A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 121-125

  Nakata, Hidetaka ^a,b   Ohtsuki, Takashi ^a   Sisido, Masahiko ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b OLYMPUS CORPORATION   (Japan)

Author keywords

Fluorescence correlation spectroscopy; Four base codon; Position specific labeling; Protease assay; Protease substrate

Indexed keywords

FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PROTEINS; SPECTROSCOPIC ANALYSIS;

FLUORESCENCE CORRELATION SPECTROSCOPY; FOUR-BASE CODON; HIGH THROUGHPUT SCREENING; INHIBITORY ACTIVITY; MATRIX METALLOPROTEINASE 9 (MMP 9); PROTEASE ACTIVITIES; PROTEASE ASSAY; SENSITIVE DETECTION;

SUBSTRATES;

CASPASE 3; ELONGATION FACTOR TS; GELATINASE B; MATRIX METALLOPROTEINASE INHIBITOR;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DRUG LABELING; ENZYME ACTIVITY; FLUORESCENCE CORRELATION SPECTROSCOPY; HIGH THROUGHPUT SCREENING; MOLECULAR WEIGHT; PRIORITY JOURNAL; SOLUBILITY;

EID: 65849133747     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.049     Document Type: Article

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