Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 31, 2010, Pages 13660-13665

  Choi, Jun Hyuk ^a   Lindsey Boltz, Laura A ^a   Kemp, Michael ^a   Mason, Aaron C ^b   Wold, Marc S ^b   Sancar, Aziz ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

DNA damage checkpoint; Signal transduction; Topoisomerase II binding protein 1

Indexed keywords

ATR PROTEIN; CHECKPOINT KINASE 1; DNA TOPOISOMERASE (ATP HYDROLYSING); REPLICATION FACTOR A; SINGLE STRANDED DNA; TOPOISOMERASE IIBETA BINDING PROTEIN 1; UNCLASSIFIED DRUG; ATR PROTEIN, HUMAN; CARRIER PROTEIN; CELL CYCLE PROTEIN; DNA BINDING PROTEIN; NUCLEAR PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PROTEIN KINASE; PROTEIN SERINE THREONINE KINASE; SIGNAL TRANSDUCING ADAPTOR PROTEIN; TOPBP1 PROTEIN, HUMAN;

AMINO TERMINAL SEQUENCE; ARTICLE; DNA REPLICATION; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; PRIORITY JOURNAL; CHEMISTRY; CONFORMATION; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PHOSPHORYLATION; PROTEIN BINDING; SIGNAL TRANSDUCTION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; CARRIER PROTEINS; CELL CYCLE PROTEINS; DNA REPAIR ENZYMES; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; ENZYME ACTIVATION; HELA CELLS; HUMANS; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; REPLICATION PROTEIN A; SIGNAL TRANSDUCTION;

EID: 77956375008     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1007856107     Document Type: Article

References (39)

1. Abraham RT (2001) Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes Dev 15:2177-2196. (Pubitemid 32843830)
2. Sancar A, Lindsey-Boltz LA, Unsal-Kacmaz K, Linn S (2004) Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Annu Rev Biochem 73:39-85. (Pubitemid 39050363)
3. Cimprich KA, Cortez D (2008) ATR: an essential regulator of genome integrity. Nat Rev Mol Cell Biol 9:616-627.
4. Zou L, Elledge SJ (2003) Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 300:1542-1548. (Pubitemid 36682880)
5. Wold MS (1997) Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Annu Rev Biochem 66:61-92.
6. Kim SM, Kumagai A, Lee J, Dunphy WG (2005) Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR but not the stable DNA-binding or coiled-coil domains of ATRIP. J Biol Chem 280:38355-38364. (Pubitemid 43853732)
7. Ball HL, Myers JS, Cortez D (2005) ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Mol Biol Cell 16:2372-2381.
8. Ball HL, et al. (2007) Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol Cell Biol 27:3367-3377.
9. Delacroix S, Wagner JM, Kobayashi M, Yamamoto K, Karnitz LM (2007) The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1. Genes Dev 21:1472-1477. (Pubitemid 46955719)
10. Lee J, Kumagai A, DunphyWG(2007) The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR. J Biol Chem 282:28036-28044.
11. Yan S,MichaelWM(2009) TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks. J Cell Biol 184:793-804.
12. Kumagai A, Lee J, Yoo HY, Dunphy WG (2006) TopBP1 activates the ATR-ATRIP complex. Cell 124:943-955.
13. Hashimoto Y, Tsujimura T, Sugino A, Takisawa H (2006) The phosphorylated C-terminal domain of XenopusCut5 directly mediates ATR-dependent activation of Chk1. Genes Cells 11:993-1007. (Pubitemid 44256382)
14. Xu X, et al. (2008) The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Mol Cell Biol 28:7345-7353.
15. Kim HS, Brill SJ (2001) Rfc4 interacts with Rpa1 and is required for both DNA replication and DNA damage checkpoints in Saccharomyces cerevisiae. Mol Cell Biol 21:3725-3737. (Pubitemid 32466986)
16. Majka J, Binz SK,Wold MS, Burgers PM (2006) Replication protein A directs loading of the DNA damage checkpoint clamp to 5′-DNA junctions. J Biol Chem 281:27855-27861. (Pubitemid 44414497)
17. Majka J, Niedziela-Majka A, Burgers PM (2006) The checkpoint clamp activates Mec1 kinase during initiation of the DNA damage checkpoint. Mol Cell 24:891-901. (Pubitemid 44960097)
18. Yan S, Lindsay HD, Michael WM (2006) Direct requirement for Xmus101 in ATR-mediated phosphorylation of Claspin bound Chk1 during checkpoint signaling. J Cell Biol 173:181-186.
19. MacDougall CA, Byun TS, Van C, Yee MC, Cimprich KA (2007) The structural determinants of checkpoint activation. Genes Dev 21:898-903.
20. Clarke CA, Clarke PR (2005) DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system. Biochem J 388:705-712.
21. Yoshioka K, Yoshioka Y, Hsieh P (2006) ATR kinase activation mediated by MutSalpha and MutLalpha in response to cytotoxic O6-methylguanine adducts. Mol Cell 22:501-510.
22. Shiotani B, Zou L (2009) Single-stranded DNA orchestrates an ATM-to-ATR switch at DNA breaks. Mol Cell 33:547-558.
23. Choi JH, Lindsey-Boltz LA, Sancar A (2007) Reconstitution of a human ATR-mediated checkpoint response to damaged DNA. Proc Natl Acad Sci USA 104:13301-13306.
24. Lindsey-Boltz LA, Sercin O, Choi JH, Sancar A (2009) Reconstitution of human claspin-mediated phosphorylation of Chk1 by the ATR (ataxia telangiectasia-mutated and rad3-related) checkpoint kinase. J Biol Chem 284:33107-33114.
25. Navadgi-Patil VM, Burgers PM (2008) Yeast DNA replication protein Dpb11 activates the Mec1/ATR checkpoint kinase. J Biol Chem 283:35853-35859.
26. Richard DJ, et al. (2008) Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature 453:677-681.
27. Keshav KF, Chen C, Dutta A (1995) Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex. Mol Cell Biol 15:3119-3128.
28. Haring SJ, Mason AC, Binz SK, Wold MS (2008) Cellular functions of human RPA1 Multiple roles of domains in replication, repair, and checkpoints. J Biol Chem 283:19095-19111.
29. Haring SJ, Humphreys TD, Wold MS (2010) A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression. Nucleic Acids Res 38:846-858.
30. Mason AC, et al. (2009) An alternative form of replication protein A prevents viral replication in vitro. J Biol Chem 284:5324-5331.
31. Kemp MG, et al. (2010) An alternative form of replication protein A expressed in normal human tissues supports DNA repair. J Biol Chem 285:4788-4797.
32. Choi JH, Lindsey-Boltz LA, Sancar A (2009) Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Nucleic Acids Res 37:1501-1509.
33. Garcia V, Furuya K, Carr AM (2005) Identification and functional analysis of TopBP1 and its homologs. DNA Repair 4:1227-1239. (Pubitemid 41540442)
34. Mordes DA, Glick GG, Zhao R, Cortez D (2008) TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev 22:1478-1489. (Pubitemid 351793082)
35. Mordes DA, Nam EA, Cortez D (2008) Dpb11 activates the Mec1-Ddc2 complex. Proc Natl Acad Sci USA 105:18730-18734.
36. Lee JH, Paull TT (2005) ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex. Science 308:551-554. (Pubitemid 40570585)
37. You Z, Bailis JM, Johnson SA, Dilworth SM, Hunter T (2007) Rapid activation of ATM on DNA flanking double-strand breaks. Nat Cell Biol 9:1311-1318. (Pubitemid 350042363)
38. Soutoglou E, Misteli T (2008) Activation of the cellular DNA damage response in the absence of DNA lesions. Science 320:1507-1510. (Pubitemid 351929432)
39. Choi JH, Sancar A, Lindsey-Boltz LA (2009) The human ATR-mediated DNA damage checkpoint in a reconstituted system. Methods 48:3-7.