A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate

Journal of Chromatography A

Volumn 1218, Issue 23, 2011, Pages 3654-3659

  Li, Xiang ^a   Leong, Susanna Su Jan ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

Antimicrobial activity; Antimicrobial peptide; Human defensin 25; Refolding; Soluble aggregates

Indexed keywords

ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; DEFENSINS; REFOLDING; SOLUBLE AGGREGATES;

AGGREGATES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; PEPTIDES; POLYPEPTIDES; VIRUSES;

SIZE EXCLUSION CHROMATOGRAPHY;

BETA DEFENSIN; BETA DEFENSIN 25; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL GROWTH; BIOPROCESS; CHROMATOGRAPHY; CONTROLLED STUDY; CYTOTOXICITY TEST; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GROWTH INHIBITION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN TARGETING; PROTEIN TERTIARY STRUCTURE;

ANTI-BACTERIAL AGENTS; BETA-DEFENSINS; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; MICROBIAL SENSITIVITY TESTS; PROTEIN REFOLDING; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

FUNGI;

EID: 79955956498     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.04.017     Document Type: Article

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