Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27

Applied Microbiology and Biotechnology

Volumn 84, Issue 2, 2009, Pages 301-308

  Huang, Lei ^a   Leong, Susanna Su Jan ^a   Jiang, Rongrong ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

Antimicrobial activity; Antimicrobial peptide; Human b defensin 26; Human b defensin 27

Indexed keywords

ANTIMICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; CATIONIC ANTIMICROBIAL PEPTIDES; CATIONIC EXCHANGE; CODON OPTIMIZATION; DEFENSINS; E. COLI; EXPRESSION VECTORS; FEATURE FUSION; FUSION EXPRESSION; FUSION PROTEINS; HUMAN B-DEFENSIN-26; HUMAN B-DEFENSIN-27; N-TERMINALS; RECOMBINANT EXPRESSION; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE; SALT-SENSITIVE; STAPHYLOCOCCUS AUREUS; THIOREDOXINS;

AFFINITY CHROMATOGRAPHY; AMINES; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; PEPTIDES;

POLYPEPTIDES;

BETA DEFENSIN; BETA DEFENSIN 26; BETA DEFENSIN 27; ENTEROPEPTIDASE; THIOREDOXIN; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; BIOACTIVITY; CHROMATOGRAPHY; CLONE; GENE EXPRESSION; ION EXCHANGE; PEPTIDE; PLASMID; PROTEIN; TRANSFORMATION; YEAST;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; CODON; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; HUMAN; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE; STAPHYLOCOCCUS AUREUS;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BETA-DEFENSINS; CHROMATOGRAPHY, AFFINITY; COLONY COUNT, MICROBIAL; ENTEROPEPTIDASE; EPIDIDYMAL SECRETORY PROTEINS; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; THIOREDOXINS;

ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; SACCHAROMYCES CEREVISIAE; STAPHYLOCOCCUS AUREUS;

EID: 68749119445     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1982-z     Document Type: Article

References (27)

1. KW Bensch M Raida HJ Magert P Schulzknappe WG Forssmann 1995 Hbd-1-a novel beta-defensin from human plasma Febs Lett 368 331 335
2. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254
3. RM Epand HJ Vogel 1999 Diversity of antimicrobial peptides and their mechanisms of action Biochim Biophys Acta-Biomembr 1462 11 28
4. T Ganz 1999 Immunology-defensins and host defense Science 286 420 421
5. T Ganz ME Selsted D Szklarek SSL Harwig K Daher DF Bainton RI Lehrer 1985 Defensins-natural peptide antibiotics of human-neutrophils J Clin Invest 76 1427 1435
6. JRC Garcia A Krause S Schulz FJ Rodriguez-Jimenez E Kluver K Adermann U Forssmann A Frimpong-Boateng R Bals WG Forssmann 2001 Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity Faseb J 15 1819
7. G Hannig SC Makrides 1998 Strategies for optimizing heterologous protein expression in Escherichia coli Trends Biotech 16 54 60
8. J Harder J Bartels E Christophers JM Schroder 1997 A peptide antibiotic from human skin Nature 387 861 861
9. J Harder J Bartels E Christophers JM Schroder 2001 Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic J Biol Chem 276 5707 5713
10. L Huang CB Ching RR Jiang SSJ Leong 2008 Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression Protein Expr Purif 61 168 174
11. L Huang JF Wang ZX Zhong L Peng HQ Chen ZN Xu PL Cen 2006 Production of bioactive human beta-defensin-3 in Escherichia coli by soluble fusion expression Biotechnol Lett 28 627 632
12. HP Jia BC Schutte A Schudy R Linzmeier JM Guthmiller GK Johnson BF Tack JP Mitros A Rosenthal T Ganz PB McCray 2001 Discovery of new human beta-defensins using a genomics-based approach Gene 263 211 218
13. AS Johansson R Bolton-Grob B Mannervik 1999 Use of silent mutations in cDNA encoding human glutathione transferase M2-2 for optimized expression in Escherichia coli Protein Expr Purif 17 105 112
14. DE Jones CL Bevins 1992 Paneth cells of the human small-intestine express an antimicrobial peptide gene J Biol Chem 267 23216 23225
15. DE Jones CL Bevins 1993 Defensin-6 messenger-Rna in human paneth cells-implications for antimicrobial peptides in host defense of the human bowel Febs Lett 315 187 192
16. ER Lavallie EA Diblasio S Kovacic KL Grant PF Schendel JM McCoy 1993 A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia-coli cytoplasm Bio-Technology 11 187 193
17. L Peng ZN Xu XM Fang F Wang S Yang PL Cen 2004 Preferential codons enhancing the expression level of human beta-defensin-2 in recombinant Escherichia coli Protein Pept Lett 11 339 344
18. KL Piers MH Brown REW Hancock 1993 Recombinant-DNA procedures for producing small antimicrobial cationic peptides in bacteria Gene 134 7 13
19. FJ Rodriguez-Jimenez A Krause S Schulz WG Forssmann JR Conejo-Garcia R Schreeb D Motzkus 2003 Distribution of new human beta-defensin genes clustered on chromosome 20 in functionally different segments of epididymis Genomics 81 175 183
20. JM Schroder 1999 Epithelial antimicrobial peptides: innate local host response elements Cell Mol Life Sci 56 32 46
21. BC Schutte JP Mitros JA Bartlettt JD Walters HP Jia MJ Welsh TL Casavant PB McCray 2002 Discovery of five conserved beta-defensin gene clusters using a computational search strategy Proc Natl Acad Sci U S A 99 2129 2133
22. A Singh A Bateman QZ Zhu S Shimasaki F Esch S Solomon 1988 Structure of a novel human granulocyte peptide with anti-acth activity Biochem Biophys Res Commun 155 524 529
23. EJ Stewart F Aslund J Beckwith 1998 Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins Embo J 17 5543 5550
24. YQ Tang J Yuan G Osapay K Osapay D Tran CJ Miller AJ Ouellette ME Selsted 1999 A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated alpha-defensins Science 286 498 502
25. CG Wilde JE Griffith MN Marra JL Snable RW Scott 1989 Purification and characterization of human neutrophil peptide-4, a novel member of the defensin family J Biol Chem 264 11200 11203
26. JH Wong LX Xia TB Ng 2007 A review of defensins of diverse origins Current Protein & Peptide Science 8 446 459
27. Y Yamaguchi T Nagase R Makita S Fukuhara T Tomita T Tominaga H Kurihara Y Ouchi 2002 Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice J Immunol 169 2516 2523