Methods for studying pro- and antiapoptotic genes in nonimmortal cells

Methods in Cell Biology

Volumn , Issue 66, 2001, Pages 197-227

  McCurrach, Mila E ^a   Lowe, Scott W ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ANIMAL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL SURVIVAL; CYTOLOGY; EMBRYO; FEMALE; FIBROBLAST; FLOW CYTOMETRY; GENE TRANSFER; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOUSE; PHYSIOLOGY; PREGNANCY; RETROVIRUS;

ANIMALS; APOPTOSIS; CELL SURVIVAL; CELLS, CULTURED; EMBRYO; FEMALE; FIBROBLASTS; FLOW CYTOMETRY; FLUORESCENT DYES; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; MICE; PREGNANCY; RETROVIRIDAE; TRANSFECTION;

EID: 33847374745     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)66010-2     Document Type: Article

References (53)

1. Andreadis, S. T., Brott, D., Fuller, A. O., and Palsson, B. O. (1997). Moloney murine leukemia virus-derived retroviral vectors decay intracellularly with a half-life in the range of 5.5 to 7.5 hours. J. Virol. 71, 7541-7548.
2. Barker, J. E., Wolfe, J. H., Rowe, L. B., and Birkenmeier, E. H. (1993). Advantages of gradient vs. 5-fluorouracil enrichment of stem cells for retroviral-mediated gene transfer. Exp. Hematol. 21, 47-54.
3. Bergemann, J., Kuhlcke, K., Fehse, B., Ratz, I., Ostertag, W., and Lother, H. (1995). Excision of specific DNA-sequences from integrated retroviral vectors via site-specific recombination. Nucleic Acids Res. 23, 4451-4456.
4. Brown, J. P., Wei, W., and Sedivy, J. M. (1997). Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblasts. Science 277, 831-834.
5. Burns, J. C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J. K. (1993). Vesicular stomatitis virus glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc. Natl. Acad. Sci. USA 90, 8033-8037.
6. Cepko, C. C., and Pears, W. (1997). Overview of the retrovirus transduction system. In "Current Protocols in Molecular Biology" (F. A. Ausubel, et al., ed.), pp. 9.9.1-9.9.16. John Wiley & Sons, New York.
7. Danos, O., and Mulligan, R. C. (1988). Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85, 6460-6464.
8. Dlugosz, A. A., Glick, A. B., Tennenbaum, T., Weinberg, W. C., and Yuspa, S. H. (1995). Isolation and utilization of epidermal keratinocytes for oncogene research. Methods Enzymol. 254, 3-20.
9. DuBridge, R. B., Tang, P., Hsia, H. C., Leong, P. M., Miller, J. H., and Calos, M. P. (1987). Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell. Biol. 7, 379-387.
10. Evan, G., and Littlewood, T. (1998). A matter of life and cell death. Science 281, 1317-1322.
11. Evan, G. I., Wyllie, A. H., Gilbert, C. S., Littlewood, T. D., Land, H., Brooks, M., Waters, C., Penn, L. Z., and Hancock, D. C. (1992). Induction of apoptosis in fibroblasts by c-myc protein. Cell 69, 119-128.
12. Finer, M. H., Dull, T. J., Qin, L., Farson, D., and Roberts, M. R. (1994). A high-efficiency retroviral transduction system for primary human T lymphocytes. Blood 83, 43-50.
13. Gaines, P., and Wojchowski, D. M. (1999). pIRES-CD4t, a dicistronic expression vector for MACS- or FACS-based selection of transfected cells. Biotechniques 26, 683-688.
14. Gorczyca, W., Melamed, M. R., and Darzynkiewicz, Z. (1998). "Analysis of Apoptosis by Flow Cytometry" (M. J. Jaroszeski and R. Heller, eds.), Vol. 91, Humana Press, Totowa, NJ.
15. Hahn, W. C., Counter, C. M., Lundberg, A. S., Beijersbergen, R. L., Brooks, M. W., and Weinberg, R. A. (1999). Creation of human tumour cells with defined genetic elements. Nature 400, 464-468.
16. Harvey, D. M., and Levine, A. J. (1991). p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts. Genes Dev. 5, 2375-2385.
17. Kafri, T., van Praag, H., Ouyang, L., Gage, F. H., and Verma, I. M. (1999). A packaging cell line for lentivirus vectors. J. Virol. 73, 576-584.
18. Kamijo, T., Zindy, F., Roussel, M. F., Quelle, D. E., Downing, J. R., Ashmun, R. A., Grosveld, G., and Sherr, C. J. (1997). Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF. Cell 91, 649-659.
19. Kotani, H., Newton, P. B., Zhang, S., Chiang, Y. L., Otto, E., Weaver, L., Blaese, R. M., Anderson, W. F., and McGarrity, G. J. (1994). Improved methods of retroviral vector transduction and production for gene therapy. Hum. Gene Ther. 5, 19-28.
20. Kuefer, M. U., Look, A. T., Pulford, K., Behm, F. G., Pattengale, P. K., Mason, D. Y., and Morris, S. W. (1997). Retrovirus-mediated gene transfer of NPM-ALK causes lymphoid malignancy in mice. Blood 90, 2901-2910.
21. Le Doux, J. M., Davis, H. E., Morgan, J. R., and Yarmush, M. L. (1999). Kinetics of retrovirus production and decay. Biotechnol. Bioeng. 63, 654-662.
22. Leiden, J. M. (1995). Gene therapy: Promise, pitfalls, and prognosis. N. Engl. J. Med 333, 871-873.
23. Leon, R. P., Hedlund, T., Meech, S. J., Li, S., Schaack, J., Hunger, S. P., Duke, R. C., and DeGregori, J. (1998). Adenoviral-mediated gene transfer in lymphocytes. Proc. Natl. Acad. Sci. USA 95, 13159-13164.
24. Livingstone, L. R., White, A., Sprouse, J., Livanos, E., Jacks, T., and Tlsty, T. D. (1992). Altered cell cycle arrest and gene amplification potential accompany loss of wild-type p53. Cell 70, 923-935.
25. Lowe, S. W., Jacks, T., Housman, D. E., and Ruley, H. E. (1994). Abrogation of oncogene-associated apoptosis allows transformation of p53-deficient cells. Proc. Natl. Acad. Sci. USA 91, 2026-2030.
26. Lowe, S. W., and Lin, A. W. (2000). Apoptosis in cancer. Carcinogenesis 21, 485-495.
27. Luskey, B. D., Rosenblatt, M., Zsebo, K., and Williams, D. A. (1992). Stem cell factor, interleukin-3, and interleukin-6 promote retroviral-mediated gene transfer into murine hematopoietic stem cells. Blood 80, 396-402.
28. Martin, S. J., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., van Schie, R. C., LaFace, D. M., and Green, D. R. (1995). Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med. 182, 1545-1556.
29. McCurrach, M. E., Connor, T. M., Knudson, C. M., Korsmeyer, S. J., and Lowe, S. W. (1997). Bax-deficiency promotes drug resistance and oncogenic transformation by attenuating p53-dependent apoptosis. Proc. Natl. Acad. Sci. USA 94, 2345-2349.
30. Miller, A. D. (1990). Retrovirus packaging cells. Hum. Gene Ther. 1, 5-14.
31. Morgenstern, J. P., and Land, H. (1990). Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 18, 3587-3596.
32. Moritz, T., Patel, V. P., and Williams, D. A. (1994). Bone marrow extracellular matrix molecules improve gene transfer into human hematopoietic cells via retroviral vectors. J. Clin. Invest. 93, 1451-1457.
33. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63.
34. Mulligan, R. C. (1993). The basic science of gene therapy. Science 260, 926-932.
35. Naldini, L., Blomer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., and Trono, D. (1996). In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267.
36. Pear, W. S., Miller, J. P., Xu, L., Pui, J. C., Soffer, B., Quackenbush, R. C., Pendergast, A. M., Bronson, R., Aster, J. C., Scott, M. L., and Baltimore, D. (1998). Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. Blood 92, 3780-3792.
37. Pear, W. S., Scott, M. L., and Nolan, G. P. (1996). "Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection" (P. Robbins, ed.). Humana Press, Totowa, NY.
38. Roe, T., Reynolds, T. C., Yu, G., and Brown, P. O. (1993). Integration of murine leukemia virus DNA depends on mitosis. EMBO J. 12, 2099-2108.
39. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). "Molecular Cloning: A Laboratory Manual," 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
40. Samuelson, A. V., and Lowe, S. W. (1997). Selective induction of p53 and chemosensitivity in RB-deficient cells by E1A mutants unable to bind the RB-related proteins. Proc. Natl. Acad. Sci. USA 94, 12094-12099.
41. Sanes, J. R., Rubenstein, J. L., and Nicolas, J. F. (1986). Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J. 5, 3133-3142.
42. Schmitt, C. A., McCurrach, M. E., de Stanchina, E., Wallace-Brodeur, R. R., and Lowe, S. W. (1999). INK4a/ARF mutations promote lymphomagenesis and chemoresistance by disabling p53. Genes Dev. 13, 2670-2677.
43. Serrano, M., Lin, A. W., McCurrach, M. E., Beach, D., and Lowe, S. W. (1997). Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 88, 593-602.
44. Soengas, M. S., Alarcon, R. M., Yoshida, H., Giaccia, A. J., Hakem, R., Mak, T. W., and Lowe, S. W. (1999). Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition. Science 284, 156-159.
45. Soneoka, Y., Cannon, P. M., Ramsdale, E. E., Griffiths, J. C., Romano, G., Kingsman, S. M., and Kingsman, A. J. (1995). A transient three plasmid expression system for the production of high liter retroviral vectors. Nucleic Acids Res. 23, 628-633.
46. Tadaro, G. J., and Green, H. (1963). Quantitative studies of the growth of mouse embryo cells in culture and their development into established cell lines. J. Cell Biol. 17, 299-313.
47. Tanaka, N., Ishihara, M., Kitagawa, M., Hisashi, H., Kimura, T., Matsuyama, T., Lamphier, M. S., Alzawa, S., Mak, T. W., and Taniguchi, T. (1994). Cellular commitment to oncogene-induced transformation or apoptosis is dependent on the transcription factor IRF-1. Cell 77, 829-839.
48. Verma, I. M. (1994). Gene therapy: Hopes, hypes, and hurdles. Mol. Med. 1, 2-3.
49. Weiss, R., Teich, N., Varmus, H., and Coffin, J. (1985). "RNA Tumor Viruses." Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
50. Williams, R. S. (1995). Human gene therapy: Of tortoises and hares. Nat. Med. 1, 1137-1138.
51. Woo, M., Hakem, R., Soengas, M.S., Duncan, G. S., Shahinian, A., Kagi, D., Hakem, A., McCurrach, M., Khoo, W., Kaufman, S. A., Senaldi, G., Howard, T., Lowe, S. W., and Mak, T. W. (1998). Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes. Genes Dev. 12, 806-819.
52. Yeh, W. C., Pompa, J. L., McCurrach, M. E., Shu, H. B., Elia, A. J., Shahinian, A., Ng, M., Wakeham, A., Khoo, W., Mitchell, K., El-Deiry, W. S., Lowe, S. W., Goeddel, D. V., and Mak, T. W. (1998). FADD: Essential for embryo development and signaling from some, but not all, inducers of apoptosis. Science 279, 1954-1958.
53. Zhu, H., Fearnhead, H. O., and Cohen, G. M. (1995). An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP. 1 cells. FEBS Lett. 374, 303-308.