Single cell studies of enzymatic hydrolysis of a tetramethylrhodamine labeled triglucoside in yeast

Glycobiology

Volumn 9, Issue 3, 1999, Pages 219-225

  Le, X Chris ^a   Tan, Woei ^a   Scaman, Christine H ^a,b   Szpacenko, Adam ^a   Arriaga, Edgar ^a   Zhang, Yanni ^a   Dovichi, Norman J ^a   Hindsgaul, Ole ^a   Palcic, Monica M ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Capillary electrophoresis; In vivo hydrolysis; Laser induced fluorescence; Spheroplast single cell analysis; glucosidase

Indexed keywords

ALPHA GLUCOSIDASE; CASTANOSPERMINE; ENZYME INHIBITOR; GLUCOSIDE; RHODAMINE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE; HYDROLYSIS; LASER MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SPHEROPLAST; YEAST CELL;

EID: 0033014543     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.3.219     Document Type: Article

References (29)

1. Almers, W. and Neher, E. (1985) The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading. FEBS Lett., 192, 13-18.
2. Chiu, D.T., Lillard, S.J., Scheller, R.H., Zare, R.N., Rodriguez-Cruz, S.E., Williams, E.R., Orwar, O., Sandberg, M. and Lundqvist, J.A. (1998) Probing single secretory vesicles with capillary electrophoresis. Science, 279, 1190-1193.
3. Edstrom, J.-E. (1953) Nucleotide analysis on the cyto-scale. Nature, 172, 809.
4. Ewing, A.G., Strein, T.G. and Lau, Y.Y. (1992) Analytical chemistry in microenvironments: single nerve cells. Acc. Chem. Res., 25, 440-447.
5. Hoffstetter-Kuhn, S., Paulus, A., Gassmann, E. and Widmer, H.M. (1991) Influence of borate complexation on the electrophoretic behaviour of carbohydrates in capillary electrophoresis. Anal. Chem., 63, 1541-1547.
6. Honda, S., Iwase, S., Makino, A. and Fujiwara, S. (1989) Simultaneous determination of reducing monosaccharides by capillary zone electrophoresis as the borate complexes of N-2-pyridylglycamines. Anal. Biochem., 176, 72-77.
7. Jain, V.K. and Magrath, I.T. (1991) A chemiluminescent assay for quantitation of β-galactosidase in the femtogram range: application to quantitation of β-galactosidase in lacZ-transfected cells. Anal. Biochem., 199, 119-124.
8. Kennedy, R.T., Oates, M.D., Cooper, B.R., Nickerson, B. and Jorgenson, J.W. (1989) Microcolumn separations and the analysis of single cells. Science, 246, 57-63.
9. Le, X.C., Scaman, C., Zhang, Y., Zhang, J., Dovichi, N.J., Hindsgaul, O. and Palcic, M.M. (1995) Analysis by capillary electrophoresis - laser induced fluorescence detection of oligosaccharides produced from enzyme reactions. J. Chromatogr. A, 716, 215-220.
10. Le, X.C., Zhang, Y., Dovichi, N.J., Compston, C.A., Palcic, M.M., Beever, R.J and Hindsgaul, O. (1997) Study of the enzymatic transformation of fluorescently labeled oligosaccharides in human epidermoid cells using capillary electrophoresis with laser induced fluorescence detection. J. Chromatogr. A, 781, 515-522.
11. Lee, K.B., Desai, U.R., Palcic, M.M., Hindsgaul, O. and Linhardt, R.J. (1992) An electrophoresis-based assay for glycosyltransferase activity. Anal. Biochem., 205, 108-114.
12. Lillard, S.J. and Yeung, E.S. (1997) Capillary electrophoresis for the analysis of single cells: Laser-induced fluorescence detection. in Landers, J.P. (ed.), Handbook of Capillary Electrophoresis, 2nd ed. CRC Press, Boca Raton, FL, pp. 523-544.
13. Liu, J., Shirota, O., Wiesler, D. and Novotny, M. (1991) Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis. Proc. Natl. Acad. Sci. USA, 88, 2302-2306.
14. Liu, J., Shirota, O. and Novotny, M. (1992) Sensitive, laser-assisted determination of complex oligosaccharide mixtures separated by capillary gel electrophoresis at high resolution. Anal. Chem., 64, 973-975.
15. Luyten, G.P.M., Hoogeveen, A.T. and Galjaard, H. (1985) A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells. J. Histochem. Cytochem., 33, 965-968.
16. Neher, E. and Sakmann, B. (1992) The patch clamp technique. Sci. Am., 226, 44-51.
17. Neverova, I., Scaman, C.H., Srivastava, O.P., Szweda, R., Vijay, I.K. and Palcic, M.M. (1994) A spectrophotometric assay for glucosidase I. Anal. Biochem., 222, 190-195.
18. Novotny, M. and Sudor, J. (1993) High-performance capillary electrophoresis of glycoconjugates. Electrophoresis, 14, 373-389.
19. Rosenzweig, Z. and Yeung, E.S. (1994) Laser-based particle-counting microimmunoassay for the analysis of single human erythrocytes. Anal. Chem., 66, 1771-1776.
20. Rotman, B. (1961) Measurement of activity of single molecules of β-D-galactosidase. Proc. Natl. Acad. Sci. USA, 47, 1981-1991.
21. 3 for use in the assay of α-glucosidase I activity. Carbohydr. Res., 296, 203-213.
22. Stefansson, M. and Novotny, M. (1994) Separation of complex oligosaccharide mixtures by capillary electrophoresis in the open-tubular format. Anal. Chem., 66, 1134-1140.
23. Steinkamp, J.A. (1984) Flow cytometry. Rev. Sci. Instrum., 55, 1375-1400.
24. Swanek, F.D., Ferris, S.S. and Ewing, A.G. (1997) Capillary electrophoresis for the analysis of single cells: electrochemical, mass spectrometric, and radiochemical detection. In Landers, J.P. (ed.), Handbook of Capillary Electrophoresis, 2nd ed. CRC Press, Boca Raton, FL, pp. 495-521.
25. Xue, Q. and Yeung, E.S. (1994) Variability of intracellular lactate dehydrogenase isoenzymes in single human erythrocytes. Anal. Chem., 66, 1175-1178.
26. Yashphe, J. and Halvorson, H.O. (1976) β-D-Galactosidase activity in single yeast cells during cell cycle of Saccharomyces lactis. Science, 191, 1283-1284.
27. Yeung, E.S. (1994) Chemical analysis of single human erythrocytes. Acc. Chem. Res., 27, 409-414.
28. Zhang, Y., Le, X.C., Dovichi, N.J., Compston, C.A., Palcic, M.M., Diedrich, P. and Hindsgaul, O. (1995) Monitory biosynthetic transformations of N-acetyllactosamine using fluorescently labeled oligosaccharides and capillary electrophoretic separation. Anal. Biochem., 227, 368-376.
29. Zhao, J.Y., Dovichi, N.J., Hindsgaul, O., Gosselin, S. and Palcic, M.M. (1994) Detection of 100 molecules of product formed in a fucosyltransferase reaction. Glycobiology, 4, 239-242.