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Volumn 27, Issue 4, 2011, Pages 1463-1471

Spatially addressable chemoselective C-terminal ligation of an intein fusion protein from a complex mixture to a hydrazine-terminated surface

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY PURIFICATION; ASSOCIATED COSTS; BIOLOGICAL CHARACTERIZATION; C-TERMINUS; CELL LYSATES; CHEMOSELECTIVE; CLINICAL DIAGNOSTICS; COMPLEX MIXTURE; FUSION PROTEINS; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; PROTEIN RESISTANT SURFACES; RECOGNITION ELEMENT; SITE-SPECIFIC; SOFT LITHOGRAPHY;

EID: 79952913698     PISSN: 07437463     EISSN: 15205827     Source Type: Journal    
DOI: 10.1021/la104186n     Document Type: Article
Times cited : (5)

References (72)
  • 29
    • 79952926532 scopus 로고    scopus 로고
    • EDC/NHS coupling of the amine-terminated azide to the-COOH groups on the surface resulted in a lower yield 63% than EDC/NHS coupling of the amine-terminated alkyne to the surface-COOH groups 86% during hydrazine surface fabrication because of the size difference between the two molecules. The smaller amine-terminated alkyne encounters less hindered access to the surface, resulting in a higher reaction yield
    • EDC/NHS coupling of the amine-terminated azide to the-COOH groups on the surface resulted in a lower yield (63%) than EDC/NHS coupling of the amine-terminated alkyne to the surface-COOH groups (86%) during hydrazine surface fabrication because of the size difference between the two molecules. The smaller amine-terminated alkyne encounters less hindered access to the surface, resulting in a higher reaction yield.
  • 30
    • 79952935020 scopus 로고    scopus 로고
    • Our experiments suggested that under the conditions used here DTT does not significantly affect the SAM on our functionalized chips
    • Our experiments suggested that under the conditions used here DTT does not significantly affect the SAM on our functionalized chips.
  • 32
    • 79952913877 scopus 로고    scopus 로고
    • If significant hydrolysis for both of the thioesters had occurred, there would be no protein binding to the surface, as observed for the native Trx sample, and thus no observed SPR signal
    • If significant hydrolysis for both of the thioesters had occurred, there would be no protein binding to the surface, as observed for the native Trx sample, and thus no observed SPR signal.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.