An In Vitro model that recapitulates the epithelial to Mesenchymal transition (EMT) in Human Breast Cancer

PLoS ONE

Volumn 6, Issue 2, 2011, Pages

  Katz, Elad ^a   Dubois Marshall, Sylvie ^a   Sims, Andrew H ^a   Gautier, Philippe ^b   Caldwell, Helen ^a   Meehan, Richard R ^a,b   Harrison, David J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CLAUDIN; COLLAGEN; NERVE CELL ADHESION MOLECULE; UVOMORULIN; VIMENTIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CANCER INVASION; CELL FUNCTION; CELL STRUCTURE; CONTROLLED STUDY; DOWN REGULATION; EPITHELIAL MESENCHYMAL TRANSITION; GENE EXPRESSION PROFILING; GENE IDENTIFICATION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RAT; RNA SEQUENCE; SEQUENCE ANALYSIS; STROMA CELL; THREE DIMENSIONAL IMAGING; AMINO ACID SEQUENCE; BASEMENT MEMBRANE; BIOLOGICAL MODEL; BREAST TUMOR; CHEMISTRY; FEMALE; GENETICS; METABOLISM; MOLECULAR GENETICS; PATHOLOGY; TUMOR CELL LINE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BASEMENT MEMBRANE; BREAST NEOPLASMS; CELL LINE, TUMOR; CLAUDINS; COLLAGEN; EPITHELIAL-MESENCHYMAL TRANSITION; FEMALE; HUMANS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; NEOPLASM INVASIVENESS; STROMAL CELLS; TRANSCRIPTION FACTORS;

EID: 79951871973     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017083     Document Type: Article

References (42)

1. Fernandez Y, Cueva J, Palomo AG, Ramos M, de Juan A, et al. (2010) Novel therapeutic approaches to the treatment of metastatic breast cancer. Cancer Treat Rev 36: 33-42.
2. Arendt LM, Rudnick JA, Keller PJ, Kuperwasser C, (2010) Stroma in breast development and disease. Semin Cell Dev Biol 21: 11-18.
3. Weigelt B, Peterse JL, van 't Veer LJ, (2005) Breast cancer metastasis: markers and models. Nat Rev Cancer 5: 591-602.
4. Thiery JP, (2003) Epithelial-mesenchymal transitions in development and pathologies. Curr Opin Cell Biol 15: 740-746.
5. Thiery JP, Sleeman JP, (2006) Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol 7: 131-142.
6. Polyak K, Weinberg RA, (2009) Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer 9: 265-273.
7. Huber MA, Kraut N, Beug H, (2005) Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol 17: 548-558.
8. Gupta PB, Onder TT, Jiang G, Tao K, Kuperwasser C, et al. (2009) Identification of selective inhibitors of cancer stem cells by high-throughput screening. Cell 138: 645-659.
9. Schmalhofer O, Brabletz S, Brabletz T, (2009) E-cadherin, beta-catenin, and ZEB1 in malignant progression of cancer. Cancer Metastasis Rev 28: 151-166.
10. Hazan RB, Qiao R, Keren R, Badano I, Suyama K, (2004) Cadherin switch in tumor progression. Ann N Y Acad Sci 1014: 155-163.
11. Peinado H, Olmeda D, Cano A, (2007) Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat Rev Cancer 7: 415-428.
12. Hazan RB, Phillips GR, Qiao RF, Norton L, Aaronson SA, (2000) Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis. J Cell Biol 148: 779-790.
13. Nieman MT, Prudoff RS, Johnson KR, Wheelock MJ, (1999) N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. J Cell Biol 147: 631-644.
14. Herschkowitz JI, Simin K, Weigman VJ, Mikaelian I, Usary J, et al. (2007) Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol 8: R76.
15. Tomaskovic-Crook E, Thompson EW, Thiery JP, (2009) Epithelial to mesenchymal transition and breast cancer. Breast Cancer Res 11: 213.
16. Evans EE, Henn AD, Jonason A, Paris MJ, Schiffhauer LM, et al. (2006) C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer. Mol Cancer Ther 5: 2919-2930.
17. Katz E, Dubois-Marshall S, Sims AH, Faratian D, Li J, et al. (2010) A gene on the HER2 amplicon, C35, is an oncogene in breast cancer whose actions are prevented by inhibition of Syk. Br J Cancer 103: 401-410.
18. Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, et al. (2004) Bioconductor: open software development for computational biology and bioinformatics. Genome Biol 5: R80.
19. Dunning MJ, Smith ML, Ritchie ME, Tavare S, (2007) beadarray: R classes and methods for Illumina bead-based data. Bioinformatics 23: 2183-2184.
20. Tusher VG, Tibshirani R, Chu G, (2001) Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A 98: 5116-5121.
21. van Helden J, Andre B, Collado-Vides J, (1998) Extracting regulatory sites from the upstream region of yeast genes by computational analysis of oligonucleotide frequencies. J Mol Biol 281: 827-842.
22. Thomas-Chollier M, Sand O, Turatsinze JV, Janky R, Defrance M, et al. (2008) RSAT: regulatory sequence analysis tools. Nucleic Acids Res 36: W119-127.
23. Matys V, Kel-Margoulis OV, Fricke E, Liebich I, Land S, et al. (2006) TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes. Nucleic Acids Res 34: D108-110.
24. Neve RM, Chin K, Fridlyand J, Yeh J, Baehner FL, et al. (2006) A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer Cell 10: 515-527.
25. Bailey CM, Khalkhali-Ellis Z, Kondo S, Margaryan NV, Seftor RE, et al. (2005) Mammary Serine Protease Inhibitor (Maspin) Binds Directly to Interferon Regulatory Factor 6: Identification of a Novel Serpin Partnership. J Biol Chem 280: 34210-34217.
26. Zhou C, Nitschke AM, Xiong W, Zhang Q, Tang Y, et al. (2008) Proteomic analysis of tumor necrosis factor-alpha resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype. Breast Cancer Res 10: R105.
27. Prat A, Parker JS, Karginova O, Fan C, Livasy C, et al. (2010) Phenotypic and molecular characterization of the claudin-low intrinsic subtype of breast cancer. Breast Cancer Res 12: R68.
28. Parr C, Jiang WG, (2006) Hepatocyte growth factor activation inhibitors (HAI-1 and HAI-2) regulate HGF-induced invasion of human breast cancer cells. Int J Cancer 119: 1176-1183.
29. Fanayan S, Shehata M, Agterof AP, McGuckin MA, Alonso MA, et al. (2009) Mucin 1 (MUC1) is a novel partner for MAL2 in breast carcinoma cells. BMC Cell Biol 10: 7.
30. Steed E, Rodrigues NT, Balda MS, Matter K, (2009) Identification of MarvelD3 as a tight junction-associated transmembrane protein of the occludin family. BMC Cell Biol 10: 95.
31. Ellison G, Klinowska T, Westwood RF, Docter E, French T, et al. (2002) Further evidence to support the melanocytic origin of MDA-MB-435. Mol Pathol 55: 294-299.
32. Debnath J, Brugge JS, (2005) Modelling glandular epithelial cancers in three-dimensional cultures. Nat Rev Cancer 5: 675-688.
33. Kuperwasser C, Dessain S, Bierbaum BE, Garnet D, Sperandio K, et al. (2005) A mouse model of human breast cancer metastasis to human bone. Cancer Res 65: 6130-6138.
34. Flanagan L, Van Weelden K, Ammerman C, Ethier SP, Welsh J, (2000) SUM-159PT cells: a novel estrogen independent human breast cancer model system. Breast Cancer Res Treat 58: 193-204.
35. Gaggioli C, Hooper S, Hidalgo-Carcedo C, Grosse R, Marshall JF, et al. (2007) Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells. Nat Cell Biol 9: 1392-1400.
36. Taube JH, Herschkowitz JI, Komurov K, Zhou AY, Gupta S, et al. (2010) Core epithelial-to-mesenchymal transition interactome gene-expression signature is associated with claudin-low and metaplastic breast cancer subtypes. Proc Natl Acad Sci U S A 107: 15449-15454.
37. Yilmaz M, Christofori G, (2010) Mechanisms of motility in metastasizing cells. Mol Cancer Res 8: 629-642.
38. Sabeh F, Shimizu-Hirota R, Weiss SJ, (2009) Protease-dependent versus-independent cancer cell invasion programs: three-dimensional amoeboid movement revisited. J Cell Biol 185: 11-19.
39. Wiercinska E, Naber HP, Pardali E, van der Pluijm G, van Dam H, et al. (2010) The TGF-beta/Smad pathway induces breast cancer cell invasion through the up-regulation of matrix metalloproteinase 2 and 9 in a spheroid invasion model system. Breast Cancer Res Treat [Epub ahead of print].
40. Yook JI, Li XY, Ota I, Hu C, Kim HS, et al. (2006) A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells. Nat Cell Biol 8: 1398-1406.
41. Hotary K, Li XY, Allen E, Stevens SL, Weiss SJ, (2006) A cancer cell metalloprotease triad regulates the basement membrane transmigration program. Genes Dev 20: 2673-2686.
42. Klymkowsky MW, Savagner P, (2009) Epithelial-mesenchymal transition: a cancer researcher's conceptual friend and foe. Am J Pathol 174: 1588-1593.