High productivity refolding of an inclusion body protein using pulsed-fed size exclusion chromatography

Process Biochemistry

Volumn 45, Issue 9, 2010, Pages 1570-1576

  Chen, Yu ^a   Leong, Susanna Su Jan ^a  

^a Nanyang Technological University   (Singapore)

Author keywords

Alpha fetoprotein; Protein; Pulsed refolding; Refolding; Size exclusion chromatography

Indexed keywords

E. COLI; FETOPROTEIN; GRADIENT LENGTH; HIGH PRODUCTIVITY; INCLUSION BODIES; ION-EXCHANGE CHROMATOGRAPHY; MATRIX; PROCESS PRODUCTIVITY; PRODUCT CONCENTRATION; PROTEIN REFOLDING; PULSED INJECTION; PULSED REFOLDING; REFOLDING; SIMULTANEOUS PURIFICATION; STERIC CONSTRAINT; UREA GRADIENT;

CONCENTRATION (PROCESS); ESCHERICHIA COLI; FEEDING; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; METABOLISM; PRODUCTIVITY; PROTEINS; UREA;

SIZE EXCLUSION CHROMATOGRAPHY;

ESCHERICHIA COLI;

EID: 77955052132     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.06.010     Document Type: Article

References (25)

1. Li M., Su Z.G., Janson J.C. In vitro protein refolding by chromatographic procedures. Prot Exp Purif 2004, 33:1-10.
2. Middelberg A.P.J. Preparative protein refolding. Trends Biotechnol 2002, 20:437-443.
3. Batas D., Chaudhuri J.B. Protein refolding at high concentration using size-exclusion chromatography. Biotechnol Bioeng 1996, 50:16-23.
4. Gu Z.Y., Su Z.G., Janson J.C. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. J Chromatogr A 2001, 918:311-318.
5. Gu Z.Y., Weidenhaupt M., Ivanova N., Pavlov M., Xu B.Z., Su Z.G., et al. Chromatographic methods for the isolation of and refolding of proteins from Escherichia coli inclusion bodies. Prot Exp Purif 2002, 25:174-179.
6. Park B.J., Lee C.H., Koo Y.M. Development of novel protein refolding using simulated moving bed chromatography. Korean J Chem Eng 2005, 22:425-432.
7. Parker M.H., Birck-Wilson E., Allard G., Masiello N., Day M., Murphy K.P., et al. Purification and characterization of a recombinant version of human [alpha]-fetoprotein expressed in the milk of transgenic goats. Prot Exp Purif 2004, 38:177-183.
8. Agarwal R.K., Chan C.-C., Wiggert B., Caspi R.R. Pregnancy ameliorates induction and expression of experimental autoimmune uveitis. J Immunol 1999, 162:2648-2654.
9. Confavreux C., Hutchinson M., Hours M.M., Cortinovis-Tourniaire P., Moreau T. Rate of pregnancy-related relapse in multiple sclerosis. N Eng J Med 1998, 339:285-291.
10. Buyon J.P. The effects of pregnancy on autoimmune diseases. J Leukoc Biol 1998, 63:281-287.
11. Brenner T., Abramsky O. Immunosuppression of experimental autoimmune myasthenia-gravis by alpha-fetoprotein rich formation. Immunol Lett 1981, 3:163-167.
12. Morinaga T., Sakai M., Wegmann G., Tamaoki T. Primary structure of human α-fetoprotein and mRNA. Proc Natl Acad Sci USA 1983, 80:4604-4608.
13. Leong S.S.J., Middelberg A.P.J. A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies. Biotechnol Bioeng 2007, 97:99-117.
14. Chen Y., Leong S.S.J. Adsorptive refolding of a highly disulfide-bonded inclusion body protein using anion-exchange chromatography. J Chromatogr A 2009, 1216:4877-4886.
15. De Bernardez Clark E., Schwarz E., Rudolph R., Ronald W. Inhibition of aggregation side reactions during in vitro protein folding. Methods in enzymology, vol. 309 1999, Academic Press, pp. 217-236.
16. Leong S.S.J., Middelberg A.P.J. Dilution versus dialysis: a quantitative study of the oxidative refolding of recombinant human alpha-fetoprotein. Food Bioprod Process 2006, 84:9-17.
17. Cowley D.J., Mackin R.B. Expression, purification and characterization of recombinant human proinsulin. FEBS Lett 1997, 402:124-130.
18. Fan X.D., Xu D.S., Lu B., Xia J., Wei D.Z. Improving the refolding of NTA protein by urea gradient and arginine gradient size-exclusion chromatography. J Biochem Biophys Methods 2008, 70:1130-1138.
19. Wolynes P.G., Onuchic J.N., Thirumalai D. Navigating the folding routes. Science 1995, 267:1619-1620.
20. Dill K.A., Chan H.S. From Levinthal to pathways to funnels. Nat Struct Biol 1997, 4:10-19.
21. Chan H.S., Dill K.A. Protein folding in the landscape perspective: Chevron plots and non-Arrhenius kinetics. Prot Struct Funct Bioinform 1998, 30:2-33.
22. Dinner A.R., Sali A., Smith L.J., Dobson C.M., Karplus M. Understanding protein folding via free-energy surfaces from theory and experiment. Trends Biochem Sci 2000, 25:331-339.
23. Dobson C.M. Protein folding and misfolding. Nature 2003, 426:884-890.
24. Righetti P.G., Verzola B. Folding/unfolding/refolding of proteins: present methodologies in comparison with capillary zone electrophoresis. Electrophoresis 2001, 22:2359-2374.
25. Buswell A.M., Middelberg A.P.J. Critical analysis of lysozyme refolding kinetics. Biotechnol Prog 2002, 18:470-475.