In vitro detection of methylated DNA via recombinant protein MBD2b

Molecular Biology Reports

Volumn 36, Issue 7, 2009, Pages 1859-1862

  Födermayr, Mathilde ^a,b   Pröll, Johannes ^a,b   Zach, Otto ^a   Wechselberger, Christian ^b   Lutz, Dieter ^a  

^a ELISABETHINEN HOSPITAL   (Austria)

^b Upper Austrian Research GmbH   (Austria)

Author keywords

5 Methyl cytosine; DNA methylation; Mbd2b; Methyl binding domain proteins

Indexed keywords

CYTOSINE; GENOMIC DNA; GREEN FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; PROTEIN MBD2B; PYROXYLIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; DNA DETERMINATION; DNA METHYLATION; GENE SILENCING; IN VITRO STUDY; NONHUMAN; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN SYNTHESIS; REGULATORY MECHANISM; TRANSCRIPTION REGULATION; TRANSCRIPTION REPRESSION;

ANTIBODIES; DNA METHYLATION; DNA-BINDING PROTEINS; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 68149173369     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-008-9391-4     Document Type: Article

References (10)

1. A Serman M Vlahovic L Serman 2006 DNA methylation as a regulatory mechanism for gene expression in mammals Coll Antropol 30 665 671
2. M Esteller 2006 Epigenetics provides a new generation of oncogenes and tumour-suppressor genes Br J Cancer 94 179 183 10.1038/sj.bjc.6602918 (Pubitemid 43151531)
3. JP Issa 2003 Age-related epigenetic changes and the immune system Clin Immunol 109 103 108 10.1016/S1521-6616(03)00203-1 (Pubitemid 37324747)
4. PA Jones 1996 DNA methylation errors and cancer Cancer Res 56 2463 2467
5. M Gold J Hurwitz M Anders 1963 The enzymatic methylation of RNA and DNA Biochem Biophys Res Commun 11 107 114 10.1016/0006-291X(63)90075-5
6. KD Robertson 2005 DNA methylation and human disease Nat Rev Genet 6 597 610 10.1038/nrg1655 (Pubitemid 41044297)
7. X Nan RR Meehan A Bird 1993 Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2 Nucleic Acids Res 21 4886 4892 10.1093/nar/21.21.4886
8. E Ballestar AP Wolffe 2001 Methyl-CpG-binding proteins. Targeting specific gene repression Eur J Biochem 268 1 6 10.1046/j.1432-1327.2001.01869.x (Pubitemid 32052241)
9. C Grunau SJ Clark A Rosenthal 2001 Bisulfite genomic sequencing: systematic investigation of critical experimental parameters Nucleic Acids Res 29 E65 E5 10.1093/nar/29.13.e65
10. B Hendrich A Bird 1998 Identification and characterization of a family of mammalian methyl-CpG binding proteins Mol Cell Biol 18 6538 6547