High-throughput fluorescence assay for small-molecule inhibitors of Autophagins/Atg4

Journal of Biomolecular Screening

Volumn 16, Issue 2, 2011, Pages 174-182

  Shu, Chih Wen ^a   Madiraju, Charitha ^a   Dayong Zhai, ^a   Welsh, Kate ^a   Diaz, Paul ^a   Sergienko, Eduard ^a   Sano, Renata ^a   Reed, John C ^a  

^a BURNHAM INSTITUTE   (United States)

Author keywords

Atg4; autophagin; autophagy; high throughput screening; protease

Indexed keywords

AUTOPHAGIN 1; AUTOPHAGY PROTEIN 4B; CASPASE 3; CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR; HYBRID PROTEIN; N ETHYLMALEIMIDE; PHOSPHOLIPASE A2; UNCLASSIFIED DRUG;

ARTICLE; AUTOPHAGY; CELL ORGANELLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; MOLECULAR LIBRARY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING;

AUTOPHAGY; CASPASE 3; CYSTEINE ENDOPEPTIDASES; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; GENE ORDER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; RECOMBINANT FUSION PROTEINS; SMALL MOLECULE LIBRARIES; SPECTROMETRY, FLUORESCENCE;

EID: 79951968329     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110392996     Document Type: Article

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