103. Li, Y., Zeng, J., Shi, J., Wang, M. et al., A proteome-scale identification of novel antigenic proteins in Mycobacterium tuberculosis toward diagnostic and vaccine development. J. Proteome Res. 2010, 9, 4812-4822.
104. Montor, W. R., Huang, J., Hu, Y., Hainsworth, E. et al., Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays. Infect. Immun. 2009, 77, 4877-4886.
105. Malmstrom, J., Beck, M., Schmidt, A., Lange, V. et al., Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans. Nature 2009, 460, 762-765.
106. Becher, D., Hempel, K., Sievers, S., Zuhlke, D. et al., A proteomic view of an important human pathogen - towards the quantification of the entire Staphylococcus aureus proteome. PLoS One 2009, 4, e8176.