Detection method for quantifying global DNA methylation by fluorescence correlation spectroscopy

Analytical Biochemistry

Volumn 415, Issue 2, 2011, Pages 145-150

  Umezu, Tomohiro ^a   Ohyashiki, Kazuma ^a   Ohyashiki, Junko H ^a  

^a TOKYO MEDICAL UNIVERSITY   (Japan)

Author keywords

Fluorescence correlation spectroscopy (FCS); Global DNA methylation; Methyl CpG binding domain (MBD)

Indexed keywords

ALKYLATION; CELL CULTURE; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; METHYLATION; SPECTROSCOPIC ANALYSIS;

BIOLOGICAL SAMPLES; DETECTION METHODS; DNA METHYLATION; FLUORESCENCE CORRELATION SPECTROSCOPY; HUMAN LEUKEMIA CELLS; METHYL-CPG-BINDING; METHYLATION ANALYSIS; TRANSLATIONAL DIFFUSION COEFFICIENT;

DNA;

5 AZA 2' DEOXYCYTIDINE; AZACITIDINE; METHYL CPG BINDING PROTEIN 2;

ARTICLE; BINDING AFFINITY; CELL CULTURE; CELL STRAIN K 562; CELL STRAIN U937; DNA METHYLATION; FLUORESCENCE CORRELATION SPECTROSCOPY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL;

EID: 79958265351     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.035     Document Type: Article

References (27)

1. R.L. Momparler Cancer epigenetics Oncogene 22 2003 6479 6483 (Pubitemid 37372327)
2. C. Plass Cancer epigenomics Hum. Mol. Genet. 11 2002 2479 2488 (Pubitemid 35174712)
3. A.G. Tsai, H. Lu, S.C. Raghavan, M. Muschen, C.L. Hsieh, and M.R. Lieber Human chromosomal translocations at CpG sites and a theoretical basis for their lineage and stage specificity Cell 135 2008 1130 1142
4. E.E. Cameron, S.B. Baylin, and J.G. Herman p15(INK4B) CpG island methylation in primary acute leukemia is heterogeneous and suggests density as a critical factor for transcriptional silencing Blood 94 1999 2445 2451 (Pubitemid 29467693)
5. P.A. Jones, and P.W. Laird Cancer epigenetics comes of age Nat. Genet. 21 1999 163 167 (Pubitemid 29070360)
6. C.L. Hsieh Dependence of transcriptional repression on CpG methylation density Mol. Cell. Biol. 14 1994 5487 5494
7. P.A. Jones, and S.B. Baylin The fundamental role of epigenetic events in cancer Nat. Rev. Genet. 3 2002 415 428 (Pubitemid 34587077)
8. M. Ehrlich Cancer-linked DNA hypomethylation and its relationship to hypermethylation Curr. Top. Microbiol. Immunol. 310 2006 251 274 (Pubitemid 47400293)
9. E. Ballestar, and A.P. Wolffe Methyl-CpG-binding proteins. Targeting specific gene repression Eur. J. Biochem. 268 2001 1 6 (Pubitemid 32052241)
10. E. Ballestar, M.F. Paz, L. Valle, S. Wei, M.F. Fraga, J. Espada, J.C. Cigudosa, T.H. Huang, and M. Esteller Methyl-CpG binding proteins identify novel sites of epigenetic inactivation in human cancer EMBO J. 22 2003 6335 6345 (Pubitemid 37522590)
11. M. Ehrenberg, E. Cronvall, and R. Rigler Fluorescence of proteins interacting with nucleic acids. Correction for light absorption FEBS Lett. 18 1971 199 203
12. D. Magde, E.L. Elson, and W.W. Webb Fluorescence correlation spectroscopy. II. An experimental realization Biopolymers 13 1974 29 61
13. M. Kinjo, and R. Rigler Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy Nucleic Acids Res. 23 1995 1795 1799
14. K. Kuroki, S. Kobayashi, M. Shiroishi, M. Kajikawa, N. Okamoto, D. Kohda, and K. Maenaka Detection of weak ligand interactions of leukocyte Ig-like receptor B1 by fluorescence correlation spectroscopy J. Immunol. Methods 320 2007 172 176 (Pubitemid 46350877)
15. G. Maertens, J. Vercammen, Z. Debyser, and Y. Engelborghs Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75 FASEB J. 19 2005 1039 1041 (Pubitemid 40827744)
16. M. Eigen, and R. Rigler Sorting single molecules: application to diagnostics and evolutionary biotechnology Proc. Natl. Acad. Sci. USA 91 1994 5740 5747 (Pubitemid 24188218)
17. M. Bannai, K. Higuchi, T. Akesaka, M. Furukawa, M. Yamaoka, K. Sato, and K. Tokunaga Single-nucleotide-polymorphism genotyping for whole-genome-amplified samples using automated fluorescence correlation spectroscopy Anal. Biochem. 327 2004 215 221 (Pubitemid 38402190)
18. H. Nakata, T. Ohtsuki, and M. Sisido A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates Anal. Biochem. 390 2009 121 125
19. R. Abe, K. Shiraga, S. Ebisu, H. Takagi, and T. Hohsaka Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons J. Biosci. Bioeng. 110 2010 32 38
20. J. Berger, and A. Bird Role of MBD2 in gene regulation and tumorigenesis Biochem. Soc. Trans. 33 2005 1537 1540 (Pubitemid 41779733)
21. M. Fodermayr, J. Proll, O. Zach, C. Wechselberger, and D. Lutz In vitro detection of methylated DNA via recombinant protein MBD2b Mol. Biol. Rep. 36 2009 1859 1862
22. O.J. Sansom, J. Berger, S.M. Bishop, B. Hendrich, A. Bird, and A.R. Clarke Deficiency of Mbd2 suppresses intestinal tumorigenesis Nat. Genet. 34 2003 145 147 (Pubitemid 36666922)
23. P.A. Wade Methyl CpG-binding proteins and transcriptional repression Bioessays 23 2001 1131 1137
24. T.A. Rauch, and G.P. Pfeifer The MIRA method for DNA methylation analysis Methods Mol. Biol. 507 2009 65 75
25. M. Karimi, S. Johansson, and T.J. Ekstrom Using LUMA: a luminometric-based assay for global DNA-methylation Epigenetics 1 2006 45 48
26. V. Martin, H.F. Jorgensen, A.S. Chaubert, J. Berger, H. Barr, P. Shaw, A. Bird, and P. Chaubert MBD2-mediated transcriptional repression of the p14ARF tumor suppressor gene in human colon cancer cells Pathobiology 75 2008 281 287
27. J.G. Herman, and S.B. Baylin Gene silencing in cancer in association with promoter hypermethylation N. Engl. J. Med. 349 2003 2042 2054 (Pubitemid 37448928)