Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: Comparison to bovine spongiform encephalopathy in cattle

Journal of Veterinary Diagnostic Investigation

Volumn 23, Issue 3, 2011, Pages 407-420

  Hamir, Amir N ^a   Kehrli, Marcus E ^a   Kunkle, Robert A ^a   Greenlee, Justin J ^a   Nicholson, Eric M ^a   Richt, Jürgen A ^a   Miller, Janice M ^a   Cutlip, Randall C ^a  

^a NATIONAL ANIMAL DISEASE CENTER   (United States)

Author keywords

Bovine spongiform encephalopathy; Cattle; Chronic wasting disease; Prion diseases; PrP immunohistochemistry; PrP western blot; Spongiform encephalopathy; Transmissible mink encephalopathy; Variant creutzfeldt jakob disease

Indexed keywords

ANIMAL; ANIMAL DISEASE; ARTICLE; BOVINE SPONGIFORM ENCEPHALOPATHY; CATTLE; CATTLE DISEASE; CHRONIC WASTING DISEASE; COMPARATIVE STUDY; CREUTZFELDT JAKOB DISEASE; DEER; DISEASE PREDISPOSITION; DISEASE TRANSMISSION; GOAT; GOAT DISEASE; HUMAN; PATHOGENICITY; PRION; PRION DISEASE; SCRAPIE; SHEEP; SHEEP DISEASE;

ANIMALS; CATTLE; CATTLE DISEASES; CREUTZFELDT-JAKOB SYNDROME; DEER; DISEASE SUSCEPTIBILITY; ENCEPHALOPATHY, BOVINE SPONGIFORM; GOAT DISEASES; GOATS; HUMANS; PRION DISEASES; PRIONS; SCRAPIE; SHEEP; SHEEP DISEASES; WASTING DISEASE, CHRONIC;

ANIMALIA; BOS; BOVINAE; CAPRA HIRCUS; CERVIDAE; MUSTELA; OVIS ARIES;

EID: 79957725070     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/1040638711403404     Document Type: Article

References (119)

1. Arnold ME, Wilesmith JW: 2004, Estimation of the age-dependent risk of infection to BSE of dairy cattle in Great Britain. Prev Vet Med 66:35-47.
2. Austbo L, Espenes A, Olsaker I, et al.: 2006, Lymphoid follicles of the ileal Peyer's patch of lambs express low levels of PrP, as demonstrated by quantitative real-time RT-PCR on microdissected tissue compartments, in situ hybridization and immunohistochemistry. J Gen Virol 87: 3463-3471.
3. Baeten LA, Powers BE, Jewell JE, et al.: 2007, A natural case of chronic wasting disease in a free-ranging moose (Alces alces shirasi). J Wildl Dis 43:309-314.
4. Sc) into contiguous membranes. EMBO J 21:1031-1040.
5. Baron T, Bencsik A, Biacabe AG, et al.: 2007, Phenotypic similarity of transmissible mink encephalopathy in cattle and L-type bovine spongiform encephalopathy in a mouse model. Emerg Infect Dis 13:1887-1894.
6. Baron T, Biacabe AG, Arsac JN, et al.: 2007, Atypical transmissible spongiform encephalopathies (TSEs) in ruminants. Vaccine 25:5625-5630.
7. Baron TG, Biacabe AG, Bencsik A, Langeveld JP: 2006, Transmission of new bovine prion to mice. Emerg Infect Dis 12:1125-1128.
8. Benestad SL, Sarradin P, Thu B, et al.: 2003, Cases of scra-pie with unusual features in Norway and designation of a new type, Nor98. Vet Rec 153:202-208.
9. Beringue V, Andreoletti O, Le Dur A, et al.: 2007, A bovine prion acquires an epidemic bovine spongiform encepha-lopathy strain-like phenotype on interspecies transmission. J Neurosci 27:6965-6971.
10. Beringue V, Bencsik A, Le Dur A, et al.: 2006, Isolation from cattle of a prion strain distinct from that causing bovine spon-giform encephalopathy. PLoS Pathog 2:e112.
11. Biacabe AG, Laplanche JL, Ryder S, Baron T: 2004, Distinct molecular phenotypes in bovine prion diseases. EMBO Rep 5:110-115.
12. Bradley R: 1996, Experimental transmission of bovine spon-giform encephalopathy. In: Transmissible subacute spongi-form encephalopathies: prion diseases, ed. Court L, Dodet B, pp. 51-56. Elsevier, Amsterdam, The Netherlands.
13. Bradley R: 1999, BSE transmission studies with particular reference to blood. Dev Biol Stand 99:35-40.
14. Brown P, Cervenakova L: 2005, A prion lexicon (out of control). Lancet 365:122.
15. Brunelle BW, Hamir AN, Baron T, et al.: 2007, Polymorphisms of the prion gene promoter region that influence classical bovine spongiform encephalopathy susceptibility are not applicable to other transmissible spongiform encephalopathies in cattle. J Anim Sci 85:3142-3147.
16. Buschmann A, Gretzschel A, Biacabe AG, et al.: 2006, Atypical BSE in Germany-proof of transmissibility and biochemical characterization. Vet Microbiol 117:103-116.
17. Buschmann A, Groschup MH: 2005, Highly bovine spongi-form encephalopathy-sensitive transgenic mice confirm the essential restriction of infectivity to the nervous system in clinically diseased cattle. J Infect Dis 192:934-942.
18. Capobianco R, Casalone C, Suardi S, et al.: 2007, Conversion of the BASE prion strain into the BSE strain: the origin of BSE? PLoS Pathog 3:e31.
19. Casalone C, Caramelli M, Crescio MI, et al.: 2006, BSE immunohistochemical patterns in the brainstem: a comparison between UK and Italian cases. Acta Neuropathol 111: 444-449.
20. Casalone C, Zanusso G, Acutis P, et al.: 2004, Identification of a second bovine amyloidotic spongiform encephalopathy: molecular similarities with sporadic Creutzfeldt-Jakob disease. Proc Natl Acad Sci USA 101:3065-3070.
21. Caughey B, Baron GS: 2002, Factors affecting interactions between prion protein isoforms. Biochem Soc Trans 30: 565-569.
22. Clark WW, Hourrigan JL, Hadlow WJ: 1995, Encephalopathy in cattle experimentally infected with the scrapie agent. Am J Vet Res 56:606-612.
23. Comoy EE, Casalone C, Lescoutra-Etchegaray N, et al.: 2008, Atypical BSE (BASE) transmitted from asymptomatic aging cattle to a primate. PLoS ONE 3:e3017.
24. Cutlip RC, Miller JM, Hamir AN, et al.: 2001, Resistance of cattle to scrapie by the oral route. Can J Vet Res 65: 131-132.
25. Cutlip RC, Miller JM, Lehmkuhl HD: 1997, Second passage of a US scrapie agent in cattle. J Comp Pathol 117: 271-275.
26. Cutlip RC, Miller JM, Race RE, et al.: 1994, Intracerebral transmission of scrapie to cattle. J Infect Dis 169:814-820.
27. De Bosschere H, Roels S, Vanopdenbosch E: 2004, Atypical case of bovine spongiform encephalopathy in an east-Flemish cow in Belgium. Int J Appl Res Vet Med 2:52-55.
28. Dudas S, Yang J, Graham C, et al.: 2010, Molecular, biochemical and genetic characteristics of BSE in Canada. PLoS ONE 5:e10638.
29. Eckroade RJ, Zu Rhein GM, Hanson RP: 1973, Transmissible mink encephalopathy in carnivores: clinical, light and electron microscopic studies in raccoons, skunks and ferrets. J Wildl Dis 9:229-240.
30. Eckroade RJ, Zu Rhein GM, Marsh RF, Hanson RP: 1970, Transmissible mink encephalopathy: experimental transmission to the squirrel monkey. Science 169:1088-1090.
31. Edgeworth JA, Gros N, Alden J, et al.: 2010, Spontaneous generation of mammalian prions. Proc Natl Acad Sci U S A 107:14402-14406.
32. Sc propagation in experimental and natural scrapie in sheep with different PrP genotypes. Vet Pathol 42:258-274.
33. Sc in rectal biopsy and necropsy samples from sheep with experimental scrapie. J Comp Pathol 134:115-125.
34. Espinosa JC, Morales M, Castilla J, et al.: 2007, Progression of prion infectivity in asymptomatic cattle after oral bovine spongiform encephalopathy challenge. J Gen Virol 88: 1379-1383.
35. Fediaevsky A, Gasqui P, Calavas D, Ducrot C: 2010, Discrepant epidemiological patterns between classical and atypical scrapie in sheep flocks under French TSE control measures. Vet J 185:338-340.
36. Greenlee JJ, Hamir AN, West Greenlee MH: 2006, Abnormal prion accumulation associated with retinal pathology in experimentally inoculated scrapie-affected sheep. Vet Pathol 43:733-739.
37. Hamir AN, Cutlip RC, Miller JM, et al.: 2001, Preliminary findings on the experimental transmission of chronic wasting disease agent of mule deer to cattle. J Vet Diagn Invest 13:91-96.
38. Hamir AN, Kunkle RA, Cutlip RC, et al.: 2005, Experimental transmission of chronic wasting disease agent from mule deer to cattle by the intracerebral route. J Vet Diagn Invest 17:276-281.
39. mule deer) agent to cattle. J Comp Pathol 134:63-69.
40. Hamir AN, Kunkle RA, Miller JM, et al.: 2006, First and second cattle passage of transmissible mink encephalopathy by intracerebral inoculation. Vet Pathol 43:118-126.
41. Hamir AN, Kunkle RA, Miller JM, Hall SM: 2006, Abnormal prion protein in ectopic lymphoid tissue in a kidney of an asymptomatic white-tailed deer experimentally inoculated with the agent of chronic wasting disease. Vet Pathol 43: 367-369.
42. res) by immunohistochemistry in striated muscle tissues of animals experimentally inoculated with agents of transmissible spongiform encephalopathy. Vet Pathol 41: 78-81.
43. Hamir AN, Miller JM, Cutlip RC, et al.: 2003, Preliminary observations on the experimental transmission of scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation. Vet Pathol 40:81-85.
44. Hamir AN, Miller JM, Cutlip RC, et al.: 2004, Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracere-bral inoculation: final outcome of the experiment. J Vet Diagn Invest 16:316-321.
45. Hamir AN, Miller JM, Kunkle RA, et al.: 2007, Susceptibility of cattle to first-passage intracerebral inoculation with chronic wasting disease agent from white-tailed deer. Vet Pathol 44:487-493.
46. Heggebo R, Gonzalez L, Press CM, et al.: 2003, Disease-associated PrP in the enteric nervous system of scrapie-affected Suffolk sheep. J Gen Virol 84:1327-1338.
47. Heggebo R, Press CM, Gunnes G, et al.: 2000, Distribution of prion protein in the ileal Peyer's patch of scrapie-free lambs and lambs naturally and experimentally exposed to the scrapie agent. J Gen Virol 81:2327-2337.
48. Heggebo R, Press CM, Gunnes G, et al.: 2002, Distribution and accumulation of PrP in gut-associated and peripheral lym-phoid tissue of scrapie-affected Suffolk sheep. J Gen Virol 83:479-489.
49. Heggebo R, Press CM, Gunnes G, et al.: 2003, Detection of PrPSc in lymphoid tissues of lambs experimentally exposed to the scrapie agent. J Comp Pathol 128:172-181.
50. Hopp P, Omer MK, Heier BT: 2006, A case-control study of scrapie Nor98 in Norwegian sheep flocks. J Gen Virol 87: 3729-3736.
51. Houston EF, Gravenor MB: 2003, Clinical signs in sheep experimentally infected with scrapie and BSE. Vet Rec 152: 333-334.
52. Houston F, Foster JD, Chong A, et al.: 2000, Transmission of BSE by blood transfusion in sheep. Lancet 356:999-1000.
53. Houston F, McCutcheon S, Goldmann W, et al.: 2008, Prion diseases are efficiently transmitted by blood transfusion in sheep. Blood 112:4739-4745.
54. Hunter N, Foster J, Chong A, et al.: 2002, Transmission of prion diseases by blood transfusion. J Gen Virol 83: 2897-2905.
55. Jacobs JG, Langeveld JP, Biacabe AG, et al.: 2007, Molecular discrimination of atypical bovine spongiform encephalopathy strains from a geographical region spanning a wide area in Europe. J Clin Microbiol 45:1821-1829.
56. Jeffrey M, Gonzalez L: 2004, Pathology and pathogenesis of bovine spongiform encephalopathy and scrapie. Curr Top Microbiol Immunol 284:65-97.
57. Jeffrey M, Gonzalez L: 2007, Classical sheep transmissible spongiform encephalopathies: pathogenesis, pathological phenotypes and clinical disease. Neuropathol Appl Neurobiol 33:373-394.
58. Jeffrey M, Gonzalez L, Espenes A, et al.: 2006, Transportation of prion protein across the intestinal mucosa of scra-pie-susceptible and scrapie-resistant sheep. J Pathol 209: 4-14.
59. Jeffrey M, Martin S, Gonzalez L, et al.: 2001, Differential diagnosis of infections with the bovine spongiform enceph-alopathy (BSE) and scrapie agents in sheep. J Comp Pathol 125:271-284.
60. Jeffrey M, McGovern G, Martin S, et al.: 2000, Cellular and sub-cellular localisation of PrP in the lymphoreticular system of mice and sheep. Arch Virol Suppl 16:23-38.
61. Kimberlin RH: 1992, Bovine spongiform encephalopathy. Rev Sci Tech 11:347-489.
62. Kimberlin RH: 1993, Bovine spongiform encephalopathy: an appraisal of the current epidemic in the United Kingdom. Intervirology 35:208-218.
63. Kong Q, Zheng M, Casalone C, et al.: 2008, Evaluation of the human transmission risk of an atypical bovine spongiform encephalopathy prion strain. J Virol 82:3697-3701.
64. Konold T, Lee YH, Stack MJ, et al.: 2006, Different prion disease phenotypes result from inoculation of cattle with two temporally separated sources of sheep scrapie from Great Britain. BMC Vet Res 2:31.
65. Lauren J, Gimbel DA, Nygaard HB, et al.: 2009, Cellular prion protein mediates impairment of synaptic plasticity by amyloid-beta oligomers. Nature 457:1128-1132.
66. Le Dur A, Beringue V, Andreoletti O, et al.: 2005, A newly identified type of scrapie agent can naturally infect sheep with resistant PrP genotypes. Proc Natl Acad Sci U S A 102: 16031-16036.
67. Legname G, Baskakov IV, Nguyen HO, et al.: 2004, Synthetic mammalian prions. Science 305:673-676.
68. Lombardi G, Casalone C, D' Angelo A, et al.: 2008, Intra-species transmission of BASE induces clinical dullness and amyotrophic changes. PLoS Pathog 4:e1000075.
69. Marsh RF: 1972, Animal model of human disease: Kuru, Creutzfeldt-Jakob disease (slow virus infections). Animal model: transmissible mink encephalopathy, scrapie-like disease of mink. Am J Pathol 69:209-212.
70. Marsh RF, Bessen RA, Lehmann S, Hartsough GR: 1991, Epidemiological and experimental studies on a new incident of transmissible mink encephalopathy. J Gen Virol 72(pt 3): 589-594.
71. Marsh RF, Burger D, Eckroade R, et al.: 1969, A preliminary report on the experimental host range of the transmissible mink encephalopathy agent. J Infect Dis 120:713-719.
72. Martin S, Jeffrey M, Gonzalez L, et al.: 2009, Immunohisto-chemical and biochemical characteristics of BSE and CWD in experimentally infected European red deer (Cervus elaphus elaphus). BMC Vet Res 5:26.
73. Mathiason CK, Powers JG, Dahmes SJ, et al.: 2006, Infectious prions in the saliva and blood of deer with chronic wasting disease. Science 314:133-136.
74. Miller MW, Hobbs NT, Tavener SJ: 2006, Dynamics of prion disease transmission in mule deer. Ecol Appl 16:2208-2214.
75. Miller MW, Wild MA, Williams ES: 1998, Epidemiology of chronic wasting disease in captive Rocky Mountain elk. J Wildl Dis 34:532-538.
76. Miller MW, Williams ES, Hobbs NT, Wolfe LL: 2004, Environmental sources of prion transmission in mule deer. Emerg Infect Dis 10:1003-1006.
77. Nicholson EM, Brunelle BW, Richt JA, et al.: 2008, Identification of a heritable polymorphism in bovine PRNP associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE. PLoS ONE 3:e2912.
78. Peden AH, Ritchie DL, Head MW, Ironside JW: 2006, Detection and localization of PrPSc in the skeletal muscle of patients with variant, iatrogenic, and sporadic forms of Creutzfeldt-Jakob disease. Am J Pathol 168:927-935.
79. Polak MP, Zmudzinski JF, Jacobs JG, Langeveld JP: 2008, Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study. Arch Virol 153:69-79.
80. Press CM, Heggebo R, Espenes A: 2004, Involvement of gut-associated lymphoid tissue of ruminants in the spread of transmissible spongiform encephalopathies. Adv Drug Deliv Rev 56:885-899.
81. Prusiner SB: 1982, Novel proteinaceous infectious particles cause scrapie. Science 216:136-144.
82. Prusiner SB: 1998, Prions. Proc Natl Acad Sci USA 95: 13363-13383.
83. Prusiner SB, Cochran SP, Alpers MP: 1985, Transmission of scrapie in hamsters. J Infect Dis 152:971-978.
84. Prusiner SB, Williams ES, Laplance JL, Sinagawa M: 2004, Scrapie, chronic wasting disease, and transmissible mink encephalopathy. In: Prion biology and disease, ed. Prusiner SB, 2nd ed., pp. 545-594. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
85. Richt JA, Hall SM: 2008, BSE case associated with prion protein gene mutation. PLoS Pathog 4:e1000156.
86. Richt JA, Kunkle RA, Alt D, et al.: 2007, Identification and characterization of two bovine spongiform encephalopathy cases diagnosed in the United States. J Vet Diagn Invest 19:142-154.
87. Robinson MM, Hadlow WJ, Huff TP, et al.: 1994, Experimental infection of mink with bovine spongiform encephalopathy. J Gen Virol 75(pt 9):2151-2155.
88. Robinson MM, Hadlow WJ, Knowles DP, et al.: 1995, Experimental infection of cattle with the agents of transmissible mink encephalopathy and scrapie. J Comp Pathol 113: 241-251.
89. Saegerman C, Speybroeck N, Roels S, et al.: 2004, Decision support tools for clinical diagnosis of disease in cows with suspected bovine spongiform encephalopathy. J Clin Microbiol 42:172-178.
90. Schneider K, Fangerau H, Michaelsen B, Raab WH: 2008, The early history of the transmissible spongiform enceph-alopathies exemplified by scrapie. Brain Res Bull 77: 343-355.
91. Scott AC, Wells GA, Stack MJ, et al.: 1990, Bovine spongiform encephalopathy: detection and quantitation of fibrils, fibril protein (PrP) and vacuolation in brain. Vet Microbiol 23:295-304.
92. Simmons MM, Harris P, Jeffrey M, et al.: 1996, BSE in Great Britain: consistency of the neurohistopathological findings in two random annual samples of clinically suspect cases. Vet Rec 138:175-177.
93. Siso S, Doherr MG, Botteron C, et al.: 2007, Neuropathologi-cal and molecular comparison between clinical and asymptomatic bovine spongiform encephalopathy cases. Acta Neuropathol 114:501-508.
94. Siso S, Gonzalez L, Jeffrey M: 2010, Neuroinvasion in prion diseases: the roles of ascending neural infection and blood dissemination. Interdiscip Perspect Infect Dis 2010:747892.
95. Smith JD, Greenlee JJ, Hamir AN, Greenlee MH: 2009, Altered electroretinogram b-wave in a Suffolk sheep experimentally infected with scrapie. Vet Rec 165:179-181.
96. Smith JD, Greenlee JJ, Hamir AN, West Greenlee MH: 2008, Retinal cell types are differentially affected in sheep with scra-pie. J Comp Pathol 138:12-22.
97. Sohn HJ, Lee YH, Green RB, et al.: 2009, Bone marrow infec-tivity in cattle exposed to the bovine spongiform encephalopa-thy agent. Vet Rec 164:272-273.
98. Spraker TR, Balachandran A, Zhuang D, O'Rourke KI: 2004, Variable patterns of distribution of PrP(CWD) in the obex and cranial lymphoid tissues of Rocky Mountain elk (Cervus elaphus nelsoni) with subclinical chronic wasting disease. Vet Rec 155:295-302.
99. Stack M, Focosi-Snyman R, Cawthraw S, et al.: 2009, Two unusual bovine spongiform encephalopathy cases detected in Great Britain. Zoonoses Public Health 56:376-383.
100. Stack MJ, Chaplin MJ, Clark J: 2002, Differentiation of prion protein glycoforms from naturally occurring sheep scrapie, sheep-passaged scrapie strains (CH1641 and SSBP1), bovine spongiform encephalopathy (BSE) cases and Romney and Cheviot breed sheep experimentally inoculated with BSE using two monoclonal antibodies. Acta Neuropathol 104: 279-286.
101. Sugiura K, Onodera T, Bradley R: 2009, Epidemiological features of the bovine spongiform encephalopathy epidemic in Japan. Rev Sci Tech 28:945-956.
102. Terry LA, Marsh S, Ryder SJ, et al.: 2003, Detection of disease-specific PrP in the distal ileum of cattle exposed orally to the agent of bovine spongiform encephalopathy. Vet Rec 152:387-392.
103. Tester S, Juillerat V, Doherr MG, et al.: 2009, Biochemical typing of pathological prion protein in aging cattle with BSE. Virol J 6:64.
104. BSE distribution in brains from BSE field cases using rapid tests. J Virol Methods 127:24-32.
105. Wells GA, Hawkins SA, Green RB, et al.: 1998, Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy (BSE): an update. Vet Rec 142: 103-106.
106. Wells GA, Hawkins SA, Green RB, et al.: 1999, Limited detection of sternal bone marrow infectivity in the clinical phase of experimental bovine spongiform encephalopathy (BSE). Vet Rec 144:292-294.
107. Wells GA, Konold T, Arnold ME, et al.: 2007, Bovine spongi-form encephalopathy: the effect of oral exposure dose on attack rate and incubation period in cattle. J Gen Virol 88:1363-1373.
108. Wells GA, Scott AC, Johnson CT, et al.: 1987, A novel progressive spongiform encephalopathy in cattle. Vet Rec 121:419-420.
109. Wells GA, Spiropoulos J, Hawkins SA, Ryder SJ: 2005, Patho-genesis of experimental bovine spongiform encephalopathy: pre-clinical infectivity in tonsil and observations on the distribution of lingual tonsil in slaughtered cattle. Vet Rec 156:401-407.
110. Wells GA, Wilesmith JW, McGill IS: 1991, Bovine spongi-form encephalopathy: a neuropathological perspective. Brain Pathol 1:69-78.
111. Wells GAH, Wielesmith JW: 2004, Bovine spongiform encephalopathy and related diseases. In: Prion biology and disease, ed. Prusiner SB, 2nd ed., pp. 595-628. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
112. Wilesmith JW, Ryan JB: 1997, Absence of BSE in the offspring of pedigree suckler cows affected by BSE in Great Britain. Vet Rec 141:250-251.
113. Wilesmith JW, Ryan JB, Atkinson MJ: 1991, Bovine spongi-form encephalopathy: epidemiological studies on the origin. Vet Rec 128:199-203.
114. Wilesmith JW, Wells GA, Cranwell MP, Ryan JB: 1988, Bovine spongiform encephalopathy: epidemiological studies. Vet Rec 123:638-644.
115. Williams ES: 2005, Chronic wasting disease. Vet Pathol 42: 530-549.
116. Williams ES, Miller MW: 2002, Chronic wasting disease in deer and elk in North America. Rev Sci Tech 21:305-316.
117. Yamakawa Y, Hagiwara K, Nohtomi K, et al.: 2003, Atypical proteinase K-resistant prion protein (PrPres) observed in an apparently healthy 23-month-old Holstein steer. Jpn J Infect Dis 56:221-222.
118. Yuan J, Xiao X, McGeehan J, et al.: 2006, Insoluble aggregates and protease-resistant conformers of prion protein in uninfected human brains. J Biol Chem 281:34848-34858.
119. Zu Rhein GM, Eckroade R, Marsh RF: 1971, Experimental transmissible mink encephalopathy (TME) in mink, monkey, and hamster. Electron microscopic studies. J Neuropathol Exp Neurol 30:124.