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Volumn 49, Issue 6, 2011, Pages 1346-1352

Lipid peroxidation, oxidative stress and acetylcholinesterase in rat brain exposed to organophosphate and pyrethroid insecticides

Author keywords

Brain; Enzymes; Insecticides; Oxidative stress; Rats

Indexed keywords

ACETYLCHOLINESTERASE; CATALASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; LACTATE DEHYDROGENASE; ORGANOPHOSPHATE; PYRETHROID; SUPEROXIDE DISMUTASE; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

EID: 79955668022     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fct.2011.03.018     Document Type: Article
Times cited : (132)

References (60)
  • 2
    • 0021318441 scopus 로고
    • Catalase in vitro
    • 3rd Ed, Lippincott-Raven publishers, Philadelphia
    • Aebi H. Catalase in vitro. Methods Enzymol. 1984, 105:121-126. 3rd Ed, Lippincott-Raven publishers, Philadelphia.
    • (1984) Methods Enzymol. , vol.105 , pp. 121-126
    • Aebi, H.1
  • 3
    • 0033062327 scopus 로고    scopus 로고
    • Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers
    • Banerjee B.D., Seth V., Bhattacharya A., Pasha S.T., Chakraborty A.K. Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers. Toxicol. Lett. 1999, 107:33-47.
    • (1999) Toxicol. Lett. , vol.107 , pp. 33-47
    • Banerjee, B.D.1    Seth, V.2    Bhattacharya, A.3    Pasha, S.T.4    Chakraborty, A.K.5
  • 4
    • 0030874440 scopus 로고    scopus 로고
    • Residual toxicity of lambda-cyhalothrin on apple foliage to Amblyseius fallacis and the tarnished plant bug, Lygus lineolaris
    • Bostanian N.J., Racette G. Residual toxicity of lambda-cyhalothrin on apple foliage to Amblyseius fallacis and the tarnished plant bug, Lygus lineolaris. Phytoparasitica 1997, 25:193-198.
    • (1997) Phytoparasitica , vol.25 , pp. 193-198
    • Bostanian, N.J.1    Racette, G.2
  • 5
    • 70449225785 scopus 로고
    • Calorimetric measurement of lactate dehydrogenase activity of body fluids
    • Cabaud P.C., Wroblewski F. Calorimetric measurement of lactate dehydrogenase activity of body fluids. J. Clin. Pathol. 1958, 30:234-236.
    • (1958) J. Clin. Pathol. , vol.30 , pp. 234-236
    • Cabaud, P.C.1    Wroblewski, F.2
  • 7
    • 59149086520 scopus 로고    scopus 로고
    • Effects of subacute exposure of dichlorvos at sublethal dosages on erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats
    • Celik I., Suzek H. Effects of subacute exposure of dichlorvos at sublethal dosages on erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats. Ecotoxicol. Environ. Saf. 2009, 72:905-908.
    • (2009) Ecotoxicol. Environ. Saf. , vol.72 , pp. 905-908
    • Celik, I.1    Suzek, H.2
  • 9
    • 33947545681 scopus 로고    scopus 로고
    • Lambda-cyhalothrin-induced changes in oxidative stress biomarkers in rabbit erythrocytes and alleviation effect of some antioxidants
    • El-Demerdash F.M. Lambda-cyhalothrin-induced changes in oxidative stress biomarkers in rabbit erythrocytes and alleviation effect of some antioxidants. Toxicol. In Vitro 2007, 21:392-397.
    • (2007) Toxicol. In Vitro , vol.21 , pp. 392-397
    • El-Demerdash, F.M.1
  • 10
    • 43049136192 scopus 로고    scopus 로고
    • Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide Pest
    • Elhalwagy M.E.A., Darwish E.M., Zaher N.S. Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide Pest. Biochem. Physiol. 2008, 91:81-89.
    • (2008) Biochem. Physiol. , vol.91 , pp. 81-89
    • Elhalwagy, M.E.A.1    Darwish, E.M.2    Zaher, N.S.3
  • 13
    • 77952951846 scopus 로고    scopus 로고
    • Effects of insecticides fenitrothion, endosulfan and abamectin on antioxidant parameters of isolated rat hepatocytes
    • El-Shenawy N.S. Effects of insecticides fenitrothion, endosulfan and abamectin on antioxidant parameters of isolated rat hepatocytes. Toxicol. In Vitro 2010, 24:1148-1157.
    • (2010) Toxicol. In Vitro , vol.24 , pp. 1148-1157
    • El-Shenawy, N.S.1
  • 14
    • 0025127633 scopus 로고
    • Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal
    • Esterbauer H., Cheeseman K.H. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990, 186:407-421.
    • (1990) Methods Enzymol. , vol.186 , pp. 407-421
    • Esterbauer, H.1    Cheeseman, K.H.2
  • 15
    • 53849134103 scopus 로고    scopus 로고
    • Effects of trichlorfon and sodium dodecyl sulphate on antioxidant defense system and acetylcholinesterase of Tilapia nilotica in vitro
    • Feng T., Li Z.B., Guo X.Q., Guo J.P. Effects of trichlorfon and sodium dodecyl sulphate on antioxidant defense system and acetylcholinesterase of Tilapia nilotica in vitro. Pest. Biochem. Physiol. 2008, 92:107-113.
    • (2008) Pest. Biochem. Physiol. , vol.92 , pp. 107-113
    • Feng, T.1    Li, Z.B.2    Guo, X.Q.3    Guo, J.P.4
  • 16
    • 64749108538 scopus 로고    scopus 로고
    • Lambda-cyhalothrin-induced biochemical and histopathological changes in the liver of rats: ameliorative effect of ascorbic acid
    • Fetoui H., Garoui E.M., Zeghal E. Lambda-cyhalothrin-induced biochemical and histopathological changes in the liver of rats: ameliorative effect of ascorbic acid. Exp. Toxicol. Pathol. 2009, 61:189-196.
    • (2009) Exp. Toxicol. Pathol. , vol.61 , pp. 189-196
    • Fetoui, H.1    Garoui, E.M.2    Zeghal, E.3
  • 17
    • 26044483798 scopus 로고    scopus 로고
    • Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos induced toxicity
    • Goel A., Dani V., Dhawan D.K. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos induced toxicity. Chem. Biol. Interact. 2005, 156:131-140.
    • (2005) Chem. Biol. Interact. , vol.156 , pp. 131-140
    • Goel, A.1    Dani, V.2    Dhawan, D.K.3
  • 18
    • 0016275313 scopus 로고
    • Glutathione S-transferases. The first enzymatic step in mercapturic acid formation
    • Habig W.H., Pabst M.J., Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 1974, 249:7130-7139.
    • (1974) J. Biol. Chem. , vol.249 , pp. 7130-7139
    • Habig, W.H.1    Pabst, M.J.2    Jakoby, W.B.3
  • 19
    • 0000163281 scopus 로고    scopus 로고
    • Glutathione in Metabolism
    • Clarendon Press, Oxford, UK, B. Halliwell, J.M.C. Gutteridge (Eds.)
    • Halliwell B., Gutteridge J.M.C. Glutathione in Metabolism. In free radicals in biology and medicine 1999, 146-150. Clarendon Press, Oxford, UK. B. Halliwell, J.M.C. Gutteridge (Eds.).
    • (1999) In free radicals in biology and medicine , pp. 146-150
    • Halliwell, B.1    Gutteridge, J.M.C.2
  • 20
    • 20344402527 scopus 로고    scopus 로고
    • Neuromechanical effects of pyrethroids, allethrin, cyhalothrin and deltamethrin on the cholinergic processes in rat brain
    • Hossain M.M., Suzukia T., Sato I., Tadashi T., Suzukib K., Kobayashi H. Neuromechanical effects of pyrethroids, allethrin, cyhalothrin and deltamethrin on the cholinergic processes in rat brain. Life Sci. 2005, 77:795-807.
    • (2005) Life Sci. , vol.77 , pp. 795-807
    • Hossain, M.M.1    Suzukia, T.2    Sato, I.3    Tadashi, T.4    Suzukib, K.5    Kobayashi, H.6
  • 21
    • 0032948625 scopus 로고    scopus 로고
    • Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species
    • Kale M., Rathore N., John S., Bhatnagar D. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol. Lett. 1999, 105:197-205.
    • (1999) Toxicol. Lett. , vol.105 , pp. 197-205
    • Kale, M.1    Rathore, N.2    John, S.3    Bhatnagar, D.4
  • 23
    • 74649083584 scopus 로고    scopus 로고
    • Malathion- induced hepatotoxicity in rats: the effects of vitamins C and E
    • Kalender S., Uzun F.G., Durak D., Demir F., Kalender Y. Malathion- induced hepatotoxicity in rats: the effects of vitamins C and E. Food Chem. Toxicol. 2010, 48:633-638.
    • (2010) Food Chem. Toxicol. , vol.48 , pp. 633-638
    • Kalender, S.1    Uzun, F.G.2    Durak, D.3    Demir, F.4    Kalender, Y.5
  • 24
    • 0036668351 scopus 로고    scopus 로고
    • Protective role of melatonin and combination of vitamin C and vitamin E on lung toxicity induced by chlorpyrifos-ethyl in rats
    • Karaoz E., Gultekin F., Akdogan M., Oncu M., Gokcimen A. Protective role of melatonin and combination of vitamin C and vitamin E on lung toxicity induced by chlorpyrifos-ethyl in rats. Exp. Toxicol. Pathol. 2002, 54:97-108.
    • (2002) Exp. Toxicol. Pathol. , vol.54 , pp. 97-108
    • Karaoz, E.1    Gultekin, F.2    Akdogan, M.3    Oncu, M.4    Gokcimen, A.5
  • 25
    • 33746018710 scopus 로고    scopus 로고
    • Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide induced liver injury
    • Khan M.S. Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide induced liver injury. Cell Biol. Funct. 2006, 24:332-372.
    • (2006) Cell Biol. Funct. , vol.24 , pp. 332-372
    • Khan, M.S.1
  • 27
    • 0020490598 scopus 로고
    • Superoxide inhibits catalase
    • Kono Y., Fridovich I. Superoxide inhibits catalase. J. Biol. Chem. 1982, 257:5751-5754.
    • (1982) J. Biol. Chem. , vol.257 , pp. 5751-5754
    • Kono, Y.1    Fridovich, I.2
  • 28
    • 0026387907 scopus 로고
    • Glutathione metabolism and its role in hepatotoxicity
    • Leve De L., Kaplowitz N. Glutathione metabolism and its role in hepatotoxicity. Pharmacol. Therap. 1991, 52:287-305.
    • (1991) Pharmacol. Therap. , vol.52 , pp. 287-305
    • Leve De, L.1    Kaplowitz, N.2
  • 29
    • 61649088812 scopus 로고    scopus 로고
    • The role of antioxidants and antioxidant related enzymes in protective responses to environmentally induced oxidative stress
    • Limon-Pacheco J., Gonsebatt M.E. The role of antioxidants and antioxidant related enzymes in protective responses to environmentally induced oxidative stress. Mutat. Res. 2009, 674:137-147.
    • (2009) Mutat. Res. , vol.674 , pp. 137-147
    • Limon-Pacheco, J.1    Gonsebatt, M.E.2
  • 32
    • 58149216649 scopus 로고    scopus 로고
    • Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc
    • Mansour S.A., Mossa A.H. Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc. Pestic. Biochem. Physiol. 2009, 93:34-39.
    • (2009) Pestic. Biochem. Physiol. , vol.93 , pp. 34-39
    • Mansour, S.A.1    Mossa, A.H.2
  • 36
    • 0015523099 scopus 로고
    • The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase
    • Misra H.P., Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 1972, 247:3170-3175.
    • (1972) J. Biol. Chem. , vol.247 , pp. 3170-3175
    • Misra, H.P.1    Fridovich, I.2
  • 37
    • 0027276544 scopus 로고
    • Evidence that rat liver microsomal glutathione transferase is responsible for glutathione-dependent protection against lipid peroxidation
    • Mosialou E., Ekstrom G., Adang A.E., Morgenstern R. Evidence that rat liver microsomal glutathione transferase is responsible for glutathione-dependent protection against lipid peroxidation. Biochem. Pharmacol. 1993, 45:1645-1651.
    • (1993) Biochem. Pharmacol. , vol.45 , pp. 1645-1651
    • Mosialou, E.1    Ekstrom, G.2    Adang, A.E.3    Morgenstern, R.4
  • 38
    • 0142088723 scopus 로고    scopus 로고
    • Different effects of Type I and Type II pyrethroids on erythrocyte plasma membrane properties and enzymatic activity in rats
    • Nasuti C., Cantalamessa F., Falcioni G., Gabbianelli R. Different effects of Type I and Type II pyrethroids on erythrocyte plasma membrane properties and enzymatic activity in rats. Toxicology 2003, 191:233-244.
    • (2003) Toxicology , vol.191 , pp. 233-244
    • Nasuti, C.1    Cantalamessa, F.2    Falcioni, G.3    Gabbianelli, R.4
  • 39
    • 0028236423 scopus 로고
    • Glutathione content, glutathione transferase activity and lipid peroxidation in acrylamide-treated neuroblastoma N1E 115 cells
    • Odland L., Romert L., Clemedson C., Walum E. Glutathione content, glutathione transferase activity and lipid peroxidation in acrylamide-treated neuroblastoma N1E 115 cells. Toxicol. In Vitro 1994, 8:263-267.
    • (1994) Toxicol. In Vitro , vol.8 , pp. 263-267
    • Odland, L.1    Romert, L.2    Clemedson, C.3    Walum, E.4
  • 40
    • 10044229462 scopus 로고    scopus 로고
    • Lack of evidence for endocrine disrupting effects in rats exposed to fenitrothion in utero and from weaning to maturation
    • Okahashi N., Sano M., Migata K., Tamano S., Higuchi H., Kamita Y., Seki T. Lack of evidence for endocrine disrupting effects in rats exposed to fenitrothion in utero and from weaning to maturation. Toxicology 2005, 206:17-31.
    • (2005) Toxicology , vol.206 , pp. 17-31
    • Okahashi, N.1    Sano, M.2    Migata, K.3    Tamano, S.4    Higuchi, H.5    Kamita, Y.6    Seki, T.7
  • 42
    • 17044438082 scopus 로고    scopus 로고
    • Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite
    • Prasanthi K., Muralidhara, Rajini P.S. Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to Fenvalerate and its metabolite. Toxicol. In Vitro 2005, 19:449-456.
    • (2005) Toxicol. In Vitro , vol.19 , pp. 449-456
    • Prasanthi, K.1    Muralidhara Rajini, P.S.2
  • 44
    • 0034084828 scopus 로고    scopus 로고
    • In vitro and in vivo cholinesterase inhibition in Lacertides by phosphonate- and phosphorothioate- type organophosphates
    • Sanchez-Hernandez J.C., Walker C.H. In vitro and in vivo cholinesterase inhibition in Lacertides by phosphonate- and phosphorothioate- type organophosphates. Pest. Biochem. Physiol. 2000, 67:1-12.
    • (2000) Pest. Biochem. Physiol. , vol.67 , pp. 1-12
    • Sanchez-Hernandez, J.C.1    Walker, C.H.2
  • 45
    • 0032190519 scopus 로고    scopus 로고
    • Liver energy metabolism of Anguilla anguilla after exposure to fenitrothion
    • Sancho E., Ferrando M.D., Fernandez C., Andreu E. Liver energy metabolism of Anguilla anguilla after exposure to fenitrothion. Ecotoxicol. Environ. Saf. 1998, 41:168-175.
    • (1998) Ecotoxicol. Environ. Saf. , vol.41 , pp. 168-175
    • Sancho, E.1    Ferrando, M.D.2    Fernandez, C.3    Andreu, E.4
  • 46
    • 3042563317 scopus 로고    scopus 로고
    • Investigation of acute toxicity of fenitrothion on peppered corydoras
    • Sarikaya R., Selvi M., Erkoc F. Investigation of acute toxicity of fenitrothion on peppered corydoras. Chemosphere 2004, 56:697-700.
    • (2004) Chemosphere , vol.56 , pp. 697-700
    • Sarikaya, R.1    Selvi, M.2    Erkoc, F.3
  • 47
    • 79955660693 scopus 로고
    • SAS, Statistical Analysis System. SAS User's Guide: Statistics, version 5 Edition SAS Inst., Inc., Cary, NC, USA.
    • SAS, 1986. Statistical Analysis System. SAS User's Guide: Statistics, version 5 Edition SAS Inst., Inc., Cary, NC, USA.
    • (1986)
  • 48
    • 0035692337 scopus 로고    scopus 로고
    • Lipid peroxidation, free radical scavenging enzymes, and glutathione redox system in blood of rats exposed to propoxur
    • Seth V., Banerjee B.D., Chakravorty A.K. Lipid peroxidation, free radical scavenging enzymes, and glutathione redox system in blood of rats exposed to propoxur. Pest. Biochem. Physiol. 2001, 71:133-139.
    • (2001) Pest. Biochem. Physiol. , vol.71 , pp. 133-139
    • Seth, V.1    Banerjee, B.D.2    Chakravorty, A.K.3
  • 49
    • 0025570409 scopus 로고
    • Brain AChE activity studies in some fish species collected from a mercury contaminated estuary
    • Shaw B.P., Panigrahi A.K. Brain AChE activity studies in some fish species collected from a mercury contaminated estuary. Water Air Soil Pollut. 1990, 53:327-334.
    • (1990) Water Air Soil Pollut. , vol.53 , pp. 327-334
    • Shaw, B.P.1    Panigrahi, A.K.2
  • 50
    • 33748509695 scopus 로고    scopus 로고
    • Behavioral and neurochemical effects induced by pyrethroid-based mosquito repellent exposure in rat offsprings during prenatal and early postnatal period
    • Sinha C., Seth K., Islam F., Kumar R., Shukla C.S., Mathur N., Srivastava Agrawal K. Behavioral and neurochemical effects induced by pyrethroid-based mosquito repellent exposure in rat offsprings during prenatal and early postnatal period. Neurotoxicol. Teratol. 2006, 28:472-481.
    • (2006) Neurotoxicol. Teratol. , vol.28 , pp. 472-481
    • Sinha, C.1    Seth, K.2    Islam, F.3    Kumar, R.4    Shukla, C.S.5    Mathur, N.6    Srivastava Agrawal, K.7
  • 51
    • 0030915777 scopus 로고    scopus 로고
    • Cypermethrin toxicity: sublethal effect of enzyme activities in a fresh water fish, Cyprinus carpio var. Communis
    • Sivakumari K., Manavalaramanujam R., Ramesh M., Lakshmi R. Cypermethrin toxicity: sublethal effect of enzyme activities in a fresh water fish, Cyprinus carpio var. Communis. J. Environ. Biol. 1997, 18:121-125.
    • (1997) J. Environ. Biol. , vol.18 , pp. 121-125
    • Sivakumari, K.1    Manavalaramanujam, R.2    Ramesh, M.3    Lakshmi, R.4
  • 54
    • 0026555743 scopus 로고
    • Comparative study on the inhibition of acetylcholinesterase activity in the freshwater fish Cyprinus carpio by mercury and zinc
    • Suresh A., Sivaramakrishna B., Victoriamma P.C., Radhakrishnaiah K. Comparative study on the inhibition of acetylcholinesterase activity in the freshwater fish Cyprinus carpio by mercury and zinc. Biochem. Int. 1992, 26:367-375.
    • (1992) Biochem. Int. , vol.26 , pp. 367-375
    • Suresh, A.1    Sivaramakrishna, B.2    Victoriamma, P.C.3    Radhakrishnaiah, K.4
  • 55
    • 0036902772 scopus 로고    scopus 로고
    • In vivo and in vitro hepatotoxicity and glutathione interactions of N-methyldithiocarbamate and N,N-dimethyl dithiocarbamate in rat
    • Thompson R.W., Valentine H.L., Valentine W.M. In vivo and in vitro hepatotoxicity and glutathione interactions of N-methyldithiocarbamate and N,N-dimethyl dithiocarbamate in rat. Toxicol. Sci. 2002, 70:269-280.
    • (2002) Toxicol. Sci. , vol.70 , pp. 269-280
    • Thompson, R.W.1    Valentine, H.L.2    Valentine, W.M.3
  • 56
    • 0343494906 scopus 로고    scopus 로고
    • A comparison of the performance of solid phase extraction techniques in recovering organophosphorous and organochloride compounds from water
    • Tolosa I., Readman J.W., Mee L.D. A comparison of the performance of solid phase extraction techniques in recovering organophosphorous and organochloride compounds from water. J. Chromatogr. 1996, 725:93-106.
    • (1996) J. Chromatogr. , vol.725 , pp. 93-106
    • Tolosa, I.1    Readman, J.W.2    Mee, L.D.3
  • 57
    • 17444375702 scopus 로고    scopus 로고
    • Residue levels of malathion and its metabolites and fenitrothion in post-harvest treatment wheat during storage, milling and baking
    • Uygun U., Koksel H., Atli A. Residue levels of malathion and its metabolites and fenitrothion in post-harvest treatment wheat during storage, milling and baking. Food Chem. 2005, 92:643-647.
    • (2005) Food Chem. , vol.92 , pp. 643-647
    • Uygun, U.1    Koksel, H.2    Atli, A.3
  • 58
    • 77952876104 scopus 로고    scopus 로고
    • Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats
    • Uzun F.G., Demir F., Kalender S., Bas H., Kalender Y. Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food Chem. Toxicol. 2010, 48:1714-1720.
    • (2010) Food Chem. Toxicol. , vol.48 , pp. 1714-1720
    • Uzun, F.G.1    Demir, F.2    Kalender, S.3    Bas, H.4    Kalender, Y.5
  • 59
    • 79955656117 scopus 로고
    • World Health Organization, Cyhalothrin, Environmental Health Criteria, 99; Geneva, Switzerland.
    • World Health Organization, 1990. Cyhalothrin, Environmental Health Criteria, 99; Geneva, Switzerland.
    • (1990)
  • 60
    • 0030956622 scopus 로고    scopus 로고
    • Human mortality in organophosphate poisoning
    • Yamashita M., Tanaka J., Ando Y. Human mortality in organophosphate poisoning. Vet. Hum. Toxicol. 1997, 39:84-85.
    • (1997) Vet. Hum. Toxicol. , vol.39 , pp. 84-85
    • Yamashita, M.1    Tanaka, J.2    Ando, Y.3


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