The human gut mobile metagenome, a metazoan perspective

Gut Microbes

Volumn 1, Issue 6, 2010, Pages

  Jones, Brian V ^a  

^a UNIVERSITY OF BRIGHTON   (United Kingdom)

Author keywords

Gut microbiota; Holobiont; Hologenome; Horizontal gene transfer; Metagenomics; Mobile genetic elements; Mobile metagenome; Toxin antitoxin addiction module

Indexed keywords

TOXIN;

ARTICLE; BACTERIUM ISOLATION; CONTROLLED STUDY; EUROPE; FIRMICUTES; HUMAN; HUMAN GENOME; INCIDENCE; INTESTINE; JAPAN; METAGENOMICS; METAZOON; MICROBIAL COMMUNITY; MICROBIOME; NONHUMAN; PREVALENCE; UNITED STATES; WESTERN HEMISPHERE; BACTERIUM; COMPARATIVE STUDY; GASTROINTESTINAL TRACT; GENETICS; METAGENOME; MICROBIOLOGY; PLASMID; REVIEW;

BACTERIA (MICROORGANISMS); FIRMICUTES; METAZOA;

BACTERIA; GASTROINTESTINAL TRACT; HUMANS; METAGENOME; PLASMIDS;

EID: 79251559429     PISSN: 19490976     EISSN: 19490984     Source Type: Journal    
DOI: None     Document Type: Article

References (117)

1. Whitman WB, Coleman DC, Wiebe WJ. Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 1998; 95:6578-83.
2. Bäckhed F, Ley RE, Sonnenburg JL, Peterson DA, Gordon JI. Host-bacterial mutualism in the human intestine. Science 2005; 307:1915-20.
3. O'Hara AM, Shanahan F. The gut flora as a forgotten organ. EMBO Rep 2006; 7:688-93.
4. Zilber-Rosenberg I, Rosenberg E. Role of microorganisms in the evolution of animals and plants: the hologenome theory of evolution. FEMS Microbiol Rev 2008; 32:723-5.
5. Rosenberg E, Sharon G, Zilber-Rosenberg I. The hologenome theory of evolution contains Lamarckian aspects within a Darwinian framework. Environ Microbiol 2009; 11:2959-62.
6. Dethlefsen L, McFall-Ngai M, Relman DA. An ecological and evolutionary perspective on human-microbe mutualism and disease. Nature 2007; 449:811-8.
7. Wilks M. Bacteria and early human development. Early Hum Dev 2007; 83:165-70.
8. Ley RE, Peterson DA, Gordon JI. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell 2006; 124:837-48.
9. Turnbaugh PJ, Quince C, Faith JJ, McHardy AC, Yatsunenko T, Niazi F, et al. Organismal, genetic and transcriptional variation in the deeply sequenced gut microbiomes of identical twins. Proc Natl Acad Sci USA 2010; 107:7503-8.
10. Qin J, Li R, Raes J, Arumugaum M, Burgdorf KS, Manichanh C, et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 2010; 464:59-65.
11. Tap J, Mondot S, Levenez F, Pelletier E, Caron C, Furet JP, et al. Towards the human intestinal microbiota phylogenetic core. Environ Microbiol 2009; 11:2574-84.
12. Backhed F, Ding H, Wang T, Hooper LV, Koh Y, Nagy A, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci USA 2004; 101:15718-23.
13. Rawls JF, Mahowald MA, Ley RE, Gordon JI. Reciprocal gut microbiota transplants from zebrafish and mice to germ-free recipients reveal host habitat selection. Cell 2006; 127:423-33.
14. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444:1027-31.
15. Hooper LV, Xu J, Falk PG, Midtvedt T, Gordon JI. A molecular sensor that allows a gut commensal to control its nutrient foundation in a competitive ecosystem. Proc Natl Acad Sci USA 1999; 96:9833-8.
16. Stappenback TS, Hooper LV, Gordon JI. Development regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. Proc Natl Acad Sci USA 2002; 99:15451-5.
17. Zoetendal EG, Vaughan EE, de Vos WM. A microbial world within us. Mol Microbiol 2006; 59:1639-50.
18. Lombardo P. Access to mutualistic endosymbiotic microbes: an underappreciated benefit of group living. Behav Ecol Sociobiol 2008; 62:479-97.
19. Gill SR, Pop M, Deboy RT, Eckburg PB, Turnbaugh PJ, Samuel BS, et al. Metagenomic analysis of the human distal gut microbiome. Science 2006; 312:1355-9.
20. Kurokawa K, Itoh T, Kuwahara T, Oshima K, Toh H, Toyoda A, et al. Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. DNA Res 2007; 14:169-81.
21. Jones BV, Begley M, Hill C, Gahan CGM, Marchesi JR. Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome. Proc Natl Acad Sci USA 2008; 105:13580-5.
22. Jones BV, Sun F, Marchesi JR. Comparative metagenomic analysis of plasmid encoded functions in the human gut microbiome. BMC Genomics 2010; 11:46.
23. Jones BV, Marchesi JR. Transposon-aided capture (TRACA) of plasmids resident in the human gut mobile metagenome. Nat Methods 2007; 4:55-61.
24. Jones BV, Marchesi JR. Accessing the mobile metagenome of the human gut microbiota. Mol Biosyst 2007; 3:749-58.
25. Nikolich MP, Hong G, Shoemaker NB, Salyers AA. Evidence for natural horizontal transfer of tetQ between bacteria that normally colonize humans and bacteria that normally colonize livestock. Appl Environ Microbiol 1994; 60:3255-60.
26. Salyers AA, Shoemaker NB, Stevens AM, Li LY. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol Rev 1995; 59:579-90.
27. Kazimierczal KA, Scott KP, Kelly D, Aminov RI. Tetracycline resistome of the organic pig gut. Appl Environ Microbiol 2009; 75:171-1722.
28. Shoemaker NB, Vlamakis H, Hayes K, Salyers AA. Evidence for extensive resistance gene transfer among Bacteroides spp. and among Bacteroides and other genera in the human colon. Appl Environ Microbiol 2001; 67:561-8.
29. Gupta A, Vlamakis H, Shoemaker N, Salyers AA. A new Bacteroides conjugative transposon that carries an ermB gene. Appl Environ Microbiol 2003; 69:6455-63.
30. Wang YP, Wang GR, Shoemaker NB, Whitehead TR, Salyers AA. Distribution of the ermG gene among bacterial isolates from porcine intestinal contents. Appl Environ Microbiol 2005; 71:4930-4.
31. Salyers AA, Gupta A, Wang YP. Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends Microbiol 2004; 12:412-6.
32. Kazimierczak KA, Flint HJ, Scott KP. Comparative analysis of sequences flanking tet(W) resistance genes in multiple species of gut bacteria. Antimicrob Agents Chemother 2006; 50:2632-9.
33. Melville CM, Brunel R, Flint HJ, Scott KP. The Butyrivibrio fibrisolvens tet(W) gene is carried on the novel conjugative transposon TnB1230, which contains duplicated nitroreductase coding sequences. J Bacteriol 2004; 186:3656-9.
34. Melville CM, Scott KP, Mercer DK, Flint HJ. Novel tetracycline resistance gene, tet(32), in the Clostridiumrelated human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens. Antimicrob Agents Chemother 2001; 45:3246-9.
35. Scott KP, Melville CM, Barbosa TM, Flint HJ. Occurrence of the new tetracycline resistance gene tet(W) in bacteria from the human gut. Antimicrob Agents Chemother 2000; 44:775-7.
36. Shears P, Suliman G, Hart CA. Occurrence of multiple antibiotic resistant and R plasmids in Enterobacteriaceae isolated from children in the Sudan. Epidem Inf 1988; 100:73-81.
37. Trobos M, Lester CH, Olsen JE, Frimondt-MØller N, Hmmerum AM. Natural transfer of sulphonamide and amplicllin resistance between Escherichia coli residing in the human intestine. J Antimicrob Chemother 2009; 63:80-6.
38. Sommer MOA, Dantas G, Church GM. Functional characterisation of the antibiotic resistance reservoir in the human microflora. Science 2009; 325:1128-31.
39. Goran MG, Carmeli Y, Schwaber MJ, Chmelnitsky I, Schechner V, Navon-Venezia S. Transfer of carbapenem- resistant plasmid from Klebsiella pneumonias ST258 to Escherichia coli in patient. Emerg Infect Dis 2010; 16:1014-7.
40. Licht TN, Wilcks A. Conjugative gene transfer in the gastrointestinal environment. Adv Appl Microbiol 2006; 58:77-95.
41. Li Y, Canchaya C, Fang F, Raftis E, Ryan KA, van Pijkeren JP, et al. Distribution of megaplasmids in Lactobacillus salivarius and other Lactobacilli. Appl Environ Microbiol 2007; 189:6128-39.
42. Oshima K, Toh H, Ogura Y, Sasamoto H, Morita H, Park SH, et al. Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult. DNA Res 2008; 15:375-86.
43. Millette M, Dupont C, Sharek F, Ruiz MT, Archambault D, Lacroix M. Purification and identification of the pediocine produced by Pediococcus acidilactici MM33, a new human intestinal strain. J Appl Microbiol 2007; 104:269-75.
44. Claesson MJ, Li Y, Leahy S, Canchaya C, van Pijkeren JP, Cerdeño-Tárraga C, et al. Multireplicon genome architecture of Lactobacillus salivarius. Proc Natl Acad Sci USA 2006; 103:6718-28.
45. Moon K, Sonnenburg J, Salyers A. Unexpected effect of a Bacteriodes conjugative transposons, CtnDOT, on chromosomal gene expression in its bacterial host. Mol Microbiol 2007; 64:1562-72.
46. Chassey B, Gibson EM, Giuffrida A. Evidence for plasmid associated lactose metabolism in Lactobacillus casei ssp. casei. Curr Microbiol 1978; 1:141-4.
47. Muriana PM, Klaenhammer RT. Conjugal transfer of plasmid-encoded determinants for bacteriocin production and immunity in Lactobacillus acidophilus 88. Appl Environ Microbiol 1987; 53:553-60.
48. Tannock GW, Munro K, Harmsen HJ, Welling GW, Smart J, Gopal PK. Analysis of the faecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Appl Environ Microbiol 2000; 66:2578-88.
49. Zaneveld JR, Lozupone C, Gordon JI, Knight R. Ribosomal RNA diversity predicts genome diversity in gut bacteria and their relatives. Nucleic Acids Res 2010; 38:3869-79.
50. Blum-Oehler G, Oswald S, Eitejörge K, Sonnenborn U, Schulze J, Kruis W, et al. Development of strainspecific PCR reactions for the detection of the probiotic Escherichia coli strain Nissle 1917 in faecal samples. Res Microbiol 2003; 154:59-66.
51. Shankar N, Baghdayan AS, Gilmore MS. Modulation of virulence within an pathogenicity island in a vancomycin resistant Enterococcus faecalis. Nature 2002; 417:746-50.
52. Tzipori S, Gibson R, Montanaro J. Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors. Infect Immun 1989; 57:1142-50.
53. Dudley EG, Abe C, Ghigo JM, Latour-Lambert P, Hormazabal JC, Nataro JP. An IncI1 plasmid contributes to the adherence of the atypical enteroaggregative Escherichia coli strain C1096 to cultured cells and abiotic surfaces. Infect Immun 2006; 74:2102-14.
54. Rotger R, Casadesús J. The virulence plasmids of Samonella. Internatl Microbiol 1999; 2:177-84.
55. Boyd EF, Hartl D. Salmonella virulence plasmid: Modular acquisition of the spv virulence region by an F-plasmid in Salmonella enterica subspecies I and insertion into the chromosome of subspecies II, IIa, IV and Vii isolates. Genetics 1998; 149:1183-90.
56. Venkatesan MM, Goldberg MB, Rose DJ, Grotebeck EJ, Burland V, Blattner FR. Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri. Infect Immun 2001; 96:3271-85.
57. Xiong Z, Tang X, Yang F, Zhang X, Yang J, Chen L, et al. Comparison of the virulence plasmid genomes of two strains of Shigella which lost the ability to bind Congo red. Sci China C Life Sci 2006; 49:141-8.
58. Zagaglia C, Casalino M, Colonna B, Conti C, Calconi A, Nicoletti M. Virulence plasmids of enteroinvasive Escherichia coli and Shigella flexneri integrate into a specific site on the host chromosome: Integration greatly reduces expression of a plasmid-carried virulence genes. Infect Immun 1991; 59:729-99.
59. McCormick B, Siber AM, Maurelli AT. Requirement of the Shigella flexneri virulence plasmid in the ability to induce trafficking of neutrophils across polarized monolayers of the intestinal epithelium. Infect Immun 1998; 66:4237-43.
60. Gigho JM. Natural conjugative plasmids induce bacterial biofilm development. Nature 2001 412: 4442-5.
61. Flint HJ, Duncan SH, Scott KP, Louis P. Interactions and competition within the microbial community of the human colon: links between diet and health. Environ Microbiol 2007; 9:1101-11.
62. Huycke MM, Gaskins HR. Commensal bacteria, redox stress and colorectal cancer: mechanisms and models. Exp Biol Med 2004; 229:586-97.
63. Claesson MJ, Cusak S, O'Sullivan O, Greene-Dinaz R, de Weerd H, Flannery E, et al. Composition, variability and temporal stability of the intestinal microbiota of the elderly. Proc Natl Acad Sci USA 2010; DOI:10.1073/pnas.1000097107.
64. Biagi E, Nylund L, Candela M, Ostan R, Bucci L, Pini E, et al. Through ageing, and beyond: Gut microbiota and inflammatory status in seniors and centinarians. Plos ONE 2010; 5:10667.
65. Pang AW, MacDonald JR, Pinto D, Wei J, Rafiq MA, Conrad DF, et al. Towards a comprehensive structural variation map of an individual human genome. Genome Biol 2010; 11:52.
66. The Chimpanzee Sequencing and Analysis Consortium. Initial sequence of the chimpanzee genome and comparison with the human genome. Nature 2005; 437:69-87.
67. Denarie J, Boistard P, Cassedelbart F, Atherly AG, Berry JO, Russell P. Indigenous plasmids of Rhizobium. Int Rev Cytol 1981; 13:225-46.
68. Martinez E, Romero D, Palacios R. The Rhizobium genome. Crit Rev Plant Sci 1990; 9:59-93.
69. Top EM, Moenne-Loccoz Y, Pembroke T, Thomas CM. Phenotypic traits conferred by plasmids. In: Thomas CM, Ed. The Horizontal Gene Pool, Bacterial Plasmids and Gene Spread, Amsterdam: Harwood Academic Publishers 2000; 249-85.
70. Oliver KM, Degnan PH, Hunter MS, Moran NA. Bacteriophages encode factors required for protection in a symbiotic mutualism. Science 2009; 325:992-4.
71. Hehemann JK, Correc G, Barbeyon T, Helbert W, Czjzek M, Michel G. Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota. Nature 2010; 464:908-12.
72. Luzopone CA, Hamady M, Cantral BL, Coutinho PM, Henrissat B, Gordon JI, et al. The convergence of carbohydrate active gene repertoires in human gut microbes. Proc Natl Acadn Sci USA 2008; 105:15076-81.
73. Xu J, Mahowals MA, Ley RE, Lozupone CA, Hamady M, Martens EC, et al. Evolution of symbiotic bacteria in the distal human intestine. PLOS Biol 2007; 5:156.
74. Louis P, McCrae SI, Charrier C, Flint HJ. Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer. FEMS Microbiol Lett 2007; 269:240-7.
75. Netherwood T, Bowden R, Harrison P, O'Donnell AG, Parker DS, Gilbert HJ. Gene transfer in the gastrointestinal tract. Appl Environ Microbiol 1999; 65:5139-41.
76. Bardin S, Dan S, Osteras SM, Finan TM. A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti. J Bacteriol 1996; 178:4540-7.
77. Chen HC, Gartner E, Rolfe BG. Involvement of genes on a megaplasmid on the acid-tolerant phenotype of Rhizobium leguminsarum. Appl Environ Microbiol 1993; 59:1058-64.
78. Baldani JI, Weaver RW. Survival of clover rhizobia under heat and drought stresses in soil after curing plasmids. Soil Biol Biochem 1992; 24:737-42.
79. Barbour WM, Elkan GH. Relationship of the presence and copy number of plasmids to exopolysaccharide production and symbiotic effectiveness in a Rhizobium fredii ASDA 206. Appl Environ Microbiol 1989; 55:813-8.
80. McNiel NI. The contribution of the large intestine to energy supplies in man. Am J Clin Nutr 1984; 39:338-42.
81. Pryde SE, Duncan SH, Hold GL, Stewart CS, Flint HJ. The microbiology of butyrate formation in the human colon. FEMS Microbiol Lett 2002; 217:133-9.
82. Cummings JH, Antoine JM, Azpiroz, Bourdet-Sicard R, Brandtzaeg P, Calder PC, et al. PASSCLAIM1-Gut health and immunity. Eur J Nut 2004; 43:118-73.
83. McIntyre A, Gibson PR, Young GP. Butyrate production from dietary fiber and protection against large bowel cancer in a rat model. Gut 1993; 34:86-391.
84. Sponheimer M, Passey BH, de Ruiter DJ, Guatelli- Steinberg D, Cerling TE, Lee-Thorp JA. Isotopic evidence for dietary variability in the early hominin Paranthropus robustus. Science 2006; 314:980-9822.
85. Sponheimer M, Lee-Thorp JA. Isotopic evidence for dietary variability in the early hominid Australopithecus africanus. Science 1999; 283:368-70.
86. Igimi S, Ryu CH, Park SH, Sasaki Y, Sasaki T, Kumagai S. Transfer of conjugative plasmid pAMbeta1 from Lactococcus lactis to mouse intestinal bacteria. Lett Appl Microbiol 1996; 23:31-5.
87. Tuohy K, Davies M, Rumsby P, Rumney C, Adams MR, Rowland IR. Monitoring transfer of recombinant and non-recombinant plasmids between Lactococcus lactis strains and members of the human gastrointestinal microbiota in vivo -impact of donor cell number and diet. J Appl Microbiol 2002; 93:954-64.
88. Fang F, Li Y, Bumann M, Raftis EJ, Casey PG, Cooney JC, et al. Allelic variation of bile salt hydrolase genes in Lactobacillus salivarius does not determine bile resistance levels. J Bacteriol 2009; 191:5743-57.
89. Begley M, Hill C, Gahan CG. Bile salt hydrolase activity in probiotics. Appl Environ Microbiol 2006; 72:1729-38.
90. Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA, et al. Environmental genome shotgun sequencing of the Sargasso Sea. Science 2004; 304:66-74.
91. Tringe SG, von Mering C, Kobayashi A, Salamov AA, Chen K, Chang HW, et al. Comparative metagenomics of microbial communities. Science 2005; 308:554-7.
92. Breitbart M, Hewson I, Felts B, Mahaffy JM, Nulton J, Salamon P, et al. Metagenomic analyses of an uncultured viral community from human feces. J Bacteriol 2003; 185:6220-3.
93. Ebdon J, Muniesa M, Taylor H. The application of a recently isolated strain of Bacteroides (GB-124) to identify human sources of faecal pollution in a temperate river catchment. Water Res 2007; 41:3683-90.
94. Górski A, Wazna E, Dabrowska BW, Dabrowska K, Switala-Jelen K, Miedzybrodzki R. Bacteriophage translocation. FEMS Immunol Med Microbiol 2006; 46:313-9.
95. Pandey DP, Gerdes K. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res 2005; 33:966-76.
96. Van Melderen L, De Bast MS. Bacterial toxin-antitoxinsystems: More than selfish entities? Plos Genet 2009; 5:1000437.
97. Overgaard M, Borch J, Jørgensen MG, Gerdes K. Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity. Mol Microbiol 2008; 69:841-57.
98. Gerdes K, Møller-Jensen J, Bugge Jensen R. Plasmid and chromosome partitioning: surprises from phylogeny. Mol Microbiol 2000; 37:455-66.
99. Tsilibaris V, Maenhaut-Michel G, Mine N, Van Melderen L. What Is the Bene?t to Escherichia coli of Having Multiple Toxin-Antitoxin Systems in Its Genome? J Bacteriol 2007; 189:6101-8.
100. Christensen SK, Pedersen K, Hansen FG, Gerdes K. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA. J Mol Biol 2003; 332:809-19.
101. Christensen SK, Mikkelsen M, Pedersen K, Gerdes K. ReIE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci USA 2001; 98:14328-33.
102. Anantharaman V, Aravind L. New connections in the prokaryotic toxin-antitoxin network: Relationship with the eukaryotic nonsense-mediated RNA decay System. Genome Biol 2003; 4:81.
103. Vázquez-Laslop N, Lee H, Neyfakh AA. Increased persistence in Escherichia coli caused by controlled expression of toxins or other unrelated proteins. J Bacteriol 2006; 188:3494-7.
104. Nieto C, Sadowy E, de la Campa AG, Hryniewicz W, Espinosa M. The relBE2Spn toxin-antitoxin system of Streptocococcus pneumonia: Role in antibiotic tolerance and functional conservation in clinical isolates. Plos One 2010; 5:11289.
105. Yamamoto TA, Gerdes K, Tunnacliffe A. Bacterial toxin RelE induces apoptosis in human cells. FEBS Lett 2002; 519:191-4.
106. Kristoffersen P, Jensen GB, Gerdes K, Piskur J. Bacterial toxin-antitoxin gene system as containment control in yeast cells. Appl Environ Microbiol 2000; 66:5524-6.
107. Andreev D, Hauryliuk V, Terenin I, Dmitriev S, Ehrenberg M, Shatsky I. The bacterial toxin ReIE induces specific mRNA cleavage in the A site of the eukaryote ribosome. RNA 2008; 14:233-9.
108. Rohwer F, Prangishvili D, Lindell D. Roles of viruses in the environment. Environ Microbiol 2009; 11:2771-4.
109. Lepage P, Colombet J, Marteau P, Sime-Ngando T, Doré J, Leclerc M. Dysbiosis in inflammatory bowel disease: a role for bacteriophages? Gut 2008; 57:424-5.
110. Saitoh S, Noda S, Aiba Y, Takagi A, Sakamoto M, Benno Y, et al. Bacteroides ovatus as the predominant commensal intestinal microbe causing a systemic antibody response in inflammatory bowel disease. Clin Diagnost Lab Immunol 2002; 9:54-9.
111. Lenth RV. (2009) Java applets for power and sample size [Piface.jar Version 1.70, March 2009], http://www.stat.uiowa.edu/~rlenth/Power.
112. Saavedra De Bast M, Mine N, Van Melderen L. Chromosomal toxin-antitoxin systems may act as antiaddiction modules. J Bacteriol 2008; 190:4603-9.
113. Pecota CD, Wood TK. Exclusion of T4 phage by the hok/sok killer locus from plasmid R1. J Bacteriol 1996; 178:2044-50.
114. Doyle M, Fookes M, Ivens A, Mangan MW, Wain J, Dorman CJ. A H-NS-like stealth protein aids horizontal gene transmission in bacteria. Science 2007; 315:251-2.
115. Kim Y, Wang X, Ma Q, Zhang XS, Wood TK. Toxin-antitoxin systems in Escherichia coli influence biofilm formation through YigK (TabA) and fimbriae. J Bacteriol 2009; 191:1258-67.
116. Bollinger RR, Barbas AS, Bush EL, Lin SS, Parker W. Biofilms in the normal human large bowel: Fact rather than fiction. Gut 2007; 56:1481-2.
117. Kleessen B, Blaut M. Modulation of gut mucosal biofilms. Br J Nutr 2005; 93:35.