Forensic biochemistry for functional investigation of death: Concept and practical application

Legal Medicine

Volumn 13, Issue 2, 2011, Pages 55-67

  Maeda, Hitoshi ^a,b   Ishikawa, Takaki ^a,b   Michiue, Tomomi ^a,b  

^a OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b Medico legal Consultation   (Japan)

Author keywords

Cause of death; Death process; Forensic biochemistry; Laboratory investigation; Trauma; Unexpected sudden death

Indexed keywords

BIOLOGICAL MARKER;

ANAPHYLAXIS; ANEMIA; ASPHYXIA; AUTOPSY; BLEEDING; BRAIN DAMAGE; CAUSE OF DEATH; CHEMICAL ANALYSIS; CHRONIC DISEASE; COLD EXPOSURE; CONGESTIVE HEART FAILURE; DEHYDRATION; DIABETES MELLITUS; DROWNING; ELECTROCUTION; ELECTROLYTE DISTURBANCE; FATALITY; FIRE; FORENSIC MEDICINE; HEART DISEASE; HEAT STROKE; HUMAN; HYPOTHERMIA; HYPOXEMIA; HYPOXIA; INFECTION; INFLAMMATION; INJURY; INTOXICATION; KIDNEY FAILURE; LABORATORY TEST; LUNG INJURY; LUNG INSUFFICIENCY; MEDICOLEGAL ASPECT; METABOLIC DISORDER; MUSCLE INJURY; PATHOPHYSIOLOGY; QUANTITATIVE ANALYSIS; REVIEW; RISK ASSESSMENT; STARVATION; STRESS;

AUTOPSY; BIOCHEMISTRY; BIOLOGICAL MARKERS; DEATH, SUDDEN; FORENSIC PATHOLOGY; HUMANS; MOLECULAR BIOLOGY; POSTMORTEM CHANGES;

EID: 79251607725     PISSN: 13446223     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.legalmed.2010.12.005     Document Type: Review

References (172)

1. Saukko P., Knight B. The forensic autopsy. Knight's forensic pathology, 3rd ed. 2004, 1-51. Hodder Arnold, London. P. Saukko, B. Knight (Eds.).
2. Cohle S.D., Sampson B.A. The negative autopsy: sudden cardiac death or other?. Cardiovasc Pathol 2001, 10:219-222.
3. Basso C., Burke M., Fornes P., Gallagher P.J., de Gouveia R.H., Sheppard M., et al. Guidelines for autopsy investigation of sudden cardiac death. Virchows Arch 2008, 452:11-18. [Association for European Cardiovascular Pathology].
4. Coe J.I. Postmortem chemistry of blood, cerebrospinal fluid, and vitreous humor. Forensic medicine, vol 2 1977, 1030-1060. WB Sauders, Philadelphia. C.G. Tedeschi, W.C. Eckert, L.G. Tedeschi (Eds.).
5. Coe J.I. Postmortem chemistry update. Emphasis on forensic application. Am J Forensic Med Pathol 1993, 14:91-117.
6. Friedrich G. Forensische postmortale Biochemie. In: Forster B (hrsg.), Praxis der Rechtsmedizin fuer Mediziner und Juristen. 1986, p. 789-831.
7. Maeda H. Pathophysiochemistry of acute death: an approach to evidence-based assessment in forensic pathology. Nihon Hoigaku Zasshi 2004, 58:121-129.
8. Maeda H., Zhu B.L., Ishikawa T., Quan L., Michiue T. Significance of postmortem biochemistry in determining the cause of death. Leg Med (Tokyo) 2009, 11:S46-S49.
9. Madea B. Sudden death, especially in infancy-improvement of diagnoses by biochemistry, immunohistochemistry and molecular pathology. Leg Med (Tokyo) 2009, 11:S36-S42.
10. Madea B., Musshoff F. Postmortem biochemistry. Forensic Sci Int 2007, 165:165-171.
11. Luna A. Is postmortem biochemistry really useful? Why is it not widely used in forensic pathology?. Leg Med (Tokyo) 2009, 11:S27-S30.
12. Kernbach-Wighton G. Diagnostic problems with functional causes of death: analytical approaches and procedures. Leg Med (Tokyo) 2009, 11:S31-S35.
13. Morris J.A., Harrison L.M., Partridge S.M. Postmortem bacteriology: a re-evaluation. J Clin Pathol 2006, 59:1-9.
14. Zhu B.L., Ishikawa T., Michiue T., Quan L., Maeda H. Postmortem serum endotoxin level in relation to the causes of death. Leg Med (Tokyo) 2005, 7:103-109.
15. Dirnhofer R., Jackowski C., Vock P., Potter K., Thali M.J. VIRTOPSY: minimally invasive, imaging-guided virtual autopsy. Radiographics 2006, 26:1305-1333.
16. Christe A., Flach P., Ross S., Spendlove D., Bolliger S., Vock P., et al. Clinical radiology and postmortem imaging (Virtopsy) are not the same: specific and unspecific postmortem signs. Leg Med (Tokyo) 2010, 12:215-222.
17. Michiue T., Ishikawa T., Sakoda S., Quan L., Li D.R., Kamikodai Y., et al. Cardiothoracic ratio in postmortem chest radiography with regard to the cause of death. Leg Med (Tokyo) 2010, 12:73-78.
18. Schneider P.M. Scientific standards for studies in forensic genetics. Forensic Sci Int 2007, 165:238-243.
19. Morling N. PCR in forensic genetics. Biochem Soc Trans 2009, 37(Pt 2):438-440.
20. Tester D.J., Ackerman M.J. The role of molecular autopsy in unexplained sudden cardiac death. Curr Opin Cardiol 2006, 21:166-172.
21. de la Grandmaison G.L. Is there progress in the autopsy diagnosis of sudden unexpected death in adults?. Forensic Sci Int 2006, 156:138-144.
22. Karch S.B. Changing times: DNA resequencing and the nearly normal autopsy. J Forensic Leg Med 2007, 14:389-397.
23. Zumwalt R.E. Application of molecular biology to forensic pathology. Hum Pathol 1989, 20:303-307.
24. Tu E., Bagnall R.D., Duflou J., Lynch M., Twigg S.M., Semsarian C. Post-mortem pathologic and genetic studies in " dead in bed syndrome" cases in type 1 diabetes mellitus. Hum Pathol 2010, 41:392-400.
25. Bauer M. RNA in Forensic Science. Forensic Sci Int Genet 2007, 1:69-74.
26. Zhao D., Ishikawa T., Quan L., Michiue T., Zhu B.L., Maeda H. Postmortem quantitative mRNA analyses of death investigation in forensic pathology: an overview and prospects. Leg Med (Tokyo) 2009, 11:S43-S45.
27. Maeda H., Zhu B.L., Ishikawa T., Michiue T. Forensic molecular pathology of violent deaths. Forensic Sci Int 2010, 203:83-92.
28. Oxford B.S., Dovenbarger S. Specimen collection and processing. Clinical Chemistry, 3rd ed 1996, 39-60. Lippincott-Raven, Philadelphia. M.L. Bishop, J.L. Duben-Engelkirk, E.P. Fody (Eds.).
29. Cembrowski G.S., Sullivan A.M. Quality control and statistics. Clinical Chemistry, 3rd ed 1996, 61-96. Lippincott-Raven, Philadelphia. M.L. Bishop, J.L. Duben-Engelkirk, E.P. Fody (Eds.).
30. Quan L., Ishikawa T., Michiue T., Li D.R., Zhao D., Yoshida C., et al. Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. Leg Med (Tokyo) 2009, 11:S297-S300.
31. Fujita M.Q., Zhu B.L., Ishida K., Quan L., Oritani S., Maeda H. Serum C-reactive protein levels in postmortem blood - an analysis with special reference to the cause of death and survival time. Forensic Sci Int 2002, 130:160-166.
32. Ishikawa T., Hamel M., Zhu B.L., Li D.R., Zhao D., Michiue T., et al. Comparative evaluation of postmortem serum concentrations of neopterin and C-reactive protein. Forensic Sci Int 2008, 179:135-143.
33. Zhu B.L., Ishida K., Quan L., Taniguchi M., Oritani S., Li D.R., et al. Postmortem serum uric acid and creatinine levels in relation to the causes of death. Forensic Sci Int 2002, 125:59-66.
34. Zhu B.L., Ishikawa T., Michiue T., Tanaka S., Zhao D., Li D.R., et al. Differences in postmortem urea nitrogen, creatinine and uric acid levels between blood and pericardial fluid in acute death. Leg Med (Tokyo) 2007, 9:115-122.
35. Maeda H., Zhu B.L., Bessho Y., Ishikawa T., Quan L., Michiue T., et al. Postmortem serum nitrogen compounds and C-reactive protein levels with special regard to investigation of fatal hyperthermia. Forensic Sci Med Pathol 2008, 4:175-180.
36. Maeda H., Michiue T., Zhu B.L., Ishikawa T., Quan L., Bessho Y., et al. Potential risk factors for sudden cardiac death: an analysis of medicolegal autopsy cases. Leg Med (Tokyo) 2009, 11:S263-S265.
37. Zhu B.L., Ishida K., Taniguchi M., Quan L., Oritani S., Tsuda K., et al. Possible postmortem serum markers for differentiation between fresh-, saltwater drowning and acute cardiac death: a preliminary investigation. Leg Med (Tokyo) 2003, 5:S298-S301.
38. Maeda H., Zhu B.L., Ishikawa T., Quan L., Michiue T., Bessyo Y., et al. Analysis of postmortem biochemical findings with regard to the lung weight in drowning. Leg Med (Tokyo) 2009, 11:S269-S272.
39. Zhu B.L., Ishida K., Quan L., Taniguchi M., Oritani S., Kamikodai Y., et al. Post-mortem urinary myoglobin levels with reference to the causes of death. Forensic Sci Int 2001, 115:183-188.
40. Quan L., Zhu B.L., Ishikawa T., Michiue T., Zhao D., Li D.R., et al. Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy. Int J Leg Med 2008, 122:481-487.
41. Quan T., Zhu B.L., Ishikawa T., Michiue T., Zhao D., Ogawa D., et al. Postmortem serum erythropoietin level as a marker of survival time in injury deaths. Forensic Sci Int 2010, 200:117-122.
42. Tsokos M., Reichelt U., Jung R., Nierhaus A., Püschel K. Interleukin-6 and C-reactive protein serum levels in sepsis-related fatalities during the early postmortem period. Forensic Sci Int 2001, 119:47-56.
43. Mimasaka S., Ohtsu Y., Tsunenari S., Funayama M. Postmortem cytokine levels and severity of traumatic injuries. Int J Leg Med 2006, 120:265-270.
44. Reichelt U., Jung R., Nierhaus A., Tsokos M. Serial monitoring of interleukin-1beta, soluble interleukin-2 receptor and lipopolysaccharide binding protein levels after death A comparative evaluation of potential postmortem markers of sepsis. Int J Leg Med 2005, 119:80-87.
45. Jashnani K.D., Kale S.A., Rupani A.B. Vitreous humor: biochemical constituents in estimation of postmortem interval. J Forensic Sci 2010, 55:1523-1527.
46. Mulla A., Massey K.L., Kalra J. Vitreous humor biochemical constituents: evaluation of between-eye differences. Am J Forensic Med Pathol 2005, 26:146-149.
47. Zhu B.L., Quan L., Li D.R., Zhao D., Maeda H. Evaluation of postmortem serum calcium and magnesium levels in relation to the causes of death in forensic autopsy. Forensic Sci Int 2005, 155:18-23.
48. Li D.R., Quan L., Zhu B.L., Ishikawa T., Michiue T., Zhao D., et al. Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy. Leg Med (Tokyo) 2009, 11:S276-S278.
49. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Quan L., et al. Evaluation of postmortem urea nitrogen, creatinine and uric acid levels in pericardial fluid in forensic autopsy. Leg Med (Tokyo) 2005, 7:287-292.
50. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Bessho Y., et al. Postmortem cardiac troponin I and creatine kinase MB levels in the blood and pericardial fluid as markers of myocardial damage in medicolegal autopsy. Leg Med (Tokyo) 2007, 9:241-250.
51. Maeda H., Michiue T., Zhu B.L., Ishikawa T., Quan L. Analysis of cardiac troponins and creatine kinase MB in cerebrospinal fluid in medicolegal autopsy cases. Leg Med (Tokyo) 2009, 11:S266-S268.
52. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Oritani S., et al. Postmortem cardiac troponin T levels in the blood and pericardial fluid. Part 1. Analysis with special regard to traumatic causes of death. Leg Med (Tokyo) 2006, 8:86-93.
53. Maeda H., Fukita K., Oritani S., Nagai K., Zhu B.L. Evaluation of postmortem oxymetry in fire victims. Forensic Sci Int 1996, 81:201-209.
54. Maeda H., Fukita K., Oritani S., Ishida K. Evaluation of post-mortem oxymetry with reference to the causes of death. Forensic Sci Int 1997, 87:201-210.
55. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Quan L., et al. Postmortem serum catecholamine levels in relation to the cause of death. Forensic Sci Int 2007, 173:122-129.
56. Ishikawa T., Yoshida C., Michiue T., Perdekamp M.G., Pollak S., Maeda H. Immunohistochemistry of catecholamines in the hypothalamic-pituitary-adrenal system with special regard to fatal hypothermia and hyperthermia. Leg Med (Tokyo) 2010, 12:121-127.
57. Quan L, Ishikawa T, Hara J, Michiue T, Chen JH, Wang Q, Zhu BL, Maeda H. Postmortem serotonin levels in cerebrospinal and pericardial fluids with regard to the cause of death in medicolegal autopsy. Leg Med (Tokyo) 2010; [Epub ahead of print].
58. Isbister G.K., Buckley N.A., Whyte I.M. Serotonin toxicity: a practical approach to diagnosis and treatment. Med J Aust 2007, 187:361-365.
59. Pilgrim JL, Gerostamoulos D, Drummer OH. Review: Pharmacogenetic aspects of the effect of cytochrome P450 polymorphisms on serotonergic drug metabolism, response, interactions, and adverse effects. Forensic Sci Med Pathol 2010; [Epub ahead of print].
60. Yoshida C., Ishikawa T., Michiue T., Zhao D., Komatsu A., Quan L., et al. Immunohistochemical distribution of chromogranin A in medicolegal autopsy materials. Leg Med (Tokyo) 2009, 11:S231-S233.
61. Yoshida C., Ishikawa T., Michiue T., Quan L., Maeda H. Postmortem biochemistry and immunohistochemistry of chromogranin A as a stress marker with special regard to fatal hypothermia and hyperthermia. Int J Leg Med 2011, 125:11-20.
62. Ishikawa T., Quan Li, Li D.R., Zhao D., Michiue T., Hamel M., et al. Postmortem biochemistry and immunohistochemistry of adrenocorticotropic hormone with special regard to fatal hypothermia. Forensic Sci Int 2008, 179:147-151.
63. Ishikawa T., Michiue T., Quan L., Zhao D., Komatsu A., Bessho Y., et al. Morphological and functional alterations in the adenohypophysis in cases of brain death. Leg Med (Tokyo) 2009, 11:S234-S237.
64. Ishikawa T., Michiue T., Zhao D., Komatsu A., Azuma Y., Quan L., et al. Evaluation of postmortem serum and cerebrospinal fluid levels of thyroid-stimulating hormone with special regard to fatal hypothermia. Leg Med (Tokyo) 2009, 11:S228-S230.
65. Quan L., Zhu B.L., Ishida K., Oritani S., Taniguchi M., Fujita M.Q., et al. Intranuclear ubiquitin immunoreactivity of the pigmented neurons of the substantia nigra in fatal acute mechanical asphyxiation and drowning. Int J Leg Med 2001, 115:6-11.
66. Quan L., Zhu B.L., Oritani S., Ishida K., Fujita M.Q., Maeda H. Intranuclear ubiquitin immunoreactivity in the pigmented neurons of the substantia nigra in fire fatalities. Int J Leg Med 2001, 114:310-315.
67. Quan L., Ishikawa T., Michiue T., Li D.R., Zhao D., Zhu B.L., et al. Quantitative morphometry of granular 'dot-like' ubiquitin-immunoreactivity in the crus cerebri in asphyxiation and fire fatalities. Leg Med (Tokyo) 2005, 7:81-88.
68. Quan L., Ishikawa T., Michiue T., Li D.R., Zhao D., Oritani S., et al. Ubiquitin-immunoreactive structures in the midbrain of methamphetamine abusers. Leg Med (Tokyo) 2005, 7:144-150.
69. Quan L., Ishikawa T., Michiue T., Li D.R., Zhao D., Zhu B.L., et al. Quantitative analysis of ubiquitin-immunoreactivity in the midbrain periaqueductal gray matter with regard to the causes of death in forensic autopsy. Leg Med (Tokyo) 2005, 7:151-156.
70. Quan L., Ishikawa T., Michiue T., Li D.R., Zhao D., Zhu B.L., et al. Ubiquitin immunoreactivity in the midbrain as a marker of stress to motor nervous systems in fatal injury. Leg Med (Tokyo) 2005, 7:157-163.
71. Kock K.F., Simonsen K.W. Renal myoglobin in drug addicts: occurrence and significance in a postmortem study. Forensic Sci Int 1994, 65:113-119.
72. Ishikawa T., Zhu B.L., Li D.R., Zhao D., Michiue T., Maeda H. Immunohistochemical investigation of ubiquitin and myoglobin in the kidney in medicolegal autopsy cases. Forensic Sci Int 2007, 171:136-141.
73. Hougen H.P., Valenzuela A., Lachica E., Villanueva E. Sudden cardiac death: a comparative study of morphological, histochemical and biochemical methods. Forensic Sci Int 1992, 52:161-169.
74. Osuna E., Pérez-Cárceles M.D., Alvarez M.V., Noguera J., Luna A. Cardiac troponin I (cTn I) and the postmortem diagnosis of myocardial infarction. Int J Leg Med 1998, 111:173-176.
75. Cina S.J., Li D.J., Chan D.W., Boitnott J.K., Hruban R.H., Smialek J.E. Serum concentrations of cardiac troponin I in sudden death: a pilot study. Am J Forensic Med Pathol 1998, 19:324-328.
76. Cina S.J., Thompson W.C., Fischer J.R., Brown D.K., Titus J.M., Smialek J.E. A study of various morphologic variables and troponin I in pericardial fluid as possible discriminators of sudden cardiac death. Am J Forensic Med Pathol 1999, 20:333-337.
77. Perez-Carceles M.D., Noguera J., Jimenez J.L., Martinez P., Luna A., Osuna E. Diagnostic efficacy of biochemical markers in diagnosis postmortem of ischemic heart disease. Forensic Sci Int 2004, 142:1-7.
78. Torres C., Jarreta B.M., Alegret R., Hernandez del Rincón J.P., Falcon M., Gómez Zapata M., et al. Analysis of ionic ratios in the interventricular wall and their relation with cardiac damage as seen in anatomo-pathological and cardiac biomarkers. Leg Med (Tokyo) 2009, 11:S360-S362.
79. Peter J., Kirchner A., Kuhlisch E., Menschikowski M., Neef B., Dressler J. The relevance of the detection of troponins to the forensic diagnosis of cardiac contusion. Forensic Sci Int 2006, 160:127-133.
80. Martínez Díaz F., Rodríguez-Morlensín M., Pérez-Cárceles M.D., Noguera J., Luna A., Osuna E. Biochemical analysis and immunohistochemical determination of cardiac troponin for the postmortem diagnosis of myocardial damage. Histol Histopathol 2005, 20:475-481.
81. Davies S.J., Gaze D.C., Collinson P.O. Investigation of cardiac troponins in postmortem subjects: comparing antemortem and postmortem levels. Am J Forensic Med Pathol 2005, 26:213-215.
82. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Kamikodai Y., et al. Postmortem cardiac troponin T levels in the blood and pericardial fluid. Part 2. Analysis for application in the diagnosis of sudden cardiac death with regard to pathology. Leg Med (Tokyo) 2006, 8:94-101.
83. Cina S.J., Brown D.K., Smialek J.E., Collins K.A. A rapid postmortem cardiac troponin T assay: laboratory evidence of sudden cardiac death. Am J Forensic Med Pathol 2001, 22:173-176.
84. Ellingsen C.L., Hetland O. Serum concentrations of cardiac troponin T in sudden death. Am J Forensic Med Pathol 2004, 25:213-215.
85. Dressler J., Felscher D., Koch R., Muller E. Troponin T in legal medicine. Lancet 1998, 352:38.
86. Meng X., Ming M., Wang E. Heart fatty acid binding protein as a marker for postmortem detection of early myocardial damage. Forensic Sci Int 2006, 160:11-16.
87. Yasuda T., Iida R., Kawai Y., Nakajima T., Kominato Y., Fujihara J., et al. Serum deoxyribonuclease I can be used as a useful marker for diagnosis of death due to ischemic heart disease. Leg Med (Tokyo) 2009, 11:S213-S215.
88. Osuna E., Perez-Carceles M.D., Luna A., Pounder D.J. Efficacy of cerebro-spinal fluid biochemistry in the diagnosis of brain insult. Forensic Sci Int 1992, 52:193-198.
89. Vázquez M.D., Sánchez-Rodriguez F., Osuna E., Diaz J., Cox D.E., Pérez-Cárceles M.D., et al. Creatine kinase BB and neuron-specific enolase in cerebrospinal fluid in the diagnosis of brain insult. Am J Forensic Med Pathol 1995, 16:210-214.
90. Nogami M., Takatsu A., Ishiyama I. Immunohistochemical study of neuron-specific enolase in human brains from forensic autopsies. Forensic Sci Int 1998, 94:97-109.
91. Li D.R., Zhu B.L., Ishikawa T., Zhao D., Michiue T., Maeda H. Postmortem serum protein S100B levels with regard to the cause of death involving brain damage in medicolegal autopsy cases. Leg Med (Tokyo) 2006, 8:71-77.
92. Li D.R., Michiue T., Zhu B.L., Ishikawa T., Quan L., Zhao D., et al. Evaluation of postmortem S100B levels in the cerebrospinal fluid with regard to the cause of death in medicolegal autopsy. Leg Med (Tokyo) 2009, 11:S273-S275.
93. Li D.R., Ishikawa T., Zhao D., Michiue T., Quan L., Zhu B.L., et al. Histopathological changes of the hippocampus neurons in brain injury. Histol Histopathol 2009, 24:1113-1120.
94. Li D.R., Ishikawa T., Quan L., Zhao D., Michiue T., Zhu B.L., et al. Morphological analysis of astrocytes in the hippocampus in mechanical asphyxiation. Leg Med (Tokyo) 2010, 12:63-67.
95. Li D.R., Zhu B.L., Ishikawa T., Zhao D., Michiue T., Maeda H. Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy. Leg Med (Tokyo) 2006, 8:78-85.
96. Ishida K., Zhu B.L., Quan L., Fujita M.Q., Maeda H. Pulmonary surfactant-associated protein A levels in cadaveric sera with reference to the cause of death. Forensic Sci Int 2000, 109:125-133.
97. Zhu B.L., Ishida K., Quan L., Li D.R., Taniguchi M., Fujita M.Q., et al. Pulmonary immunohistochemistry and serum levels of a surfactant-associated protein A in fatal drowning. Leg Med (Tokyo) 2002, 4:1-6.
98. Maeda H., Fujita M.Q., Zhu B.L., Ishida K., Quan L., Oritani S., et al. Pulmonary surfactant-associated protein A as a marker of respiratory distress in forensic pathology: assessment of the immunohistochemical and biochemical findings. Leg Med (Tokyo) 2003, 5:S318-S321.
99. Quan L., Zhu B.L., Ishikawa T., Michiue T., Zhao D., Yoshida C., et al. Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy. Leg Med (Tokyo) 2009, 11:S301-S303.
100. Zhu B.L., Maeda H., Fukita K., Sakurai M., Kobayashi Y. Immunohistochemical investigation of pulmonary surfactant in perinatal fatalities. Forensic Sci Int 1996, 83:219-227.
101. Zhu B.L., Ishida K., Fujita M.Q., Maeda H. Immunohistochemical investigation of a pulmonary surfactant in fatal mechanical asphyxia. Int J Leg Med 2000, 113:268-271.
102. Zhu B.L., Ishida K., Quan L., Fujita M.Q., Maeda H. Immunohistochemistry of pulmonary surfactant apoprotein A in forensic autopsy: reassessment in relation to the causes of death. Forensic Sci Int 2000, 113:193-197.
103. Zhu B.L., Ishida K., Oritani S., Quan L., Fujita M.Q., Maeda H., et al. Immunohistochemical investigation of pulmonary surfactant-associated protein A in fatal poisoning. Forensic Sci Int 2001, 117:205-212.
104. Zhu B.L., Ishida K., Oritani S., Quan L., Taniguchi M., Li D.R., et al. Immunohistochemical investigation of pulmonary surfactant-associated protein A in fire victims. Leg Med (Tokyo) 2001, 3:23-28.
105. Zhu B.L., Ishida K., Quan L., Fujita M.Q., Maeda H. Immunohistochemistry of pulmonary surfactant-associated protein A in acute respiratory distress syndrome. Leg Med (Tokyo) 2001, 3:134-140.
106. Stemberga V., Stifter S., Cuculić D., Coklo M., Bosnar A. Immunohistochemical surfactant protein-A expression: fatal drowning vs. postmortem immersion. Med Hypotheses 2009, 72:413-415.
107. Pérez-Cárceles M.D., Sibón A., Vizcaya M.A., Osuna E., Gómez-Zapata M., Luna A., et al. Histological findings and immunohistochemical surfactant protein A (SP-A) expression in asphyxia: its application in the diagnosis of drowning. Histol Histopathol 2008, 23:1061-1068.
108. Ishida K., Zhu B.L., Maeda H. Novel approach to quantitative reverse transcription PCR assay of mRNA component in autopsy material using the TaqMan fluorogenic detection system. Forensic Sci Int 2000, 113:127-131.
109. Ishida K., Zhu B.L., Maeda H. A quantitative RT-PCR assay of surfactant-associated protein A1 and A2 mRNA transcripts as a diagnostic tool for acute asphyxial death. Leg Med (Tokyo) 2002, 4:7-12.
110. Ishida K., Zhu B.L., Maeda H. TaqMan fluorogenic detection system to analyze gene transcription in autopsy material. Methods Mol Biol 2005, 291:415-421.
111. Alpert J.S., Thygesen K., Antman E., Bassand J.P. Myocardial infarction redefined-a consensus document of The Joint European Society of Cardiology. J Am Coll Cardiol 2000, 36:959-969. [American College of Cardiology Committee for the redefinition of myocardial infarction].
112. Lorente J.A., Villanueva E., Hernández-Cueto C., Luna J.D. Plasmatic levels of atrial natriuretic peptide (ANP) in drowning. A pilot study. Forensic Sci Int 1990, 44:69-75.
113. Zhu B.L., Ishikawa T., Michiue T., Li D.R., Zhao D., Tanaka S., et al. Postmortem pericardial natriuretic peptides as markers of cardiac function in medico-legal autopsies. Int J Leg Med 2007, 121:28-35.
114. Michaud K., Augsburger M., Donzé N., Sabatasso S., Faouzi M., Bollmann M., et al. Evaluation of postmortem measurement of NT-proBNP as a marker for cardiac function. Int J Leg Med 2008, 122:415-420.
115. Ardizzone N., Cappello F., Di Felice V., Rappa F., Minervini F., Marasà S., et al. Atrial natriuretic peptide and CD34 overexpression in human idiopathic dilated cardiomyopathies. APMIS 2007, 115:1227-1233.
116. Kubo S., Kitamura O., Orihara Y., Ogata M., Tokunaga I., Nakasono I. Immunohistochemical diagnosis and significance of forensic neuropathological changes. J Med Invest 1998, 44:109-119.
117. Kitamura O. Immunohistochemical investigation of hypoxic/ischemic brain damage in forensic autopsy cases. Int J Leg Med 1994, 107:69-76.
118. Michiue T., Ishikawa T., Quan L., Li D.R., Zhao D., Komatsu A., et al. Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain: an analysis of autopsy material with regard to the cause of death. Forensic Sci Int 2008, 178:185-191.
119. Michiue T., Quan L., Ishikawa T., Zhu B.L., Maeda H. Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. Leg Med (Tokyo) 2009, 11:S168-S170.
120. Schwartz L.B., Metcalfe D.D., Miller J.S., Earl H., Sullivan T. Tryptase levels as an indicator of mast-cell activation in systemic anaphylaxis and mastocytosis. N Engl J Med 1987, 316:1622-1626.
121. Ansari M.Q., Zamora J.L., Lipscomb M.F. Postmortem diagnosis of acute anaphylaxis by serum tryptase analysis. Am J Clin Pathol 1993, 99:101-103.
122. Edston E., van Hage-Hamsten M. Beta-tryptase measurements post-mortem in anaphylactic deaths and in controls. Forensic Sci Int 1998, 93:135-142.
123. Nishio H., Suzuki K. Three cases of suspected hyperthermia with remarkable elevation of serum mast cell tryptase. Forensic Sci Int 2005, 149:51-55.
124. Edston E., Eriksson O., van Hage M. Mast cell tryptase in postmortem serum-reference values and confounders. Int J Leg Med 2007, 121:275-280.
125. Nishio H., Takai S., Miyazaki M., Horiuchi H., Osawa M., Uemura K., et al. Usefulness of serum mast cell-specific chymase levels for postmortem diagnosis of anaphylaxis. Int J Leg Med 2005, 119:331-334.
126. Tamaki K., Sato K., Katsumata Y. Enzyme-linked immunosorbent assay for determination of plasma thyroglobulin and its application to post-mortem diagnosis of mechanical asphyxia. Forensic Sci Int 1987, 33:259-265.
127. Tamaki K., Katsumata Y. Enzyme-linked immunoabsorbent assay for plasma thyroglobulin following compression of the neck. Forensic Sci Int 1990, 44:193-201.
128. Müller E., Franke W.G., Koch R. Thyroglobulin and violent asphyxia. Forensic Sci Int 1997, 90:165-170.
129. Dressler J., Mueller E. High thyroglobulin (Tg) concentrations in fatal traumatic brain injuries. Am J Forensic Med Pathol 2006, 27:280-282.
130. Rainio J., De Giorgio F., Bortolotti F., Tagliaro F. Objective post-mortem diagnosis of chronic alcohol abuse - a review of studies on new markers. Leg Med (Tokyo) 2008, 10:229-235.
131. Michiue T., Ishikawa T., Quan L., Zhu B.L., Maeda H. Forensic pathological evaluation of injury severity and fatal outcome in traffic accidents: five illustrative autopsy cases of clinically unexpected death. Forensic Sci Med Pathol 2008, 4:153-158.
132. Oehmichen M., Meissner C., Schmidt V., Pedal I., König H.G., Saternus K.S. Axonal injury-a diagnostic tool in forensic neuropathology? A review. Forensic Sci Int 1998, 95:67-83.
133. Geddes J.F., Vowles G.H., Beer T.W., Ellison D.W. The diagnosis of diffuse axonal injury: implications for forensic practice. Neuropathol Appl Neurobiol 1997, 23:339-347.
134. Chen J.H., Quan L., Ishikawa T., Michiue T., Wang Q., Zhu B.L., et al. Postmortem lung weight with regard to survival time. Leg Med (Tokyo) 2009, 11:S238-S240.
135. Quan L., Fujita M.Q., Zhu B.L., Ishida K., Maeda H. Immunohistochemical distribution of C-reactive protein in the hepatic tissue in forensic autopsy. Forensic Sci Int 2000, 113:177-182.
136. Tsokos M., Fehlauer F., Püschel K. Immunohistochemical expression of E-selectin in sepsis-induced lung injury. Int J Leg Med 2000, 113:338-342.
137. An J.L., Ishida Y., Kimura A., Tsokos M., Kondo T. Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury. Forensic Sci Int 2009, 192:e21-e25.
138. Tsokos M. Postmortem diagnosis of sepsis. Forensic Sci Int 2007, 165:155-164.
139. Tsokos M. Immunohistochemical detection of sepsis-induced lung injury in human autopsy material. Leg Med (Tokyo) 2003, 5:73-86.
140. An J.L., Ishida Y., Kimura A., Kondo T. Forensic application of intrarenal aquaporin-2 expression for differential diagnosis between freshwater and saltwater drowning. Int J Leg Med 2010, 124:99-104.
141. Hayashi T., Ishida Y., Mizunuma S., Kimura A., Kondo T. Differential diagnosis between freshwater drowning and saltwater drowning based on intrapulmonary aquaporin-5 expression. Int J Leg Med 2009, 123:7-13.
142. Zhu B.L., Quan L., Li D.R., Taniguchi M., Kamikodai Y., Tsuda K., et al. Postmortem lung weight in drownings: a comparison with acute asphyxiation and cardiac death. Leg Med (Tokyo) 2003, 5:20-26.
143. Zhu B.L., Quan L., Ishida K., Oritani S., Li D.R., Taniguchi M., et al. Lung-heart weight ratio as a possible index of cardiopulmonary pathophysiology in drowning. Leg Med (Tokyo) 2003, 5:S295-S297.
144. Betz P., Nerlich A., Penning R., Eisenmenger W. Alveolar macrophages and the diagnosis of drowning. Forensic Sci Int 1993, 62:217-224.
145. Sugimura T., Kashiwagi M., Matsusue A., Hara K., Kageura M., Kubo S. Application of the drowning index to actual drowning cases. Leg Med (Tokyo) 2010, 12:68-72.
146. Coco T.J., Klasner A.E. Drug-induced rhabdomyolysis. Curr Opin Pediatr 2004, 16:206-210.
147. Khan F.Y. Rhabdomyolysis: a review of the literature. Neth J Med 2009, 67:272-283.
148. Welte T., Bohnert M., Pollak S. Prevalence of rhabdomyolysis in drug deaths. Forensic Sci Int 2004, 139:21-25.
149. Unkrig S., Hagemeier L., Madea B. Postmortem diagnostics of assumed food anaphylaxis in an unexpected death. Forensic Sci Int 2010, 198:e1-e4.
150. Osawa M., Satoh F., Horiuchi H., Tian W., Kugota N., Hasegawa I. Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: evaluation of clinical laboratory parameters and immunohistochemistry in three cases. Leg Med (Tokyo) 2008, 10:143-147.
151. Fineschi V., Riezzo I., Cantatore S., Pomara C., Turillazzi E., Neri M. Complement C3a expression and tryptase degranulation as promising histopathological tests for diagnosing fatal amniotic fluid embolism. Virchows Arch 2009, 454:283-290.
152. Zhu B.L., Ishikawa T., Quan L., Oritani S., Li D.R., Zhao D., et al. Possible factors contributing to the postmortem lung weight in fire fatalities. Leg Med (Tokyo) 2005, 7:139-143.
153. Marschall S., Rothschild M.A., Bohnert M. Expression of heat-shock protein 70 (Hsp70) in the respiratory tract and lungs of fire victims. Int J Leg Med 2006, 120:355-359.
154. Weis A., Bohnert M. Expression patterns of adhesion molecules P-selectin, von Willebrand factor and PECAM-1 in lungs: a comparative study in cases of burn shock and hemorrhagic shock. Forensic Sci Int 2008, 175:102-106.
155. Quan L., Ishikawa T., Zhao D., Michiue T., Yoshida C., Chen J.H., et al. Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. Leg Med (Tokyo) 2009, 11:S294-S296.
156. Teresiński G., Buszewicz G., Madro R. The influence of ethanol on the level of ketone bodies in hypothermia. Forensic Sci Int 2002, 127:88-96.
157. Tsokos M., Rothschild M.A., Madea B., Rie M., Sperhake J.P. Histological and immunohistochemical study of Wischnewsky spots in fatal hypothermia. Am J Forensic Med Pathol 2006, 27:70-74.
158. Preuss J., Dettmeyer R., Poster S., Lignitz E., Madea B. The expression of heat shock protein 70 in kidneys in cases of death due to hypothermia. Forensic Sci Int 2008, 176:248-252.
159. Michiue T., Ishikawa T., Zhao D., Kamikodai Y., Zhu B.L., Maeda H. Pathological and biochemical analysis of the pathophysiology of fatal electrocution in five autopsy cases. Leg Med (Tokyo) 2009, 11:S549-S552.
160. Ishikawa T., Zhu B.L., Miyaishi S., Ishizu H., Maeda H. Histopathological changes of the hypophysis in malnutrition in elderly subjects. Hum Cell 2005, 18:157-162.
161. Mortensen E.S., Rognum T.O., Straume B., Jørgensen L. Frequency of acute asymptomatic myocardial infarction and an estimate of infarct age in cases of abrupt sudden death observed in a forensic autopsy material. J Cell Mol Med 2008, 12:2119-2129.
162. Thomsen H., Held H. Immunohistochemical detection of C5b-9(m) in myocardium: an aid in distinguishing infarction-induced ischemic heart muscle necrosis from other forms of lethal myocardial injury. Forensic Sci Int 1995, 71:87-95.
163. Edston E., Kawa K. Immunohistochemical detection of early myocardial infarction. An evaluation of antibodies against the terminal complement complex (C5b-9). Int J Leg Med 1995, 108:27-30.
164. Campobasso C.P., Dell'Erba A.S., Addante A., Zotti F., Marzullo A., Colonna M.F. Sudden cardiac death and myocardial ischemia indicators: a comparative study of four immunohistochemical markers. Am J Forensic Med Pathol 2008, 29:154-161.
165. Chen X., Zhang Y. Myocardial Cx43 expression in the cases of sudden death due to dilated cardiomyopathy. Forensic Sci Int 2006, 162:170-173.
166. Zhu B.L., Tanaka S., Ishikawa T., Zhao D., Li D.R., Michiue T., et al. Forensic pathological investigation of myocardial hypoxia-inducible factor-1 alpha, erythropoietin and vascular endothelial growth factor in cardiac death. Leg Med (Tokyo) 2008, 10:11-19.
167. Leadbeatter S., Wawman H.M., Jasani B. Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage. Forensic Sci Int 1990, 45:135-141.
168. Kucher N., Goldhaber S.Z. Cardiac biomarkers for risk stratification of patients with acute pulmonary embolism. Circulation 2003, 108:2191-2194.
169. Kernbach-Wighton G., Püschel K. On the phenomenology of lethal applications of insulin. Forensic Sci Int 1998, 93:61-73.
170. Brinkmann B., Fechner G., Karger B., DuChesne A. Ketoacidosis and lactic acidosis - frequent causes of death in chronic alcoholics?. Int J Leg Med 1998, 111:115-119.
171. Teresiński G., Buszewicz G., Madro R. Acetonaemia as an initial criterion of evaluation of a probable cause of sudden death. Leg Med (Tokyo) 2009, 11:18-24.
172. Germerott T., Preiss U.S., Ebert L.C., Ruder T.D., Ross S., Flach P.M., et al. A new approach in virtopsy: postmortem ventilation in multislice computed tomography. Leg Med (Tokyo) 2010, 12:276-279.