Reversible suppression of an essential gene in adult mice using transgenic RNA interference

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 17, 2011, Pages 7113-7118

  McJunkin, Katherine ^a,b   Mazurek, Anthony ^a   Premsrirut, Prem K ^a   Zuber, Johannes ^a,d   Dow, Lukas E ^a   Simon, Janelle ^a   Stillman, Bruce ^a,b   Lowe, Scott W ^a,b,c  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^b WATSON SCHOOL OF BIOLOGICAL SCIENCES   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d RESEARCH INSTITUTE OF MOLECULAR PATHOLOGY   (Austria)

Author keywords

Conditional knockout; Gene targeting; Mouse genetics; Mouse models; RNAi transgenics

Indexed keywords

REPLICATION FACTOR A; SHORT HAIRPIN RNA; TETRACYCLINE;

ALLELE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CYCLE ARREST; CELL PROLIFERATION; CELL REGENERATION; CONTROLLED STUDY; DNA REPLICATION; DNA RESPONSIVE ELEMENT; ESSENTIAL GENE; GENE EXPRESSION; GENE REPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM; INTESTINE VILLUS ATROPHY; LETHALITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; TRANSGENIC MOUSE; TRANSGENICS; WEIGHT REDUCTION;

ALLELES; ANIMALS; CELL CYCLE; DNA REPLICATION; FEMALE; MALE; MICE; MICE, TRANSGENIC; REPLICATION PROTEIN A; RESPONSE ELEMENTS; RNA INTERFERENCE;

MAMMALIA; MUS; MUS MUSCULUS;

EID: 79955561070     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1104097108     Document Type: Article

References (34)

1. Sage J, Miller AL, Pérez-Mancera PA, Wysocki JM, Jacks T (2003) Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature 424:223-228.
2. Schmidt-Supprian M, Rajewsky K (2007) Vagaries of conditional gene targeting. Nat Immunol 8:665-668. (Pubitemid 46965339)
3. Loonstra A, et al. (2001) Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci USA 98:9209-9214. (Pubitemid 32743895)
4. Forni PE, et al. (2006) High levels of Cre expression in neuronal progenitors cause defects in brain development leading to microencephaly and hydrocephaly. J Neurosci 26:9593-9602. (Pubitemid 44396872)
5. Silver DP, Livingston DM (2001) Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol Cell 8:233-243. (Pubitemid 32772921)
6. Dickins RA, et al. (2007) Tissue-specific and reversible RNA interference in transgenic mice. Nat Genet 39:914-921. (Pubitemid 47014488)
7. Nyabi O, et al. (2009) Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells. Nucleic Acids Res 37:e55.
8. Christoph T, et al. (2008) Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice. Mol Cell Neurosci 37:579-589.
9. Seibler J, et al. (2007) Reversible gene knockdown in mice using a tight, inducible shRNA expression system. Nucleic Acids Res 35:e54.
10. Yang W, Paschen W (2008) Conditional gene silencing in mammalian cells mediated by a stress-inducible promoter. Biochem Biophys Res Commun 365:521-527. (Pubitemid 350181692)
11. Carmell MA, Zhang L, Conklin DS, Hannon GJ, Rosenquist TA (2003) Germline transmission of RNAi in mice. Nat Struct Biol 10:91-92. (Pubitemid 36177086)
12. Lu W, Yamamoto V, Ortega B, Baltimore D (2004) Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth. Cell 119:97-108. (Pubitemid 39349323)
13. Rubinson DA, et al. (2003) A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 33:401-406. (Pubitemid 36278858)
14. Seibler J, et al. (2005) Single copy shRNA configuration for ubiquitous gene knockdown in mice. Nucleic Acids Res 33:e67.
15. Dickins RA, et al. (2005) Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat Genet 37:1289-1295. (Pubitemid 41568715)
16. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ (2005) A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci USA 102:13212-13217. (Pubitemid 41325003)
17. Premsrirut PK, (2011) et al. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145:145-158.
18. Lapik YR, Fernandes CJ, Lau LF, Pestov DG (2004) Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis. Mol Cell 15:17-29. (Pubitemid 38850216)
19. Kinoshita Y, et al. (2001) Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells. J Biol Chem 276:6656-6665. (Pubitemid 37373530)
20. Zou Y, Liu Y, Wu X, Shell SM (2006) Functions of human replication protein A (RPA): from DNA replication to DNA damage and stress responses. J Cell Physiol 208: 267-273. (Pubitemid 43993416)
21. Wold MS (1997) Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Annu Rev Biochem 66:61-92.
22. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R (2006) Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 44:23-28. (Pubitemid 43222207)
23. Hochedlinger K, Yamada Y, Beard C, Jaenisch R (2005) Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121:465-477. (Pubitemid 40692305)
24. Gossen M, et al. (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268:1766-1769.
25. Agha-Mohammadi S, et al. (2004) Second-generation tetracycline- regulatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness. J Gene Med 6:817-828. (Pubitemid 40228098)
26. Fellmann C, et al. (2011) Functional identification of optimized RNAi triggers using a massively parallel Sensor assay. Mol Cell 41:733-746.
27. Choi JH, et al. (2010) Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling. Proc Natl Acad Sci USA 107:13660-13665.
28. Zou L, Elledge SJ (2003) Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 300:1542-1548. (Pubitemid 36682880)
29. Zuber J, et al. (2011) Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi. Nat Biotechnol 29:79-83.
30. Breitman ML, et al. (1987) Genetic ablation: targeted expression of a toxin gene causes microphthalmia in transgenic mice. Science 238:1563-1565. (Pubitemid 18032470)
31. Palmiter RD, et al. (1987) Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene. Cell 50:435-443.
32. Saito M, et al. (2001) Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice. Nat Biotechnol 19:746-750. (Pubitemid 32729536)
33. Huesken D, et al. (2005) Design of a genome-wide siRNA library using an artificial neural network. Nat Biotechnol 23:995-1001. (Pubitemid 41114034)
34. McCurrach ME, Lowe SW (2001) Methods for studying pro- and antiapoptotic genes in nonimmortal cells. Methods Cell Biol 66:197-227.