Inflammatory markers in childhood asthma

Clinical Chemistry and Laboratory Medicine

Volumn 49, Issue 4, 2011, Pages 587-599

  Dodig, Slavica ^a   Richter, Darko ^b   Zrinski Topić, Renata ^a  

^a SREBRNJAK CHILDREN S HOSPITAL   (Croatia)

^b KBC Zagreb   (Croatia)

Author keywords

airway inflammation; asthma; child; exhaled breath; exhaled breath condensate

Indexed keywords

BIOLOGICAL MARKER; C REACTIVE PROTEIN; EOSINOPHIL CATIONIC PROTEIN; IMMUNOGLOBULIN E; NITRIC OXIDE;

AIRWAY; ALLERGY; APOPTOSIS; ASTHMA; BLOOD ANALYSIS; BREATHING; BRONCHUS BIOPSY; CELL ACTIVATION; CELL MIGRATION; DIAGNOSTIC TEST; EOSINOPHIL; EXHALATION; FOLLOW UP; HUMAN; INFLAMMATION; INFLAMMATORY CELL; LUNG LAVAGE; OXIDATIVE STRESS; PH MEASUREMENT; PRIORITY JOURNAL; PROGNOSIS; REVIEW; SPUTUM ANALYSIS; URINALYSIS;

ASTHMA; BIOLOGICAL MARKERS; CHILD; HUMANS; INFLAMMATION; RESPIRATORY SYSTEM; SIGNAL TRANSDUCTION;

EID: 79954544507     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2011.094     Document Type: Review

References (140)

1. National Asthma Education and Prevention Program. Guidelines for the diagnosis and management of asthma. Bethesda, MD: National Heart, Lung, and Blood Institute, National Institutes of Health; 1997. Available from: http://www.nhlbi.nih.gov.
2. Global Initiative for Asthma. Global Strategy for Asthma Man-agement and Prevention. U.S. Department of Health and Human Services Public Health Service, National Institutes of Health, National Heart, Lung, and Blood Institute, NIH Publication No. 02-3659, February 2002. Available from: http://www.nhlbi.nih. gov.
3. Global Initiative for Asthma. Global Strategy for Asthma Man-agement and prevention. Updated 2009. Available from: www. ginasthma.com.
4. van Wonderen KE, van der Mark LB, Mohrs J, Bindels PJ, van Aalderen WM, Ter Riet G Different definitions in childhood asthma: how dependable is the dependent variable? Eur Respir J 2010;36:48-56
5. Global Initiative for Asthma. Global Strategy for the Diagnosis and Management of Asthma in Children 5 years and younger. Medical Communication Resources Inc., 2010. Available from: www.ginasthma.com.
6. Brand PL, Baraldi E, Bisgaard H, Boner AL, Castro-Rodriguez JA, Custovic A, et al. Definition, assessment and treatment of wheezing disorders in preschool children: an evidence-based approach. Eur Respir J 2008;32:1096-110.
7. Bacharier LB, Boner A, Carlsen K-H, Eigenmann PA, Frischer T, Gotz M, et al. (The European Pediatric Asthma Group). Diagnosis and treatment of asthma in childhood: a PRACTALL consensus report. Allergy 2008;63:5-34. (Pubitemid 350198248)
8. Martinez FD, Wright AL, Taussig LM, Holberg CJ, Halonen M, Morgan WJ. Asthma and wheezing in the first six years of life. The Group Health Medical Associates. N Engl J Med 1995;332:133-8.
9. Illi S, von ME, Lau S, Niggemann B, Gruber C, Wahn U. Perennial allergen sensitisation early in life and chronic asthma in children: a birth cohort study Lancet 2006;368:763-70. (Pubitemid 44279054)
10. Sly PD, Boner AL, Björksten B, Bush A, Custovic A, Eigen-mann PA, et al. Early identification of atopy in the prediction of persistent asthma in children. Lancet 2008;372:1100-6.
11. Borrego LM, Stocks J, Leiria-Pinto P, Peralta I, Romeira AM, Neuparth N, et al. Lung function and clinical risk factors for asthma in infants and young children with recurrent wheeze. Thorax 2009;64:203-9.
12. Matricardi PM, Illi S, Grüber C, Keil T, Nickel R, Wahn U, et al. Wheezing in childhood: incidence, longitudinal patterns and factors predicting persistence. Eur Respir J 2008;32:585-92.
13. Nickel R, Lau S, Niggemann B, Grüber C, von Mutius E, Illi S, et al. The MAS group. Messages from the German multi-centre allergy study Pediatr Allergy Immunol 2002;13(Suppl 15):7-10. (Pubitemid 36125077)
14. Wickman M, Lilja G, Soderstrom L, van Hage-Hamsten M, Ahlstedt S. Quantitative analysis of IgE antibodies to food and inhalant allergens in 4-year-old children reflects their likelihood of allergic disease. Allergy 2005;60:650-7. (Pubitemid 40562472)
15. Szefler SJ, Phillips BR, Martinez FD, Chinchilli VM, Lemanske RF, Strunk RC, et al. Characterization of within-subject responses to fluticasone and montelukast in childhood asthma. J Allergy Clin Immunol 2005;115:233-42. (Pubitemid 40215084)
16. Bousquet J, Jefery PK, Busse WW, Johnson M, Vignola AM. Asthma. From bronchoconstriction to airways inflammation and remodeling. State of the art. Am J Respir Crit Care Med 2000;161:1720-45. (Pubitemid 30329053)
17. Platts-Mills T, Wheatley L. The role of allergy and atopy in asthma. Curr Opin Pulm Med 1996;2:29-34. (Pubitemid 126441409)
18. 2LEN and Inter-Airways document. Allergy 2007;62:457-70. (Pubitemid 46608365)
19. Schildcrout JS, Sheppard L, Lumley T, Slaughter JC, Koenig JQ, Shapiro GG. Ambient air pollution and asthma exacerba-tions in children: an eight-city analysis. Am J Epidemiol 2006; 164:505-17. (Pubitemid 44412779)
20. Locksley RM. Asthma and allergic inflammation. Cell 2010; 140:777-83.
21. Kato A, Schleimer RP. Beyond inflammation: airway epithelial cells are at the interface of innate and adaptive immunity Curr Opin Immunol 2007;19:711-20. (Pubitemid 350236807)
22. Denburg JA, Inman MD, Leber B, Sehmi R, O'Byrne PM. The role of the bone marrow in allergy and asthma. Allergy 1996; 51:141-8. (Pubitemid 26183299)
23. Maret M, Ruffie C, Letuve S, Phelep A, Thibaudeau O, Mar-chal J, et al. A role for bid in eosinophil apoptosis and in aller-gic airway reaction. J Immunol 2009;182:5740-7.
24. Canonica G, Ciprandi G. Adhesion molecules of the allergic inflammation: recent insight on their functional role. Allergy 1994;49:135-41.
25. Busse WW, Lemanske RF. Asthma. N Engl J Med 2001;344: 350-62. (Pubitemid 32107645)
26. Yates DH. Role of exhaled nitric oxide in asthma. Immunol Cell Biol 2001;79:178-90. (Pubitemid 32281090)
27. Tillie-Leblond I, de Blic J, Jaubert F, Wallaert B, Scheinmann P, Gosset P. Airway remodelling is correlated with obstruction in children with severe asthma. Allergy 2008;63:533-41. (Pubitemid 351490406)
28. Detoraki A, Granata F, Staibano S, Rossi FW, Marone G, Geno-vese A. Angiogenesis and lymphangiogenesis in bronchial asth-ma. Allergy 2010;65:946-58.
29. Hogan SP, Rosenberg HF, Moqbel R, Phipps S, Foster PS, Lacy P, et al. Eosinophils: biological properties and role in health and disease. Clin Exp Allergy 2008;38:709-50. (Pubitemid 351550670)
30. Robbinson DS. The role of the mast cell in asthma: induction of airway hyperresponsiveness by interaction with smooth mus-cle? J Allergy Clin Immunol 2004;114:58-65.
31. Bradding P, Walls AF, Holgate ST The role of the mast cell in the pathophysiology of asthma. J Allergy Clin Immunol 2006; 117:1277-84. (Pubitemid 43795741)
32. Monteseirin J. Neutrophils and asthma. J Investig Allergol Clin Immunol 2009;19:340-54.
33. Carlo T, Levy BD. Chemical mediators and the resolution of airway inflammation. Allergol Int 2008;57:299-305.
34. Clarke DL, Dakshinamurti S, Larsson AK, Ward JE, Yamasaki A. Lipid metabolites as regulators of airway smooth muscle function. Pulm Pharmacol Ther 2009;22:426-35.
35. Peters-Golden M. The alveolar macrophage. The forgotten cell in asthma. Am J Respir Cell Mol Biol 2004;31:3-7. (Pubitemid 38888032)
36. Commins SP, Borish L, Steinke JW. Immunologic messenger molecules: cytokines, interferons and chemokines. J Allergy Clin Immunol 2010;2(Suppl 2):S53-72.
37. Afshar R, Medoff BD, Luster AD. Allergic asthma: a tale of many T cells. Clin Exp Allergy 2008;38:1847-57.
38. Palomares O, Yaman G, Azkur AK, Akkoc T, Akdis M, Akdis CA. Role of Treg in immune regulation of allergic diseases. Eur J Immunol 2010;40:1232-40.
39. Kornerup KN, Page CP. The role of platelets in the pathophy-siology of asthma. Platelets 2007;18:319-28.
40. Klinger MH. Platelets and inflammation. Anat Embryol 1997; 196:1-11. (Pubitemid 27332296)
41. Holgate ST The airway epithelium is central to the pathogen-esis of asthma. Allergol Int 2008;57:1-10. (Pubitemid 351413241)
42. Westergren-Thorsson G, Larsen K, Nihlberg K, Andersson-Sjö-land A, Hallgren O, Marko-Varga G, et al. Pathological airway remodelling in inflammation. Clin Respir J 2010;4(Suppl 1): 1-8.
43. Oliver BG, Black JL. Airway smooth muscle and asthma. Allergol Int 2006;55:215-23. (Pubitemid 44433589)
44. Bischof RJ, Bourke JE, Hirst SJ, Meeusen EN, Snibson KJ, Van Der Velden J. Measurement and impact of remodelling in the lung. Airway neovascularization in asthma. Proc Am Thorac Soc 2009;6:673-7.
45. Leung TF, Wong GW, Ko FW, Li CY, Yung E, Lam CW, et al. Analysis of growth factors and inflammatory cytokines in exhaled breath condensate from asthmatic children. Int Arch Allergy Immunol 2005;137:66-72. (Pubitemid 40754724)
46. Barbato A, Turato G, Baraldo S, Bazzan E, Calabrese F, Paniz-zolo C, et al. Epithelial damage and angiogenesis in the airways of children with asthma. Am J Respir Crit Care Med 2006;174: 975-81. (Pubitemid 44684445)
47. Lemanske RF Jr. Inflammation in childhood asthma and other wheezing disorders. Pediatrics 2002;109:368-72. (Pubitemid 34126850)
48. Vignola AM, Bousquet J, Chanez P, Gagliardo R, Merendino AM, Chiappara G, et al. Assessment of airway inflammation in asthma. Am J Crit Care Med 1998;157:184-7.
49. Barnes PJ, Chung KF, Page CP. Inflammatory mediators of asthma: an update. Pharmacol Rev 1998;50:515-96. (Pubitemid 29028812)
50. Brightling CE, Bradding P, Pavord ID, Wardlaw AJ. New insights into the role of the mast cell in asthma. Clin Exp Allergy 2003;33:550-6. (Pubitemid 36629677)
51. Kelly EA, Busse WW Who is captain of the inflammatory ship in asthma? Am J Respir Crit Care Med 2004;169:551-2. (Pubitemid 38270886)
52. Teixeira LK, Fonseca BP, Barbosa BA, Viola JP. The role of interferon-7 on immune and allergic responses. Mem Inst Oswaldo Cruz 2005;100(Suppl I):137-44. (Pubitemid 40685454)
53. Raiden S, Schettini J, Salamone G, Trevani A, Vermeulen M, Gamberale R, et al. Human platelets produce granulocyte-mac-rophage colony-stimulating factor and delay eosinophil apop-tosis. Lab Invest 2003;85:589-98. (Pubitemid 36457713)
54. Bouzigon E, Forabosco P, Koppelman GH, Cookson WO, Dizier M-H, Duffy DL, et al. Meta-analysis of20 genome-wide linkage studies evidenced new regions linked to asthma and atopy. Eur J Hum Genet 2010;18:700-6.
55. Von Mutius E. Gene-environment interactions in asthma. J Allergy Clin Immunol 2009;123:3-11.
56. Dut R, Dizdar EA, Birben E, Sackesen C, Soyer OU, Besler T et al. Oxidative stress and its determinants in the airways of children with asthma. Allergy 2008;63:1605-9.
57. Serham CN. Resolution phase of inflammation: novel endo-genous anti-inflammatory and proresolving lipid mediators and pathways. Annu Rev Immunol 2007;25:101-37.
58. Levy BD, Bonnans C, Silverman ES, Palmer LJ, Marigowda G, Israel E. Diminished lipoxin biosynthesis in severe asthma. Am J Respir Crit Care Med 2005;172:824-30. (Pubitemid 41369064)
59. Guilbert TW, Morgan WJ, Zeiger RS, Mauger DT, Boehmer SJ, Szefler SJ, et al. Long-term inhaled corticosteroids in pre-school children at high risk for asthma. N Engl J Med 2006; 354:1985-97.
60. Tulic MK, Christodoulopoulos P, Hamid Q. Small airway inflammation in asthma. Respir Res 2002;2:333-9. (Pubitemid 34189661)
61. Kharitonov SA. Exhaled markers of inflammatory lung dis-eases: ready for routine monitoring? Swiss Med Wkly 2004; 134:175-92. (Pubitemid 38529381)
62. Kercsmar C Exhaled nitric oxide in the diagnosis and man-agement of childhood asthma. Ther Adv Respir Dis 2010;4: 71-82.
63. de Jongste JC, Carraro S, Hop WC, the CHARISM Study Group, Baraldi E. Daily telemonitoring of exhaled nitric oxide and symptoms in the treatment of childhood asthma. Am J Res-pir Crit Care Med 2009;179:93-7.
64. Murphy AW, Platts-Mills TA, Lobo M, Hayden F. Respiratory nitric oxide levels in experimental human infuenza. Chest 1998;114:452-6. (Pubitemid 28387522)
65. Olin AC, Aldenbratt A, Ekman A, Ljungkvist G, Jungersten L, Alving K, et al. Increased nitric oxide in exhaled air after intake of a nitrate-rich meal. Respir Med 2001;95:153-8. (Pubitemid 32624447)
66. Henriksen AH, Sue-Chu M, Lingaas HT, Langhammer A, Bjer-mer L. Exhaled and nasal NO levels in allergic rhinitis: relation to sensitization, pollen season and bronchial hyperresponsive-ness. Eur Respir J 1999;13:301-6. (Pubitemid 29086824)
67. Arkless Gray K. Exhaled nitric oxide related to allergic con-ditions. Pediatr Pulmonol 2008;43:1198-205.
68. Terada A, Fujisawa T, Togashi K, Miyazaki T, Katsumata H, Atsuta J, et al. Exhaled nitric oxide decreases during exercise-induced bronchoconstriction in children with asthma. Am J Respir Crit Care Med 2001;164:1879-84. (Pubitemid 33152324)
69. Montuschi P, editor. New perspectives in monitoring lung infla-mation. Analysis of exhaled breath condensate. London, New York, Washington, Boca Raton: CRC Press, 2004.
70. Cepelak I, Dodig S. Exhaled breath condensate: a new method for lung disease diagnosis. Clin Chem Lab Med 2007; 45: 945-52. (Pubitemid 47430558)
71. Carraro S, Baraldi E. Exhaled breath condensate in children: present knowledge and future prospects. J Breath Res 2008;2. doi: 10.1088/1752-7155/2/3/ 037003.
72. Cepelak I, Dodig S. Noninvasive methods for diagnosing res-piratory inflammations: exhaled breath condensate. Biochemia Medica 2005; 15: 57-63.
73. Horváth I, Hunt J, Barnes PJ, Alving K, Antczak A, Baraldi E, et al., On behalf of the ATS/ERS Task Force on Exhaled Breath Condensate. Exhaled breath condensate: methodological rec-ommendations and unresolved questions. Eur Respir J 2005;26: 523-48.
74. Effros RM, Dunning MB III, Biller J, Shaker R. The promise and perils of exhaled breath condensates. Am J Physiol Lung Cell Mol Physiol 2004;287:1073-80.
75. Remes S, Korppi M, Remes K, Savolainen K, Mononen I, Pek-kanen J. Serum eosinophil cationic protein (ECP) and eosino-phil protein X (EPX) in childhood asthma: the influence of atopy Pediatr Pulmonol 1998;25:167-74. (Pubitemid 28163300)
76. Galez D, Dodig S, Raos M, Nogalo B. C-reactive protein in children with asthma and allergic rhinitis. Biochemia Medica 2006;16:163-9. (Pubitemid 46020201)
77. Dodig S, Raos M, Zrinski Topić R. Eosinophilia in children with atopic eczema/dermatitis syndrome. Paed Croat 2010;54: 59-63.
78. Takemura M, Matsumoto H, Niimi A, Ueda T, Matsuoka H, Yamaguchi M, et al. High sensitivity C-reactive protein in asth-ma. Eur Respir J 2006;27:908-12. (Pubitemid 44911102)
79. Navratil M, Plavec D, Dodig S, Jelcić Z, Nogalo B, Erceg D, et al. Markers of systemic and lung inflammation in childhood asthma. J Asthma 2009;46:822-8.
80. Henderson AJ. Bronchoalveolar lavage in children. Arch Dis Child 1994;70:167-9.
81. Krawiec ME, Westcott JY, Chu HW, Balzar S, Trudeau JB, Schwartz LB, et al. Persistent wheezing in very young children is associated with lower respiratory inflammation. Am J Respir Crit Care Med 2001;163:1338-43. (Pubitemid 32506596)
82. Marguet C, Jouen-Boedes F, Dean TP, Warner JO. Bronchoal-veolar cell profiles in children with asthma, infantile wheeze, chronic cough, or cystic fibrosis. Am J Respir Crit Care Med 1999;159:1533-40. (Pubitemid 29227865)
83. Pabst R, Tschernig T Lymphocyte dynamics: caution in inter-preting BAL numbers. Thorax 1997;52:1078-80. (Pubitemid 27515789)
84. Bentley AM, Meng Q, Robinson DS, Hamid Q, Kay B, Durham SR. Increases in activated T lymphocytes, eosinophils, and cytokine mRNA expression for interleukin-5 and granulocyte/macrophage colony-stimulating factor in bronchial biopsies after allergen inhalation challenge in atopic asthmatics. Am J Respir Cell Mol Biol 1993;8:35-42.
85. Payne DN, Qiu Y, Zhu J, Peachey L, Scallan M, Bush A, et al. Airway inflammation in children with difficult asthma: relationships with airflow limitation and persistent symptoms. Thorax 2004;59:862-9. (Pubitemid 39389626)
86. Ennis M. Do neutrophils contribute to asthma pathophysio-logy? Curr Allergy Asthma Rep 2003;3:159-65.
87. Le Bourgeois M, Goncalves M, Le Clainche L, Benoist M-R, Fournet J-C, Scheinmann P, et al. Bronchoalveolar cells in children < 3 years old with severe recurrent wheezing. Chest 2002; 122: 791-7.
88. Monteseirin J, Vega A, Chacon P, Camacho MJ, El Bekay R, Asturias J, et al. Neutrophils as a novel sourse of eosinophil cationic protein in IgE-mediated processes. J Immunol 2007; 179:2634-41.
89. De Blic J, Tillie-Leblond I, Tonnel AB, Jaubert F, Scheinmann P, Gosset P. Difficult asthma in children: an analysis of airway inflammation. J Allergy Clin Immunol 2004;113;94-100. (Pubitemid 38096167)
90. Koh GC, Shek LP, Goh DY, Van Bever H, Koh DS. Eosino-phil cationic protein: is it useful in asthma? A systematic review Respir Med 2007;101:696-705. (Pubitemid 46335585)
91. Wood LG, Gibson PG, Garg ML. Biomarkers of lipid peroxi-dation, airway inflammation and asthma. Eur Respir J 2003; 21:177-86. (Pubitemid 36150317)
92. Pohunek P, Warner JO, Turzikova J, Kudrmann J, Roche WR. Markers of eosinophilic inflammation and tissue re-modelling in children before clinically diagnosed bronchial asthma. Pediatr Allergy Immunol 2005;1:43-51. (Pubitemid 40313963)
93. McNamara PS, Kicic A, Sutanto EN, Stevens PT, Stick SM. Comparison of techniques for obtaining lower airway epithe-lial cells from children. Eur Respir J 2008;32:763-8.
94. Wilson N, Bridge P, Spanevello A, Silverman M. Induced sputum in children: feasibility, repeatability, and relation of findings to asthma severity Thorax 2000;55:768-74. (Pubitemid 30660905)
95. Gibson PG, Henry RL, Thomas P. Noninvasive assessment of airway inflammation in children: induced sputum, exhaled nitric oxide, and breath condensate. Eur Respir J 2000;16: 1008-15. (Pubitemid 32049395)
96. Green RH, Brightling CE, McKenna S, Hargadon B, Parker D, Bradding P, et al. Asthma exacerbations and sputum eosin-ophil counts: a randomised controlled trial. Lancet 2002; 360:1715-21. (Pubitemid 35447900)
97. Covar RA, Spahn JD, Martin RJ, Silkoff PE, Sundstrom DA, Murphy J, et al. Safety and application of induced sputum analysis in childhood asthma. J Allergy Clin Immunol 2004; 114:575-82. (Pubitemid 39194931)
98. Fahy JV, Boushey HA, Lazarus SC, Mauger EA, Cherniack RM, Chinchilli VM, et al. Safety and reproducibility of spu-tum induction in asthmatic subjects in multicenter study Am Respir Crit Care Med 2001;163:1470-5. (Pubitemid 32506618)
99. Battaglia S, den Hertog H, Timmers MC, Lazeromos SP, Vignola AM, Rabe KF, et al. Small airways function and molecular markers in exhaled air in mild asthma. Thorax 2005;60:639-44. (Pubitemid 41129061)
100. Taylor DR. An eye to the future: exhaled nitric oxide as a predictor of clinical outcomes in asthma. Eur Respir J 2010;35:1200-2.
101. Pijnenburg MW, Bakker EM, Lever S, Hop WC, de Jongste JC High fractional concentration of nitric oxide in exhaled air despite steroid treatment in asthmatic children. Clin Exp Allergy 2005;37:920-5. (Pubitemid 41032541)
102. De Jongste JC. Yes to NO: the first studies on exhaled nitric oxide-driven asthma treatment Eur Respir J 2005;26:379-81.
103. Beck-Ripp J, Griese M, Arenz S, Koring C, Pasqualoni B, Buffer P. Changes of exhaled nitric oxide during steroid treat-ment of childhood asthma. Eur Respir J 2002;19:1015-9. (Pubitemid 34649612)
104. Baraldi E, de Jongste JC Measurement of exhaled nitric oxide in children, 2001. ERS/ATS statement. Eur Respir J 2002; 20:223-37. (Pubitemid 34822946)
105. Malmberg LP, Pelkonen AS, Haahtela T, Turpeinen M. Exhaled nitric oxide rather than lung function distinguishes preschool children with probable asthma. Thorax 2003;58: 494-9. (Pubitemid 36714010)
106. Warke TJ, Fitch PS, Brown V Exhaled nitric oxide correlates with airway eosinophils in childhood asthma. Thorax 2002; 57:383-7. (Pubitemid 34494145)
107. Smith AD, Cowan JO, Filsell S, McLachlan C, Monti-Shee-han G, Jackson P, et al. Diagnosing asthma-comparisons between exhaled nitric oxide measurements and conventional tests. Am J Respir Crit Care Med 2004;169:473-8. (Pubitemid 38187119)
108. Laoudi Y, Nikasinovic L, Sahraoui F, Grimfeld A, Momas I, Just J. Passive smoking is a major determinant of exhaled nitric oxide levels in allergic asthmatic children. Allergy 2010;65:491-7.
109. Strunk RC, Szefler SJ, Phillips BR, Zeiger RS, Chinchilli VM, Larsen G, et al., For the Childhood Asthma Research and Education Network of the National Heart, Lung, and Blood Institute. Relationship of exhaled nitric oxide to clinical and inflammatory markers of persistent asthma in children. J Allergy Clin Immunol 2003;112:883-92. (Pubitemid 37412055)
110. Papaioannou AI, Minas M, Tanou K, Gourgoulianis KI, Kos-tikas K. Exhaled NO may predict loss of asthma control: the effect of concomitant allergic rhinitis. Eur Respir J 2009; 34:1006-7.
111. Pijnenburg MW, Hofhuis W, Hop WC, de Jongste JC Exhaled nitric oxide predicts asthma relapse in children with clinical asthma remission. Thorax 2005;60:215-8. (Pubitemid 40380280)
112. Bisgaard H, Zielen S, Garcia-Garcia ML, Johnston SL, Gilles L, Menten J, et al. Montelukast reduces asthma exacerbations in 2-to 5-year-old children with intermittent asthma. Am J Respir Crit Care Med 2005;171:315-22. (Pubitemid 40223397)
113. Carlsen KH, Anderson SD, Bjermer L, Bonini S, Brusasco V Canonica W, et al. Exercise-induced asthma, respiratory and allergic disorders in elite athletes: epidemiology, mechanisms and diagnosis: part I of the report from the Joint Task Force of the European Respiratory Society (ERS) and the European Academy of Allergy and Clinical Immunology (EAACI) in cooperation with GA2LEN. Allergy 2008;63:387-403. (Pubitemid 351341832)
114. Hunt JF, Fang K, Malik R, Snyder A, Malhotran N, Platts-Mills TA, et al. Endogenous airway acidification: implications for asthma pathophysiology Am J Respir Crit Care Med 2000;161:694-9. (Pubitemid 30171718)
115. Montuschi P, Mondino C, Koch P, Barnes PJ, Ciabattoni G Effects of a leukotriene receptor antagonist on exhaled leu-kotriene E4 and prostanoids in children with asthma. J Allergy Clin Immunol 2006;118:347-53. (Pubitemid 44160715)
116. Robroeks CM, Kant KD, van de Jobsis Q, Hendriks HJ, van Gent R, Wouters EF, et al. Exhaled nitric oxide and biomark-ers in exhaled breath condensate indicate the presence, sever-ity and control of childhood asthma. Clin Exp Allergy 2007; 37:1303-11. (Pubitemid 47372295)
117. Vlasić Z, Dodig S, Cepelak I, Zrinski Topić R, Zivcić J, Noga-lo B, et al. Iron and ferritin concentrations in exhaled breath condensate of children with asthma. J Asthma 2009; 46: 81-5.
118. Barreto M, Villa MP, Olita C, Martella S, Ciabattoni G, Mon-tuschi P. 8-Isoprostane in exhaled breath condensate and exer-cise-induced bronchoconstriction in asthmatic children and adolescents. Chest 2009;135:66-73.
119. Formanek W, Inci D, Lauener RP, Wildhaber JH, Frey U, Hall GL. Elevated nitrite in breath condensates of children with respiratory disease. Eur Respir J 2002;19:487-91. (Pubitemid 34256964)
120. Corradi M, Folesani G, Andreoli R, Manini P, Bodini A, Pia-centini G, et al. Aldehydes and glutathione in exhaled breath condensate of children with asthma exacerbation. Am J Respir Crit Care Med 2003;167:395-9. (Pubitemid 36216422)
121. Ricciardolo FL, Gaston B, Hunt J. Acid stress in the pathology of asthma. J Allergy Clin Immunol 2004;113:610-9. (Pubitemid 38530413)
122. Zhao JJ, Shimizu Y, Dobashi K, Kawata T, Ono A, Yanagitani N, et al. The relationship between oxidative stress and acid stress in adult patients with mild asthma. J Investig Allergol Clin Immunol 2008;18:41-5. (Pubitemid 351340675)
123. Vaughan J. Ngamtrakulpanit L, Pajewski TN, Turner R, Nguyen TA, Smith A, et al. Exhaled breath condensate pH is a robust and reproducible assay of airway acidity Eur Respir J 2003;22:889-94. (Pubitemid 37540637)
124. Poena V, De Amici M, Ouaglini S, Bellaviti G, Castellazi AM, Marseglia G, et al. Asthma biomarkers. Serum eosinophilic cation protein: is there any role in respiratory disorders? J Asthma 2010;47:131-4.
125. Hays SR, Woodruff PG, Khashayar R, Ferrando RE, Liu J, Fung P, et al. Allergen challenge causes inflammation but not goblet cell degranulation in asthmatic subjects. J Allergy Clin Immunol 2001;108:784-90. (Pubitemid 33096211)
126. Weller PF, Tsai M, Galli SJ. Eosinophils, basophils and mast cells. In: Handin RI, Lux SE, Stossel TP, editors. Blood. Prin-ciples and practice of hematology, 2. izdanje. Philadelphia: Lippincott Williams & Wilkins, 2003:569-88.
127. Malm-Erjefält M, Greiff L, Ankerst J, Andersson M, Wallen-gren J, Cardell LO, et al. Circulating eosinophils in asthma, allergic rhinitis, and atopic dermatitis lack morphologi-cal signs of degranulation. Clin Exp Allergy 2005;35:1334-40.
128. Prehn A, Seger RA, Faber J, Torresani T, Molinari L, Gerber A, et al. The relationship of serum-eosinophil cationic protein and eosinophil count to disease activity in children with bron-chial asthma. Pediatr Allergy Immunol 1998;9:197-203. (Pubitemid 29027396)
129. Ahlstrom-Emanuelsson CA, Greiff L, Andersson M, Persson CG, Erjefalt JS. Eosinophil degranulation status in allergic rhinitis: observations before and during seasonal allergen exposure. Eur Respir J 2004;24:750-7. (Pubitemid 39504427)
130. Ahlstedt S, Peterson CG, Enander I. Update in allergy testing in childhood asthma: how do you know whether you are suc-cessfully controlling the patient's inflammation? Ped Pulmo-nol 1995;19(Suppl 11):32-3.
131. Wolthers OD, Heuck C Circadian variations in serum eosin-ophil cationic protein, and serum and urine eosinophil protein X. Pediatr Allergy Immunol 2003;14:130-3. (Pubitemid 36644355)
132. Rubira N, Rodrigo MJ, Pena M, Nogueiras C, Cruz MJ, Cada-hia A. Blood sample processing effect on eosinophil cationic protein concentration. Ann Allergy Asthma Immunol 1997; 78:394-8. (Pubitemid 27175058)
133. Jousilahti P, Salomaa V, Hakala K, Rasi V, Vahtera E, Palosuo T The association of sensitive systemic inflammation markers with bronchial asthma. Ann Allergy Asthma Immunol 2002; 89:381-5. (Pubitemid 36188009)
134. Guidi GC, Salvagno GL. Referenceintervals as atool fortotal quality management. Biochemia Medica 2010;20:165-72.
135. Siroux V, Oryszczyn M-P, Patty E, Kauffmann F, Pison C, Vervloet D, et al. Relationships of allergic sensitization, total immunoglobulin E and blood eosinophils to asthma severity in children of the EGEA study Clin Exp Allergy 2003;33: 746-51 (Pubitemid 36760504)
136. Kovac K, Dodig S, Tjesić-Drinković D, Raos M. Correlation between asthma severity and serum IgE in asthmatic children sensitized to Dermatophagoides pteronyssinus. Arch Med Res 2007;38:99-105. (Pubitemid 44894618)
137. Leung TF, Wong GW, Ko FW, Lam CW, Fok TF. Clinical and atopic parameters and airway inflammatory markers in child-hood asthma: a factor analysis. Thorax 2005;60:822-6. (Pubitemid 41475208)
138. Piacentini GL, Martinati L, Mingoni S, Boner AL. Influence of allergen avoidance on the eosinophil phase of airway inflammation in children with allergic asthma. J Allergy Clin Immunol 1996;97:1079-84. (Pubitemid 26154206)
139. Larson K. Monitoring airway remodelling in asthma. Clin Respir J 2010;4(Suppl 1):35-40.
140. Koller DV. Sampling methods. Urine/blood analysis. Am J Respir Crit Care Med 2000;162:531-3.