Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis

Methods

Volumn 53, Issue 4, 2011, Pages 437-452

  Hofemeister, Helmut ^a   Ciotta, Giovanni ^a   Fu, Jun ^a   Seibert, Philipp Martin ^a   Schulz, Alexander ^a   Maresca, Marcello ^a   Sarov, Mihail ^b   Anastassiadis, Konstantinos ^c   Stewart, A Francis ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^c CENTER FOR REGENERATIVE THERAPIES DRESDEN   (Germany)

Author keywords

BAC transgenesis; Chromatin immunoprecipitation; Gene targeting; Genotyping by long range PCR; Native affinity purification; Protein tagging; Recombineering; Western blotting

Indexed keywords

COMPLEMENTARY DNA; MICRORNA; PROTEIN TAG; TRANSCRIPTION FACTOR;

ANIMAL EXPERIMENT; ARTICLE; CELL CYCLE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; EXON; GENE TARGETING; INTRON; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REGULATORY MECHANISM; TRANSGENICS;

ANIMALS; BLOTTING, WESTERN; CHROMATIN IMMUNOPRECIPITATION; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLONING, MOLECULAR; EMBRYONIC STEM CELLS; EPITOPES; GENOTYPE; HUMANS; IMMUNOPRECIPITATION; MASS SPECTROMETRY; MICE; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TRANSGENES;

EID: 79952708432     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.12.026     Document Type: Article

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