Directed evolution of an aminoalcohol dehydrogenase for efficient production of double chiral aminoalcohols

Journal of Bioscience and Bioengineering

Volumn 111, Issue 3, 2011, Pages 266-271

  Urano, Nobuyuki ^a   Fukui, Satoko ^a   Kumashiro, Shoko ^a   Ishige, Takeru ^a   Kita, Shinji ^b   Sakamoto, Keiji ^b   Kataoka, Michihiko ^a,c   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Daiichi Fine Chemical Co Ltd   (Japan)

^c Osaka Prefecture University   (Japan)

Author keywords

Amino acid substitution; Aminoalcohol dehydrogenase; Directed enzyme evolution; Enzyme screening; Short chain alcohol dehydrogenase

Indexed keywords

AMINO ACID SUBSTITUTION; AMINO ALCOHOLS; DIRECTED ENZYME EVOLUTION; DIRECTED EVOLUTION; EFFICIENT PRODUCTION; ENZYME-SUBSTRATE BINDING; HIGH CONCENTRATION; HIGH-THROUGHPUT SCREENING; MUTANT ENZYMES; MUTANT LIBRARIES; PARAMETER ANALYSIS; PSEUDOEPHEDRINE; RANDOM MUTATION; REACTION MIXTURE; RHODOCOCCUS ERYTHROPOLIS; SHORT-CHAIN ALCOHOL DEHYDROGENASE; STEREOSELECTIVE REDUCTION; TRANSFORMANT CELLS; WEAK POINTS; WILD TYPES; WILD-TYPE ENZYMES;

AMINO ACIDS; CATALYSTS; ENZYME ACTIVITY; ESCHERICHIA COLI;

ENZYMES;

ALCOHOL DEHYDROGENASE; AMINOALCOHOL; AMINOALCOHOL DEHYDROGENASE; PSEUDOEPHEDRINE; UNCLASSIFIED DRUG;

ALCOHOL PRODUCTION; AMINO ACID SUBSTITUTION; ARTICLE; CHIRALITY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; ESCHERICHIA COLI; EVOLUTION; GENE MUTATION; HIGH THROUGHPUT SCREENING; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RHODOCOCCUS ERYTHROPOLIS;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; AMINO ALCOHOLS; BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; DNA, BACTERIAL; ESCHERICHIA COLI; MUTAGENESIS; PROTEIN BINDING; RHODOCOCCUS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; RHODOCOCCUS ERYTHROPOLIS;

EID: 79951813407     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.11.005     Document Type: Article

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