Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons

Journal of Bioscience and Bioengineering

Volumn 110, Issue 1, 2010, Pages 32-38

  Abe, Ryoji ^a,b   Shiraga, Kaori ^a,b   Ebisu, Shogo ^b   Takagi, Hiroaki ^b   Hohsaka, Takahiro ^a  

^a JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^b ProteinExpress Co Ltd   (Japan)

Author keywords

Cell free translation; Fluorescence labeling; Four base codon; Non natural amino acid; Protein engineering

Indexed keywords

AMINO ACID INCORPORATION; ANTICODONS; CELL-FREE; ESCHERICHIA COLI CELLS; EXPRESSION LEVELS; FLUORESCENCE LABELING; FLUORESCENT LABELS; FRAMESHIFT; IN-CELL; LABELED PROTEINS; N-TERMINALS; NON NATURAL AMINO ACIDS; PHOTOSTABLE; RHODAMINE DYES; VISIBLE WAVELENGTHS;

BIOCHEMICAL ENGINEERING; ESCHERICHIA COLI; FLUORESCENCE; GENETIC ENGINEERING; LABELING; ORGANIC ACIDS; PEPTIDES; SYNTHESIS (CHEMICAL);

AMINO ACIDS;

AMINO ACID; TETRAMETHYLRHODAMINE; TRANSFER RNA;

ACCURACY; AMINO ACID METABOLISM; AMINO TERMINAL SEQUENCE; ANTICODON; ARTICLE; CHEMICAL LABELING; CONTROLLED STUDY; FLUORESCENCE; GENE INSERTION; NONHUMAN; POSITION EFFECT; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; RIBOSOMAL FRAMESHIFTING; RNA TRANSLATION; STOP CODON;

AMINO ACIDS; CELL-FREE SYSTEM; CODON; ESCHERICHIA COLI; FLUORESCENT DYES; OLIGOPEPTIDES; PROTEINS; STAINING AND LABELING;

ESCHERICHIA COLI;

EID: 77953123601     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.01.003     Document Type: Article

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