Detection of weak ligand interactions of leukocyte Ig-like receptor B1 by fluorescence correlation spectroscopy

Journal of Immunological Methods

Volumn 320, Issue 1-2, 2007, Pages 172-176

  Kuroki, Kimiko ^a   Kobayashi, Sayoko ^b   Shiroishi, Mitsunori ^a   Kajikawa, Mizuho ^a   Okamoto, Naoaki ^b   Kohda, Daisuke ^a   Maenaka, Katsumi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b OLYMPUS CORPORATION   (Japan)

Author keywords

Cell surface receptor; Fluorescence correlation spectroscopy; Leukocyte Ig like receptor; MHC class I; Protein protein interaction; Weak interactions

Indexed keywords

CELL RECEPTOR; CELL SURFACE RECEPTOR; CYSTEINE; LEUKOCYTE IMMUNE GLOBULIN LIKE RECEPTOR B1; LIGAND; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MEMBRANE RECEPTOR; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; DISSOCIATION CONSTANT; FLUORESCENCE CORRELATION SPECTROSCOPY; IMMUNOCOMPETENT CELL; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SURFACE PLASMON RESONANCE;

ANTIGENS, CD; CELLS, CULTURED; HUMANS; LEUKOCYTES; LIGANDS; MAJOR HISTOCOMPATIBILITY COMPLEX; PROTEIN BINDING; RECEPTORS, IMMUNOLOGIC; SPECTROMETRY, FLUORESCENCE;

EID: 33847637989     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.11.009     Document Type: Article

References (13)

1. Borsch M., Turina P., Eggeling C., Fries J.R., Seidel C.A., Labahn A., and Graber P. Conformational changes of the H+-ATPase from Escherichia coli upon nucleotide binding detected by single molecule fluorescence. FEBS Lett. 437 (1998) 251
2. Colonna M., Navarro F., Bellon T., Llano M., Garcia P., Samaridis J., Angman L., Cella M., and Lopez-Botet M. A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells. J. Exp. Med. 186 (1997) 1809
3. Eggeling C., Fries J.R., Brand L., Gunther R., and Seidel C.A. Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 1556
4. Kinjo M., and Rigler R. Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy. Nucleic Acids Res. 23 (1995) 1795
5. Kinjo M., Nishimura G., Koyama T., Mets R., and Rigler. Single-molecule analysis of restriction DNA fragments using fluorescence correlation spectroscopy. Anal. Biochem. 260 (1998) 166
6. Kobayashi T., Okamoto N., Sawasaki T., and Endo Y. Detection of protein-DNA interactions in crude cellular extracts by fluorescence correlation spectroscopy. Anal. Biochem. 332 (2004) 58
7. Kuroki K., Tsuchiya N., Shiroishi M., Rasubala L., Yamashita Y., Matsuta K., Fukazawa T., Kusaoi M., Murakami Y., Takiguchi M., Juji T., Hashimoto H., Kohda D., Maenaka K., and Tokunaga K. Extensive polymorphisms of LILRB1 (ILT2, LIR1) and their association with HLA-DRB1 shared epitope negative rheumatoid arthritis. Hum. Mol. Genet. 14 (2005) 2469
8. Magde D., Elson E.L., and Webb W.W. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 13 (1974) 29
9. Meseth U., Wohland T., Rigler R., and Vogel H. Resolution of fluorescence correlation measurements. Biophys. J. 76 (1999) 1619
10. Saverino D., Fabbi M., Ghiotto F., Merlo A., Bruno S., Zarcone D., Tenca C., Tiso M., Santoro G., Anastasi G., Cosman D., Grossi C.E., and Ciccone E. The CD85/LIR-1/ILT2 inhibitory receptor is expressed by all human T lymphocytes and down-regulates their functions. J. Immunol. 165 (2000) 3742
11. Shiroishi M., Tsumoto K., Amano K., Shirakihara Y., Colonna M., Braud V.M., Allan D.S., Makadzange A., Rowland-Jones S., Willcox B., Jones E.Y., van der Merwe P.A., Kumagai I., and Maenaka K. Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 8856
12. Shiroishi M., Kuroki K., Tsumoto K., Yokota A., Sasaki T., Amano K., Shimojima T., Shirakihara Y., Rasubala L., van der Merwe P.A., Kumagai I., Kohda D., and Maenaka K. Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1 (LILRB1/ILT2/CD85j). J. Mol. Biol. 355 (2006) 237
13. Wolcke J., Reimann M., Klumpp M., Gohler T., Kim E., and Deppert W. Analysis of p53 "latency" and "activation" by fluorescence correlation spectroscopy. Evidence for different modes of high affinity DNA binding. J. Biol. Chem. 278 (2003) 32587