Measuring enzyme activity in single cells

Trends in Biotechnology

Volumn 29, Issue 5, 2011, Pages 222-230

  Kovarik, Michelle L ^a   Allbritton, Nancy L ^a,b  

^a University of North Carolina at Chapel Hill   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ASSAYS; GLYCOMICS; MULTIPLE CELLULAR PROCESS; PRODUCT FORMATION; PROTEOMICS; SEPARATION-BASED ASSAYS; SINGLE CELLS;

ENZYMES; MOLECULAR BIOLOGY;

ENZYME ACTIVITY;

GLYCOSIDASE; GLYCOSYLASE; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG;

CAPILLARY ELECTROPHORESIS; CELL ASSAY; CELL HETEROGENEITY; ENZYME ACTIVITY; ENZYME ANALYSIS; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; LASER INDUCED FLUORESCENCE; MEASUREMENT; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; REVIEW; SCANNING ELECTROCHEMICAL MICROSCOPY;

CELLS; CYTOLOGICAL TECHNIQUES; ELECTROPHORESIS, CAPILLARY; ENZYMES; FLOW CYTOMETRY; MICROSCOPY;

EID: 79954682695     PISSN: 01677799     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tibtech.2011.01.003     Document Type: Review

References (80)

1. Altschuler S.J., Wu L.F. Cellular heterogeneity: do differences make a difference?. Cell 2010, 141:559-563.
2. Spiller D.G., et al. Measurement of single-cell dynamics. Nature 2010, 465:736-745.
3. Tawfik D.S. Messy biology and the origins of evolutionary innovations. Nat. Chem. Biol. 2010, 6:692-696.
4. Zernicka-Goetz M., et al. Making a firm decision: multifaceted regulation of cell fate in the early mouse embryo. Nat. Rev. Genet. 2009, 10:467-477.
5. Prussin C., et al. TH2 heterogeneity: does function follow form?. J. Allergy Clin. Immunol. 2010, 126:1094-1098.
6. Lopez-Vergès S., et al. CD57 defines a functionally distinct population of mature NK cells in the human CH56dimCD16+ NK-cell subset. Blood 2010, 116:3865-3874.
7. Cohen A.A., et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 2008, 322:1511-1516.
8. Gascoigne K.E., Taylor S.S. Cancer cells display profound intra- and interline variation in following prolonged exposure to antimitotic drugs. Cancer Cell 2008, 14:111-122.
9. Arriaga E.A. Determining biological noise via single cell analysis. Anal. Bioanal. Chem. 2009, 393:73-80.
10. Elowitz M.B., et al. Stochastic gene expression in a single cell. Science 2002, 297:1183-1186.
11. Taniguchi Y., et al. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 2010, 329.
12. Newman J.R.S., et al. Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise. Nature 2006, 441:840-846.
13. Losick R., Desplan C. Stochasticity and cell fate. Science 2008, 320:65-68.
14. Raser J.M., O'Shea E.K. Control of stochasticity in eukaryotic gene expression. Science 2004, 304:1181-1814.
15. Ozbudak E.M., et al. Regulation of noise in the expression of a single gene. Nat. Genet. 2002, 31:69-73.
16. Krutzik P.O., et al. High-content single-cell drug screening with phosphospecific flow cytometry. Nat. Chem. Biol. 2008, 4:132-142.
17. Sun X., Jin W. Catalysis-electrochemical determination of zeptomole enzyme and its application for single-cell analysis. Anal. Chem. 2003, 75:6050-6055.
18. Yashphe J., Halvorson H.O. β-D-galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis. Science 1976, 191:1283-1284.
19. Invitrogen The Molecular Probes Handbook: a Guide to Fluorescent Probes and Labeling Technologies 2010, Invitrogen. 11th edn.
20. Ni Q., et al. Analyzing protein kinase dynamics in living cells with FRET reporters. Methods 2006, 40:279-286.
21. Kiyokawa E., et al. Fluorescence (Förster) resonance energy transfer imaging of oncogene activity in living cells. Cancer Sci. 2006, 97:8-15.
22. Frommer W.B., et al. Genetically encoded biosensors based on engineered fluorescent proteins. Chem. Soc. Rev. 2009, 28:2833-2841.
23. Ouyang M., et al. Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer. Cancer Res. 2010, 70:2204-2212.
24. Sharma V., et al. Peptide-based fluorescent sensors of protein kinase activity: design and applications. Biochim. Biophys. Acta 2008, 1784:94-99.
25. Kasili P.M., et al. Optical sensor for the detection of caspase-9 activity in a single cell. J. Am. Chem. Soc. 2004, 126:2799-2806.
26. Jiang T., et al. Tumor imaging by means of proteolytic activation of cell-penetrating peptides. Proc. Natl. Acad. Sci. U.S.A. 2004, 101:17867-17872.
27. Hobson J.P., et al. Imaging specific cell-surface proteolytic activity in single living cells. Nat. Methods 2006, 3:259-261.
28. Werner J.M., Steinfelder H.J. A microscopic technique to study kinetics and concentration-response of drug-induced caspase-3 activation on a single cell level. J. Pharmacol. Toxicol. 2008, 57:131-137.
29. Wudl L., Paigen K. Enzyme measurements on single cells. Science 1974, 184:992-994.
30. He M., et al. Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. Anal. Chem. 2005, 77:1539-1544.
31. Baret J.-C., et al. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 2009, 9:1850-1858.
32. Madou M. Fundamentals of Microfabrication 2002, Vol. 1. CRC Press. 2nd edn.
33. El-Ali J., et al. Cells on chips. Nature 2006, 442:403-411.
34. Di Carlo D., et al. Single-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arrays. Anal. Chem. 2006, 78:4925-4930.
35. Sasuga Y., et al. Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells. Anal. Chem. 2008, 80:9141-9149.
36. Zhang X., et al. Quantitative determination of enzyme activity in single cells by scanning microelectrode coupled with a nitrocellulose film-covered microreactor by means of a scanning electrochemical microscope. Anal. Chem. 2007, 79:1256-1261.
37. Gao N., et al. Scanning electrochemical microscopy coupled with intracellular standard addition method for quantification of enzyme activity in single intact cells. Analyst 2007, 132:1139-1146.
38. Campbell R.E. Fluorescent-protein-based biosensors: modulation of energy transfer as a design principle. Anal. Chem. 2009, 81:5972-5979.
39. Honda A., et al. Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:2437-2442.
40. Green H.M., Alberola-Ila J. Development of ERK activity sensor, an in vitro, FRET-based sensor of extracellular regulated kinase activity. BMC Chem. Biol. 2005, 5:1-8.
41. O'Connor J.-E., et al. The relevance of flow cytometry for biochemical analysis. IUBMB Life 2001, 51:231-239.
42. Shapiro H.M. Practical flow cytometry 2003, Wiley-Liss. 4th edn.
43. Dolbeare F. Dynamic assay of enzyme activities in single cells by flow cytometry. J. Histochem. Cytochem. 1979, 27:1644-1646.
44. Watson J.V. Enzyme kinetic studies in cell populations using fluorogenic substrates and flow cytometric techniques. Cytometry 1980, 1:143-151.
45. Severin E., Seidler E. Flow cytometric assay of cytochemically demonstrated NAD(P)H oxidoreductase (diaphorase) activities. J. Histochem. Cytochem. 1998, 46:761-765.
46. Telford W.G., et al. Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate. Cytometry 1999, 37:314-319.
47. Berger J., et al. Glucocerebrosidase deficiency dramatically impairs human bone marrow haematopoiesis in an in vitro model of Gaucher disease. Br. J. Haematol. 2010, 150:93-101.
48. Gao N., et al. High-throughput single-cell analysis for enzyme activity without cytolysis. Anal. Chem. 2006, 78:3213-3220.
49. Haddad P.R., Robards K. Inorganic species. Chromatography: Fundamentals and Applications of Chromatography and Related Differential Migration methods 2004, 1156. Elsevier. 6th edn. E. Heftmann (Ed.).
50. Perfetto S.P., et al. Seventeen-colour flow cytometry: unravelling the immune system. Nat. Rev. Immunol. 2004, 4:648-655.
51. Xue Q., Yeung E.S. Variability in intracellular lactate dehydrogenase isoenzymes in single human erythrocytes. Anal. Chem. 1994, 66:1175-1178.
52. Sun X., et al. Measurement of alkaline phosphatase isoenzymes in individual mouse bone marrow fibroblast cells based on capillary electrophoresis with on-capillary enzyme-catalyzed reaction and electrochemical detection. Electrophoresis 2004, 25:1860-1866.
53. Brown R.B., Audet J. Current techniques for single-cell lysis. J. R. Soc. Interface 2008, 5:S131-S138.
54. Luzzi V., et al. Localized sampling of cytoplasm for Xenopus oocytes for capillary electrophoresis. Anal. Chem. 1997, 69:4761-4767.
55. Shoemaker G.K., et al. Multiple sampling single-cell enzyme assays using CE-laser-induced fluorescence to monitor reaction progress. Anal. Chem. 2005, 77:3132-3137.
56. Meredith G.D., et al. Measurement of kinase activation in single mammalian cells. Nat. Biotechol. 2000, 18:309-312.
57. Jiang D., et al. Single-cell analysis of phosphoinositide 3-kinase (PI3K) and phosphatase and tensin homolog (PTEN) activation. Faraday Discuss. 2011, 149:187-200.
58. Krylov S.N., et al. Correlating cell cycle with metabolism in single cells: combination of image and metabolic cytometry. Cytometry 1999, 37:14-20.
59. Le X.C., et al. Single cell studies of enzymatic hydrolysis of a tetramethylrhodamine labeled triglucoside in yeast. Glycobiology 1999, 9:219-225.
60. Nelson A.R., et al. Myristoyl-based transport of peptides into living cells. Biochemistry 2007, 46:14771-14781.
61. Zarrine-Afsar A., Krylov S.N. Use of capillary electrophoresis and endogenous fluorescent substrate to monitor intracellular activation of protein kinase A. Anal. Chem. 2003, 75:3720-3724.
62. Marc P.J., et al. Coaxial flow system for chemical cytometry. Anal. Chem. 2007, 79:9054-9059.
63. Jiang D., et al. Microelectrophoresis platform for fast serial analysis of single cells. Electrophoresis 2010, 31:2558-2565.
64. Sims C.E., Allbritton N.L. Analysis of single mammalian cells on-chip. Lab Chip 2007, 7:423-440.
65. Zare R.N., Kim S. Microfluidic platforms for single-cell analysis. Annu. Rev. Biomed. Eng. 2010, 12:187-201.
66. Chang A., et al. BRENDA, AMENDA, and FRENDA the enzyme information system: new content and tools in 2009. Nucleic Acid Res. 2009, 37:D588-D592.
67. Arkhipov S.N., et al. Chemical cytometry for monitoring metabolism of a Ras-mimicking substrate in single cells. Cytom. Part A 2005, 63A:41-47.
68. Mann M., Jensen O.N. Proteomic analysis of post-translational modifications. Nat. Biotechnol. 2003, 21:255-261.
69. Wang D., Bodovitz S. Single cell analysis: the new frontier in '-omics'. Trends Biotechnol. 2010, 28:281-290.
70. Essentials of Glycobiology 1999, Laboratory Press, Cold Spring Harbor. A. Varki (Ed.).
71. Zheng X.T., et al. Optical detection of single cell lactate release for cancer metabolic analysis. Anal. Chem. 2010, 82:5082-5087.
72. Agnes R.S., et al. Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity. J. Am. Chem. Soc. 2010, 132:6075-6080.
73. Rehm M., et al. Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. J. Biol. Chem. 2002, 277:24506-24514.
74. Whitmore C.D., et al. Metabolic cytometry. Glycosphingolipid metabolism in single cells. Anal. Chem. 2007, 79:5139-5142.
75. Wang Q., et al. Multicolor monitoring of dysregulated protein kinases in chronic myelogenous leukemia. ACS Chem. Biol. 2010, 5:887-895.
76. Mizutani T., et al. A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. Clin. Cancer Res. 2010, 16:3964-3975.
77. Oh-hora O. Calcium signaling in the development and function of T-lineage cells. Immunol. Rev. 2009, 231:210-224.
78. Ross P.E., et al. Dynamics of ATP-induced calcium signaling in single mouse thymocytes. J. Cell Biol. 1997, 138:987-998.
79. Colman-Lerner A., et al. Regulated cell-to-cell variation in a cell-fate decision system. Nature 2005, 437:699-706.
80. Brown R.B., et al. Single amino acid resolution of proteolytic fragments generated in individual cells. Cytom. Part A 2010, 77A:347-355.