Overpressured layer chromatography (OPLC) - A flexible tool of analysis and isolation

Natural Product Communications

Volumn 6, Issue 5, 2011, Pages 719-732

  Mincsovics, Emil ^a,b   Tyihák, Erno ^c  

^a OPLC NIT Ltd   (Hungary)

^b Institute of Entrepreneurship and Innovation   (Hungary)

^c INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Analysis; Biological detection; Infusion and transfusion OPLC; Isolation; Off line and on line processes

Indexed keywords

ANALYTIC METHOD; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; ISOLATION PROCEDURE; METHODOLOGY; ONLINE SYSTEM; OVERPRESSURED LAYER CHROMATOGRAPHY; REVIEW; SEPARATION TECHNIQUE;

EID: 79955793924     PISSN: 1934578X     EISSN: 15559475     Source Type: Journal    
DOI: 10.1177/1934578x1100600528     Document Type: Review

References (105)

1. Tyihák E, Held G. (1971) Thin-layer chromatography in pharmacognosy. In Progress in TLC and Related Techniques. Vol. II, Niederwieser A, Pataki G. (Eds), Ann Arbor Science Publishers, Ann Arbor, MI. 183-234.
2. Tyihák E, Mincsovics E, Kalász H. (1979) New planar liquid chromatographic technique: overpressured thin-layer chromatography. Journal of Chromatography, 174, 75-81.
3. Mincsovics E, Tyihák E, Kalász H. (1980) Resolution and retention behavior of some dyes in overpressured thin-layer chromatography. Journal of Chromatography, 191, 293-300.
4. Kalász H, Nagy J, Tyihák E, Mincsovics E. (1980) Circular development with overpressured thin-layer chromatography, Journal of Liquid Chromatography, 3, 845-890.
5. Tyihák E, Mincsovics E. (1988). Forced-flow planar liquid chromatographic techniques. Journal of Planar Chromatography - Modern TLC, 1, 6-19.
6. Tyihák E, Mincsovics E. (2010) Forced-flow planar liquid chromatographic techniques (after twenty-two years). Journal of Planar Chromatography - Modern TLC, 23, 382-395.
7. Mincsovics E. (2000) Efficiency characteristics of off-line infusion and conventional transfusion OPLC. In Proceedings of Planar Chromatography 2000, Nyiredy Sz, Kakuk Á. (Eds) Research Institute for Medicinal Plants, Budakalász, Hungary. 109-119.
8. Mincsovics E, Sárdi É, Velich I, Kátay Gy, Tyihák E. (2002) Micro-preparative OPLC - Rapid isolation by transfusion and infusion-transfusion processes, Journal of Planar Chromatography - Modern TLC, 15, 280-285. (Pubitemid 35153675)
9. Tyihák E, Mincsovics E, Körmendi F. (1983) Overpressured layer chromatography. I. Basic principles, instruments and developments. Hungarian Scientific Instruments, 55, 33-39.
10. Tyihák E (1987). Overpressured layer chromatography and its applicability in pharmaceutical and biomedical analysis, Journal of Pharmaceutical and Biomedical Analysis, 5, 191-203.
11. Mincsovics E, Garami M, Kecskés L, Tapa B, Végh Z, Kátay Gy, Tyihák E. (1999). Personal overpressured-layer chromatography (OPLC) basic system 50, flexible tool in analytical and semipreparative work. Journal of AOAC International, 82, 587-598. (Pubitemid 129560914)
12. Tyihák E, Mincsovics E, Székely TJ. (1989) Overpressured multi-layer chromatography. Journal of Chromatography, 471, 375-387.
13. Botz L, Nyiredy S, Sticher O. (1990) The principles of long distance OPLC, a new multi-layer development technique. Journal of Planar Chromatography - Modern TLC, 3, 352-354.
14. Botz L, Nyiredy S, Sticher O. (1991) Applicability of long distance overpressured layer chromatography. Journal of Planar Chromatography - Modern TLC, 4, 115-122.
15. Nyiredy Sz. (2001) The bridge between TLC and HPLC: overpressured layer chromatography (OPLC). Trends in Analytical Chemistry, 20, 91-101.
16. Mincsovics E, Tyihák E, Siouffi AM. (1988) Comparison of off-line and on-line overpressured layer chromatography (OPLC). Journal of Planar Chromatography - Modern TLC, 1, 141-145.
17. Oroszlán P, Verzár-Petri G, Mincsovics E, Székely T. (1987) Separation, quantitation and isolation of cannabinoids from Cannabis sativa L. by overpressured layer chromatography. Journal of Chromatography, 388, 217-224.
18. Mincsovics E, Tyihák E, Siouffi A. (l986) Characteristics of the one-dimensional on-line separation and detection in a modified CHROMPRES chamber. In Proceedings of International Symposium on TLC with Special Emphasis on Overpressured Layer Chromatography (OPLC). Tyihák E (Ed). Labor MIM, Budapest, 251-264.
19. Nyiredy Sz, Erdelmeier CAJ, Dallenbach-Toelke K, Nyiredy-Mikita K, Sticher O. (1986) Preparative on-line overpressure layer chromatography (OPLC): a new separation technique for natural products. Journal of Natural Products, 49, 885-891.
20. Mincsovics E, Tyihák E. (1988) Analytical and preparative overpressured layer chromatography (OPLC). In Recent Advances in Thin-Layer Chromatography, Dallas FAA, Read H, Ruane RJ, Wilson ID (Eds). Plenum Press, New York, London, 57-65.
21. Mincsovics E, Manach M, Kecskés L, Tapa B, Papillard D, Tyihák E. (2003) Single- and multi-channel OPLC separation on nonsegmented sorbent bed using flowing eluent wall for operating segmentation. Journal of Liquid Chromatography & Related Technologies, 26, 2593-2609.
22. Mincsovics E. (2004) Flowing eluent wall processes in OPLC: Using segmentation of non-segmented adsorbent layer for single and parallel separations. Journal of Planar Chromatography - Modern TLC, 17, 411-419.
23. Mincsovics E. (2008) Potential of the flowing eluent wall and aliquot on-line detection in the OPLC 50 and 100 Systems. Journal of Planar Chromatography - Modern TLC, 21, 97-102.
24. Mincsovics E. (2008) Forced-flow planar layer liquid chromatographic techniques for the separation and isolation of natural substances. In Thin Layer Chromatography in Phytochemistry. Waksmundzka-Hajnos M, Sherma J, Kowalska T. (Eds), CRC Press, Boca Raton, London, New York, 215-251.
25. Tyihák E, Mincsovics E, Kalász H, Nagy J. (1981) Optimization of operating parameters in overpressured thin-layer chromatography. Journal of Chromatography, 211, 45-51.
26. Nyiredy Sz, Mészáros S, Dallenbach-Toelke K, Nyiredy-Mikita K, Sticher O. (1987) The 'disturbing zone' in overpressure layer chromatography (OPLC). Journal of High Resolution Chromatography & Chromatography Communications, 10, 352-356. (Pubitemid 17620770)
27. Niederwieser A, Brenner M. (1965) Polyzonal thin-layer chromatography. Chromatographic separation of non-solid mixtures and its use in the separation of solid mixtures. I. 2-zone thin-layer chromatography. Theory and practice, Experientia, 21, 50-54.
28. Tabanca N, Demirci B, Baser KHC, Mincsovics E, Khand SI, Jacob MR, Wedge DE. (2007) Characterization of volatile constituents of Scaligeria tripartita and studies on the antifungal activity against phytopathogenic fungi. Journal of Chromatography B, 850, 221-229. (Pubitemid 46618167)
29. Nyiredy S. Fatér Z. (1994) The elimination of typical problems associated with overpressured layer chromatography (OPLC). Journal of Planar Chromatography - Modern TLC, 7, 329-333.
30. Guiochon G, Siouffi AM. (1978), Study of the performances of thin layer chromatography II. Band broadening and plate height equation. Journal of Chromatographic Science, 16, 470-481. (Pubitemid 9393526)
31. Hauck HE, Jost W. (1983) Investigations and results obtained with overpressured thin-layer chromatography. Journal of Chromatography, 262, 113-120.
32. Tyihák E, Mincsovics E, Siouffi AM. (1990) Effect of the external pressure on the efficiency of off-line and on-line OPLC. Journal of Planar Chromatography - Modern TLC, 3, 121-125.
33. Guiochon G, Siouffi AM. (1982) Study of the performances of thin-layer chromatography. Spot capacity in thin-layer chromatography. Journal of Chromatography, 245, 1-20.
34. Mincsovics E, Tyihák E. (1988) Combination of off-line and on-line operating steps in OPLC. Journal of Planar Chromatography - Modern TLC, 1, 309-312.
35. Geiss F. (1987) Fundamentals of Thin-Layer Chromatography, Planar Chromatography, Hüthig, Heidelberg.
36. Tyihák E, Petro-Turza M, Kardos K, Mincsovics E. (1992) Behavior of the dinitrophenylhydrazones of saturated aldehydes and ketones in normal and reversed phase TLC and OPLC. Journal of Planar Chromatography - Modern TLC, 5, 376-382.
37. Kaiser RE. (1987) Einführung in die HPPLC, Hüthig, Heidelberg.
38. Witkiewicz Z, Mazurek M, Bladek J. (1993) A new instrument for overpressured layer chromatography. Journal of Planar Chromatography - Modern TLC, 6, 407-412.
39. Mincsovics E, Ferenczi-Fodor K, Tyihák E. (2003) Overpressured Layer Chromatography. In Handbook of Thin-Layer Chromatography, Sherma J, Fried B. (Eds.), Marcel Dekker, New York, Third Edition, 175-205.
40. Vuorela H, Dallenbach-Toelke K, Sticher O, Hiltunen H. (1988) Separation of the main coumarins of Peucedanum palustre with various planar chromatographic methods. Journal of Planar Chromatography - Modern TLC, 1, 123-127.
41. Zogg G, Nyiredy Sz, Sticher O. (1987) Overpressured layer chromatographic (OPLC) separation of closely related furocoumarins. Journal of Liquid Chromatography, 10, 3605-3621. (Pubitemid 18071123)
42. Salles C, Jallagens J-C, Crouzet J. (1990) Chromatographic separation and partial identification of glycosidically bound volatile components of fruit. Journal of Chromatography, 522, 255-265.
43. Nagy-Turák A, Végh Z. (1994) Extraction and in situ densitometric determination of alkaloids from Catharanthus roseus by means of overpressured layer chromatography on amino-bonded silica layers. 1. Optimization and validation of the separation system. Journal of Chromatography A, 668, 501-507.
44. Erdelmeier CAJ, Erdelmeier I, Kinghorn AD, Farnsworth NR. (1986) Use of overpressure layer chromatography (OPLC) for the separation of natural products with antineoplastic activity. Journal of Natural Products, 49, 1133-137. (Pubitemid 17041093)
45. Szabady B, Hidvégi E, Nyiredy Sz. (2002) Determination of neutral cannabinoids in hemp samples by overpressured-layer chromatography, Chromatographia (Suppl.), 56, 165-168.
46. Ludwiczuk A, Nyiredy Sz, Wolski T. (2005) Separation of the ginsenosides fraction obtained from the roots of Panax quinquefolium L. cultivated in Poland. Journal of Planar Chromatography - Modern TLC, 18, 104-107. (Pubitemid 40796824)
47. Kovács Á, Simon-Sarkadi L, Mincsovics E. (1998) Stepwise gradient separation and quantification of dansylated biogenic amines in vegetables using personal OPLC instrument. Journal of Planar Chromatography - Modern TLC, 11, 43-46. (Pubitemid 28116622)
48. Simon-Sarkadi L, Kocsy G, Csomós E, Jakab T, Végh Z. (2001) OPLC investigation of the effect of cold-hardening on the level of polyamines in wheat. Journal of Planar Chromatography - Modern TLC, 14, 326-329. (Pubitemid 34000376)
49. Leskó K, Simon-Sarkadi L, Stefanovits-Bányai É, Végh Z, Galiba G. (2004) OPLC analysis of polyamines in wheat seedlings under cadmium stress. Journal of Planar Chromatography - Modern TLC, 17, 435-437. (Pubitemid 40310036)
50. Janicka M, Pietras-Ożga D. (2010) Chromatographic evaluation of the lipophilicity of N-phenyltrichloroacetamide derivatives using micellar TLC and OPLC. Journal of Planar Chromatography - Modern TLC, 23, 396-399.
51. Tyihák E, Trézl L, Szende B. (1998) Formaldehyde cycle and the phases of stress syndrome. Annals of the New York Academy of Sciences, 851, 259-270. (Pubitemid 28370388)
52. Rózyło TK, Siembida R, Németh ZsI, Albert L, Tyihák E. (2000) HPLC-OPLC-MS investigation of change of formaldehyde and its generators in human teeth of different physiological stage. Biomedical Chromatography, 14, 173-179.
53. Yang M-H, Blunden G, Tyihák E. (1998) Formaldehyde from marine algae. Biochemical Systematics and Ecology, 26, 117-123. (Pubitemid 28231027)
54. Pelander A, Ojanperä I, Vuori E. (1998) Analysis of cyanobacterial hepatotoxins by overpressured layer chromatography. Journal of Planar Chromatography - Modern TLC, 11, 365-369. (Pubitemid 28562430)
55. Kátay Gy, Mincsovics E, Szókán Gy, Tyihák E. (1997) Comparison of thin-layer chromatography and overpressured layer chromatographic techniques for the separation of ascorbigen and 1′-methylascorbigen. Journal of Chromatography A, 764, 103-109.
56. Kátay Gy, Németh Zs, Szani Sz, Köck O, Albert L, Tyihák E. (2004) Overpressured-layer chromatographic determination of ascorbigen (bound vitamin C) in Brassica vegetables. Journal of Planar Chromatography - Modern TLC, 17, 360-364.
57. Kátay Gy, Németh Zs, Kátay E, Tyihák E. (2006) Identification of 1′-methylascorbigen in broccoli. Journal of Planar Chromatography - Modern TLC, 19, 139-145.
58. Tamburini E, Bernardi T, Granini M, Vaccari G. (2006) Separation and quantitative determination of aldoses and alditols by overpressured layer chromatography (OPLC). Journal of Planar Chromatography - Modern TLC, 19, 10-14.
59. Tamburini E, Bernardi T, Bianchini E, Pedrini P. (2009) Fermentation monitoring based on HPTLC-OPLC. The effect of a complex biological matrix on quantitative performance. Journal of Planar Chromatography - Modern TLC, 22, 9-14.
60. Ferenczi-Fodor K, Mahó S, Pap-Sziklay S, Török I, Borka L. (1997) Purity testing of levonorgestrel/norgestgrel bulk drug substances by personal OPLC. Pharmeuropa, 9, 736-742.
61. Szikszay Z, Végh Z, Ferenczi-Fodor K. (1998) Quantitative purity test for phthaloyl amlodipine by personal OPLC. Journal of Planar Chromatography - Modern TLC, 11, 428-432. (Pubitemid 29070308)
62. Wiszkidenszky A, Mahó S, Végh Z, Ferenczi-Fodor K. (1998) Validated, stability-indicating semiquantitative purity test for allylestrenol drug substance and tablet by personal OPLC. Journal of Planar Chromatography - Modern TLC, 11, 463-466. (Pubitemid 29070316)
63. Bagocsi B, Fábián O, Lauko A, Mahó S, Végh Z, Ferenczi-Fodor K. (2002) Comparison of OPLC and other chromatographic methods (TLC, HPLC and GC) for in-process purity testing of Nandrolone. Journal of Planar Chromatography - Modern TLC, 15, 252-257. (Pubitemid 35153672)
64. Katona Z, Vincze L, Végh Z, Trompler A, Ferenczi-Fodor K. (2000) Cleaning validation procedure eased by using overpressured layer chromatography. Journal of Pharmaceutical and Biomedical Analysis, 22, 349-353. (Pubitemid 30109355)
65. Szücs Z, Szabady B, Szatmáry M, Cimpan G, Nyiredy Sz. (2002) High-throughput analytical strategy with combined planar and column liquid chromatography for improvement of the poppy (Papaver somniferum L.) with a high alkaloid content. Chromatographia (Suppl.), 56, 49-54.
66. Härmälä P, Botz L, Sticher O, Hiltunen R. (1990) Two-dimensional planar chromatographic separation of a complex mixture of closely related coumarins from the genus Angelica. Journal of Planar Chromatography - Modern TLC, 3, 515-520.
67. Gombosuren N, Novák Z, Kotschy A, Mincsovics E, Dibó G. (2007) A multidimensional overpressured layer chromatographic method for the characterization of tetrazine libraries. Journal of Biochemical and Biophysical Methods, 69, 239-249. (Pubitemid 46054161)
68. Pothier J, Galand N, Tivollier R, Viel C. (1993) Separation of quaternary alkaloids in plant extracts by overpressured layer chromatography. Journal of Planar Chromatography - Modern TLC, 6, 220-222.
69. Linard A, Guesnet P, Durand G. (1993) Separation of the classes of phospholipid by overpressured layer chromatography (OPLC). Journal of Planar Chromatography - Modern TLC, 6, 322-323.
70. Tyihák E, Petro-Turza M, Kardos K, Mincsovics E. (1992) Behavior of the dinitrophenylhydrazones of saturated aldehydes and ketones in normal and reversed phase TLC and OPLC. Journal of Planar Chromatography - Modern TLC, 5, 376-382.
71. Mincsovics E, Dibó G. (1999) Rapid screening of synthetic peptides by use of the personal OPLC system. Journal of Planar Chromatography - Modern TLC, 12, 150-151. (Pubitemid 29258868)
72. Tyihák E, Kátay Gy, Ostorics Zs, Mincsovics E. (1998) Analysis of amino acids by personal OPLC instrument: 1. Separation of the main protein amino acids in a double-layer system. Journal of Planar Chromatography - Modern TLC, 11, 5-11.
73. Pelander A, Ojanperä I, Sistonen J, Sunila P. (2001) Improved identification by in situ UV spectra in planar chromatography. Journal of Liquid Chromatography and Related Technologies, 10, 3605-3621.
74. Pelander A, Backström D, Ojanperä I. (2007) Qualitative screening for basic drugs in autopsy liver samples by dual-plate overpressured layer chromatography. Journal of Chromatography B, 857, 337-340. (Pubitemid 47444586)
75. Kátay Gy, Szécsi Á, Tyihák E. (2001) Separation of fumonisins by OPLC. Journal of Planar Chromatography - Modern TLC, 14, 53-56.
76. Papp E, Farkas A, Otta K-H, Mincsovics E. (2000) Validation and robustness testing of an OPLC method for the determination of aflatoxins in wheat. Planar Chromatography - Modern TLC, 13, 328-332. (Pubitemid 32220032)
77. Otta K-H, Papp E, Bagócsi B. (2000) Determination of aflatoxins in food by overpressured-layer chromatography. Journal of Chromatography A, 882, 11-16. (Pubitemid 30323692)
78. Papp E, Otta H-K, Záray Gy, Mincsovics E. (2002) Liquid chromatographic determination of aflatoxins. Microchemical Journal, 73, 39-46.
79. Móricz ÁM, Fatér Zs, Otta K-H, Tyihák E, Mincsovics E. (2007) Overpressured layer chromatographic determination of aflatoxin B1, B2, G1 and G2 in red paprika. Microchemical Journal, 85, 140-144. .
80. Szúnyog J, Mincsovics E, Hazai I, Klebovich I. (1998) A new tool in planar chromatography: Combination of OPLC and DAR for fast separation and detection of metabolites in biological samples. Journal of Planar Chromatography - Modern TLC, 11, 25-29. (Pubitemid 28116618)
81. Dalmadi-Kiss B, Mincsovics E, Balogh-Nemes K, Klebovich I. (2000) Application of OPLC-DAR and HPLC-RD techniques in metabolite research. Journal of Planar Chromatography - Modern TLC, 13, 257-260.
82. Ludányi K, Vékey K, Szúnyog J, Mincsovics E, Karancsi T, Újszászy K, Balogh-Nemes K, Klebovich I. (1999) Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism. Journal of AOAC International, 82, 231-238.
83. Klebovich I, Mincsovics E, Szúnyog J, Ludányi K, Karancsi T, Újszászy K, Dalmadi-Kiss B, Vékey K. (1998) Isolation and identification of Deramciclane metabolites by OPLC-(DAR) on-line sample collection combined with MS techniques. Journal of Planar Chromatography - Modern TLC, 11, 394-399. (Pubitemid 28562436)
84. Morovjan G, Dalmadi-Kiss B, Klebovich I, Mincsovics E. (2002) Metabolite analysis, isolation and purity assessment using various liquid chromatographic techniques combined with radioactivity detection. Journal of Chromatographic Sciences, 40, 603-608.
85. 14C-labeled glyceryl trinitrate and its metabolites by OPLC coupled with on-line or off-line radioactivity detection. Journal of Planar Chromatography - Modern TLC, 15, 404-409.
86. Betti A, Lodi G, Bighi C, Chiorboli G, Coppi S. (1994) Use of overpressured layer chromatography and coupled OPLC-GC-MS for the analysis of acetylenic thiophene derivatives in extracts of Tagetes patula. Journal of Planar Chromatography - Modern TLC, 7, 301-304.
87. Mincsovics E, Kátay Gy, Ott PG, Király-Véghely Zs, Móricz ÁM, Tyihák E. (2005) Isolation of some antimicrobial compounds of red wine by OPLC flowing eluent wall technique. Chromatographia (Suppl.), 62, 51-56.
88. Chai W, Leteux C, Lawson AM, Stoll MS. (2003) On-line overpressure thin-layer chromatographic separation and electrospray mass spectrometric detection of glycolipids. Analytical Chemistry, 75, 118-125. (Pubitemid 36083477)
89. Mincsovics E, Pápai K, Ludányi K, Dávid ÁZ, Budai M, Antal I, Klebovich I. (2008) Fully on-line hyphenation of an experimental OPLC separation unit with diode-array detection and mass-spectrometry (OPLC-DAD-MS) for analysis of xanthine compounds. Journal of Planar Chromatography - Modern TLC, 21, 361-366.
90. Mincsovics E, Garami M, Tyihák, E. (1991) Direct coupling of OPLC with HPLC: Clean-up and separation, Journal of Planar Chromatography - Modern TLC, 4, 299-303.
91. Wedge DE, Camper ND. (2000) Connections between agro-chemicals and pharmaceuticals. In Biologically Active Natural Products: Agrochemicals and Pharmaceuticals, Cutler HG, Cutler SJ (Eds), CRC Press, Boca Raton, FL, 1-15.
92. Tyihák E, Móricz MÁ, Ott PG. (2008) Biodetection and determination of biological activity of natural products. In Thin Layer Chromatography in Phytochemistry. Waksmundzka-Hajnos M, Sherma J, Kowalska T, (Eds.) CRC Press, Boca Raton, FL, 193-214.
93. Tyihák E, Ott P, Móricz MA, Kátay Gy, Király-Véghely Zs. (2004) Antibiosis, antibiotics and formaldehyde cycle : the unique importance of planar chromatographic techniques to progress in these fields. Journal of Planar Chromatography - Modern TLC, 17, 84-88.
94. Rios JJ, Recio MC, Villar A. (1988) Screening methods for natural products with antimicrobial activity: a review of the literature. Journal of Ethnopharmacology, 23, 127-149.
95. Tyihák E, Botz L, Ott PG, Nagy S, Kocsis B, Király- Véghely Zs, Mincsovics E. (2003) The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation. Chemical Analysis (Warsaw), 48, 543-553. (Pubitemid 37000242)
96. Tyihák E, Móricz MÁ, Ott GP, Kátay Gy, Király-Véghely Zs. (2005) The potential of BioArena in the study of the formaldehydome. Journal of Planar Chromatography - Modern TLC, 18, 67-72.
97. Botz L, Nagy S, Kocsis B. (2001) Detection of microbiologically active compounds. In Planar Chromatography - A Retrospective View for the Third Millennium. Nyiredy Sz. (Ed.), Springer, Budapest, Hungary. 489-516.
98. Móricz ÁM, Ott PG, Billes F, Otta K-H, Tyihák E. (2007) The influence of L-ascorbic acid on the antibacterial-toxic activity of aflatoxins on adsorbent layer. Journal of Applied Microbiology, 103, 2525-2532.
99. Janicka M. Tyihák E, Móricz ÁM, Oscik-Mendyk B. (2008) Crucial role of formaldehyde and its reaction products in the antiproliferative activity of some potential pesticides. Journal of Planar Chromatography-Modern TLC, 21, 161-166.
100. Móricz ÁM, Horváth Gy, Molnár P, Kocsis B, Böszörményi A, Lemberkovics É, Ott PG. (2010) Investigation of thyme (Thymus vulgaris L.) essential oil by use of the BioArena system. Journal of Planar Chromatography - Modern TLC, 23, 406-410.
101. Tyihák E, Trézl L, Szende B. (1998) Formaldehyde cycle and the phases of stress syndrome. Annals of New York Academy of Sciences USA, 851, 259-270. (Pubitemid 28370388)
102. Erdelmeier CA, Van Leeuwen PA, Kinghorn AD. (1988) Phorbol diester constituents of croton oil: separation by two-dimensional TLC and rapid purification utilizing reversed-phase overpressure layer chromatography. Planta Medica, 54, 71-75. (Pubitemid 18053537)
103. Snini A, Fahimi A, Mouloungui Z, Delmas M, Gaser A. (1992) Separation and preparative isolation of phenolic dialdehydes by overpressured layer chromatography. Journal of Chromatography A, 590, 369-372.
104. Erdelmeier CA, Kinghorn AD, Farnsworth NR. (1987) On-plate injection in the preparative separation of alkaloids and glycosides. Journal of Chromatography, 389, 345-349.
105. Mincsovics E, Kiss BD, Morovjan G, Klebovich I. (2001) A new tool in metabolism research-combination of OPLC on-line radioactivity detection with HPLC-RD technique. Journal of Planar Chromatography - Modern TLC, 14, 312-317. (Pubitemid 33150300)