Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing

Bioorganic and Medicinal Chemistry Letters

Volumn 15, Issue 10, 2005, Pages 2447-2451

  Girish, Aparna ^a   Sun, Hongyan ^a   Yeo, Dawn S Y ^a   Chen, Grace Y J ^a   Chua, Teck Khiang ^a   Yao, Shao Q ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Intein; N terminal cysteine containing protein; Protein array; Proteomics

Indexed keywords

CYSTEINE; FUNGAL PROTEIN; INTEIN; THIOESTER;

AMINO TERMINAL SEQUENCE; ARTICLE; EXPRESSION VECTOR; HOST; PROTEIN AGGREGATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE; SURFACE PROPERTY; YEAST;

BACTERIA (MICROORGANISMS);

EID: 18144374551     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.03.079     Document Type: Article

References (22)

1. G.Y.J. Chen, M. Uttamchandani, R.Y.P. Lue, M.L. Lessaicherre, and S.Q. Yao Curr. Top. Med. Chem. 3 2003 705 724
2. M. Uttamchandani, D.P. Walsh, S.Q. Yao, and Y.T. Chang Curr. Opin. Chem. Biol. 9 2005 4 13
3. H. Zhu Science 293 2001 2101 2105
4. For a recent review, see: D.S.Y. Yeo, R.C. Panicker, L.P. Tan, and S.Q. Yao Comb. Chem. High Throughput Screening 7 2004 213 221
5. C.D. Hodneland, Y.S. Lee, D.H. Min, and M. Mrksich Proc. Natl. Acad. Sci. U.S.A. 99 2002 5048 5052
6. N. Kindermann, M. George, N. Johnsson, and K. Johnsson J. Am. Chem. Soc. 125 2003 7810 7811
7. J. Yin, F. Liu, X. Li, and C.T. Walsh J. Am. Chem. Soc. 126 2004 7754 7755
8. J.A. Camarero, Y. Kwon, and M.A. Coleman J. Am. Chem. Soc. 126 2004 14730 14731
9. M.L. Lesaicherre, R.Y.P. Lue, G.Y.J. Chen, Q. Zhu, and S.Q. Yao J. Am. Chem. Soc. 124 2002 8768 8769
10. R.Y.P. Lue, G.Y.J. Chen, Y. Hu, Q. Zhu, and S.Q. Yao J. Am. Chem. Soc. 126 2004 1055 1062
11. L.P. Tan, R.Y.P. Lue, G.Y.J. Chen, and S.Q. Yao Bioorg. Med. Chem. Lett. 14 2004 6067 6070
12. L.P. Tan, G.Y.J. Chen, and S.Q. Yao Bioorg. Med. Chem. Lett. 14 2004 5735 5738
13. G.Y.J. Chen, M. Uttamchandani, Q. Zhu, G. Wang, and S.Q. Yao ChemBioChem. 4 2003 336 339
14. M. Uttamchandani, E.W.S. Chan, G.Y.J. Chen, and S.Q. Yao Bioorg. Med. Chem. Lett. 13 2003 2997 3000
15. M.L. Lesaicherre, M. Uttamchandani, G.Y.J. Chen, and S.Q. Yao Bioorg. Med. Chem. Lett. 12 2002 2079 2083
16. M.L. Lesaicherre, M. Uttamchandani, G.Y.J. Chen, and S.Q. Yao Bioorg. Med. Chem. Lett. 12 2002 2085 2088
17. S.Y.D. Yeo, R. Srinivasan, M. Uttamchandani, G.Y.J. Chen, Q. Zhu, and S.Q. Yao Chem. Commun. 2003 2870 2871
18. S.Y.D. Yeo, R. Srinivasan, G.Y.J. Chen, and S.Q. Yao Chem. Eur. J. 10 2004 4664 4672
19. 5
20. P.E. Dawson, T.W. Muir, I. Clark-Lewis, and S.B.H. Kent Science 266 1994 776 779
21. For microarray spotting with crude cell lysates expressing a desired N-terminal cysteine-containing protein, bacterial cells were harvested after 18 h of protein expression, lysed in 1 X PBS (pH 7.4) and spotted immediately onto thioester slides
22. 9