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Volumn 68, Issue 3, 1996, Pages 490-497

A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance

Author keywords

[No Author keywords available]

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; HISTIDINE; PROTEIN;

EID: 0030077272     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac9504023     Document Type: Article
Times cited : (473)

References (35)
  • 16
    • 85033812774 scopus 로고    scopus 로고
    • The refractive indices of proteins usually fall between 1.35 and 1.55. The effect of error in the assumed refrective index on the calculated thickness is described in detail in ref 18
    • The refractive indices of proteins usually fall between 1.35 and 1.55. The effect of error in the assumed refrective index on the calculated thickness is described in detail in ref 18.
  • 21
    • 0041742046 scopus 로고
    • Corrections were applied by the Surface Science Instruments software according to: Scofield, J H. J. Electron Spectrosc. 1976, 8, 129-137.
    • (1976) J. Electron Spectrosc. , vol.8 , pp. 129-137
    • Scofield, J.H.1
  • 22
    • 5844372499 scopus 로고
    • 15SH phase segregate into microdomains with length scales of approximately 1-10 nm: Stranick, S. J.; Parikh, A. N.; Tao, Y.-T.; Allara, D. L.; Weiss, P. S. J. Phys. Chem 1994, 98, 7636-7646. Disorder in the tri(ethylene glycol) chains attached to thiols 2 and 3 could lead to better mixing of these thiols; however, the process of phase separation in SAMs is not yet understood well enough to make predictions with confidence.
    • (1994) Phys. Chem , vol.98 , pp. 7636-7646
    • Stranick, S.J.1    Parikh, A.N.2    Tao, Y.-T.3    Allara, D.L.4    Weiss, P.S.J.5
  • 24
    • 85033807036 scopus 로고    scopus 로고
    • note
    • Adsorbed proteins should not be densely packed as long as the NTA-terminated thiolates are randomly distributed or phase segregated into protein size microdomains (see ref 22). Another possibility that we cannot rule out is that the thiolates phase segregated into domains much larger than a protein. This possibility could lead to islands of densely packed proteins surrounded by areas free of protein.
  • 25
    • 0003808259 scopus 로고
    • North-Holland: New York
    • The theoretical SPR response to changes in the index of refraction of the bulk liquid and to deposition of thin protein films was determined by calculating the reflection of p-polarized light from a stratified, planar, isotropic structure, as described by: Azzam, R. M. A.; Bashara, N. M. Ellipsometry and Polarized Light; North-Holland: New York, 1977 The model used consisted of three layers with finite thicknesses (gold, SAM, and protein) between two semiinfinite media (glass and solution). The indices of refraction for the gold (0.17 + 4.931), glass (1.511), and water (1329) layers were taken from ref 5. We used an index of refraction of 1.45 for both the SAM and protein layers (see ref 16).
    • (1977) Ellipsometry and Polarized Light
    • Azzam, R.M.A.1    Bashara, N.M.2
  • 26
    • 85033817832 scopus 로고    scopus 로고
    • note
    • Some possible explanations for the heterogeneity in the dissociation of adsorbed scTCR by imidazole include the following: (i) some fraction of adsorbed protein may have bound to multiple Ni(II) ions and been less sensitive to treatment with imidazole, (ii) there may have been a strongly adsorbing impurity in the scTCR preparation, or (iii) some denaturation of protein may have occurred on the surface
  • 27
    • 85033806970 scopus 로고    scopus 로고
    • This procedure is commonly used to immobilize proteins on the carboxydextran-modified substrates sold by Pharmacia for use with the BIACore SPR instrument (see ref 3 for details)
    • This procedure is commonly used to immobilize proteins on the carboxydextran-modified substrates sold by Pharmacia for use with the BIACore SPR instrument (see ref 3 for details).
  • 29
    • 85033805658 scopus 로고    scopus 로고
    • note
    • -7 M), the surface can be used to quantitate certain protein-protein interactions accurately. More experience with the method is, however, required to evaluate its generality.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.