Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells

Cytotechnology

Volumn 51, Issue 2, 2006, Pages 81-88

  Brown, A C ^a   Lerner, C P ^a   Graber, J H ^a   Shaffer, D J ^a   Roopenian, D C ^a  

^a JACKSON LABORATORY   (United States)

Author keywords

Embryonic stem cells; Gene targeting; Homologous recombination; Polymerase chain reaction

Indexed keywords

DNA;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL CULTURE; CELL SCREENING; CONTROLLED STUDY; DNA MODIFICATION; EMBRYO; EMBRYONIC STEM CELL; GENE POOL; GENE TARGETING; GENETIC ENGINEERING; GENETIC SCREENING; GERM LINE; HOMOLOGOUS RECOMBINATION; NONHUMAN; POLYMERASE CHAIN REACTION; REACTION ANALYSIS; SENSITIVITY ANALYSIS; TARGET CELL;

MAMMALIA;

EID: 33751079885     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-006-9021-8     Document Type: Article

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