Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes

Nature Biotechnology

Volumn 21, Issue 4, 2003, Pages 447-451

  Yang, Yi ^a   Seed, Brian ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; CELLS; ESCHERICHIA COLI; GENETIC ENGINEERING;

STEM CELLS;

GENES;

ANIMAL CELL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CHROMOSOME MUTATION; CONTROLLED STUDY; EMBRYO; ESCHERICHIA COLI; FLUORESCENCE IN SITU HYBRIDIZATION; GENE CONSTRUCT; GENE DISRUPTION; GENE TARGETING; GENETIC RECOMBINATION; GENETIC TRANSDUCTION; HOMOZYGOSITY; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE TARGETING; IN SITU HYBRIDIZATION, FLUORESCENCE; MICE; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; STEM CELLS; TRANSDUCTION, GENETIC;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; RODENTIA;

EID: 0037382904     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt803     Document Type: Article

References (19)

1. Tsuzuki, T. & Rancourt, D.E. Embryonic stem cell gene targeting using bacteriophage lambda vectors generated by phage-plasmid recombination. Nucleic Acids Res. 26, 988-993 (1998).
2. Yang, X.W., Model, P. & Heintz, N. Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nat. Biotechnol. 15, 859-865 (1997).
3. Yu, D. et al. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97, 5978-5983 (2000).
4. Zhang, Y., Buchholz, F., Muyrers, J.P. & Stewart, A.F. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20, 123-128 (1998).
5. Zhang, P., Li, M.Z. & Elledge, S.J. Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step. Nat. Genet. 30, 31-39 (2002).
6. Murphy, K.C. Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol. 180, 2063-2071 (1998).
7. Angrand, P.O., Daigle, N., van der Hoeven, F., Scholer, H.R. & Stewart, A.F. Simplified generation of targeting constructs using ET recombination. Nucleic Acids Res. 27, e16 (1999).
8. Koller, B.H. & Smithies, O. Altering genes in animals by gene targeting. Annu. Rev. Immunol. 10, 705-730 (1992).
9. Soriano, P. Gene targeting in ES cells. Annu. Rev. Neurosci. 18, 1-18 (1995).
10. Deng, C. & Capecchi, M.R. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol. Cell Biol. 12, 3365-3371 (1992).
11. Hasty, P., Rivera-Perez, J. & Bradley, A. The length of homology required for gene targeting in embryonic stem cells. MoL. Cell Biol. 11, 5586-5591 (1991).
12. Poteete, A.R. & Fenton, A.C. Lambda red-dependent growth and recombination of phage P22. Virology 134, 161-167 (1984).
13. El Karoui, M., Amundsen, S.K., Dabert, P. & Gruss, A. Gene replacement with linear DNA in electroporated wild-type EscherichIa coli. Nucleic Acids Res. 27, 1296-1299 (1999).
14. Deichmann, M., Bentz, M. & Haas, R. Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection. J. Virol. Methods 65, 19-25 (1997).
15. Reid, L.H., Shesely, E.G., Kim, H.S. & Smithies, O. Cotransformation and gene targeting in mouse embryonic stem cells. Mol. Cell Biol. 11, 2769-2777 (1991).
16. Bollag, R.J., Waldman, A.S. & Liskay, R.M. Homologous recombination in mammalian cells. Annu. Rev. Genet. 23, 199-225 (1989).
17. Capecchi, M.R. Altering the genome by homologous recombination. Science 244, 1288-1292 (1989).
18. Thyagarajan, B., McCormick-Graham, M., Romero, D.P. & Campbell, C. Characterization of homologous DNA recombination activity in normal and immortal mammalian cells. Nucleic Acids Res. 24, 4084-4091 (1996).
19. Yang, Y. et al. Targeted disruption of the murine Fanconi anemia gene, Fancg/Xrcc9. Blood98, 3435-3440 (2001).