Development of strain-specific PCR reactions for the detection of the probiotic Escherichia coli strain Nissle 1917 in fecal samples

Research in Microbiology

Volumn 154, Issue 1, 2003, Pages 59-66

  Blum Oehler, Gabriele ^a   Oswald, Sibylle ^a   Eiteljörge, Karin ^b   Sonnenborn, Ulrich ^b   Schulze, Jürgen ^b   Kruis, Wolfgang ^c   Hacker, Jörg ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b Ardeypharm GmbH   (Germany)

^c Evangelisches Krankenhaus Kalk   (Germany)

Author keywords

Escherichia coli; Feces; PCR; Plasmids; Probiotics

Indexed keywords

MUTAFLOR; PRIMER DNA; PROBIOTIC AGENT; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENTEROPATHY; ENVIRONMENT; ESCHERICHIA COLI; FECES; FECES ANALYSIS; FECES MICROFLORA; FIMBRIA; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRAIN DIFFERENCE; ULCERATIVE COLITIS; VALIDATION PROCESS;

DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; FECES; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ESCHERICHIA COLI;

EID: 0037255550     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(02)00007-4     Document Type: Article

References (31)

1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R.P. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect. Immun. 39:1983;315-335.
2. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
3. Avison M.B., Walsh T.R., Bennett P.M. pUB6060: A broad-host-range, DNA polymerase-I-independent ColE2-like plasmid. Plasmid. 45:2001;88-100.
4. Bachmann B.J. Derivatives and genotypes of some mutant derivatives of Escherichia coli K-12. Neidhardt F.C., Ingraham J.L., Low K.B., Magasanik B., Schaechter M., Umbarger H.E. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. 1st Edition:1987;1190-1219 ASM Press, Washington, DC.
5. Blum G., Marre R., Hacker J. Properties of Escherichia coli strains of serotype O6. Infection. 23:1995;234-236.
6. Boom R., Sol C.J., Salimans M.M.M., Jansen C.L., Wertheim-van Dillen P.M.E., Noordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:1990;495-503.
7. Boyer H.W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:1969;459-472.
8. Burian J., Guller L., Macor M., Kay W.W. Small cryptic plasmids of multiplasmid, clinical Escherichia coli. Plasmid. 37:1997;2-14.
9. Deng W., Li Y., Vallance B.A., Finlay B.B. Locus of enterocyte effacement from Citrobacter rodentium: Sequence analysis and evidence for horizontal transfer among attaching and effacing pathogens. Infect. Immun. 69:2001;6323-6335.
10. Feldgarden M., Golden S., Wilson H., Riley M.A. Can phage defence maintain colicin plasmids in Escherichia coli? Microbiology. 141:1995;2977-2984.
11. Hacker J., Dobrindt U., Emödy L. Wie Bakterien kommunizieren: Quorum sensing und Crosstalk in bakteriellen Lebensgemeinschaften. Alfred Nissle Gesellschaft e.V. (Ed.), Darmflora in Symbiose und Pathogenität. 2001;73-81 Hagen, Germany.
12. ®), Internal Company Report. 1998;Pharma-Zentrale GmbH, Herdecke, Germany.
13. Johnson J.R. Urinary tract infection. Sussman M. Escherichia coli: Mechanisms of Virulence. 1997;495-549 Cambridge University Press, Cambridge.
14. Kruis W., Schütz E., Fric P., Fixa B., Judmaier G., Stolte M. Double-blind comparison of an oral Escherichia coli preparation and mesalazine in maintaining remission of ulcerative colitis. Aliment. Pharmacol. Ther. 11:1997;853-858.
15. Kruis W., Fric P., Stolte M. The Mutaflor study group. Maintenance of remission in ulcerative colitis is equally effective with Escherichia coli Nissle 1917 and with standard mesalamine. Gastroenterology. 120:(Suppl.):2001;A127.
16. Kuhnert P., Nicolet J., Frey J. Rapid and accurate identification of Escherichia coli K-12 strains. Appl. Environ. Microbiol. 61:1995;4135-4139.
17. Lane D.J. 16S/23S rRNA sequencing. Stackebrandt E., Goodfellow M. Nucleic Acid Techniques in Bacterial Systematics. 1991;131-148 Wiley, Chichester.
18. Lodinová-Zadníková R., Sonnenborn U. Effect of preventive administration of a nonpathogenic Escherichia coli strain on the colonization of the intestine with microbial pathogens in newborn infants. Biol. Neonate. 71:1997;224-232.
19. Malchow H., Sonnenborn U., Greinwald R., Körner A. Colonization of adults by a non-pathogenic Escherichia coli strain. Gastroenterology. 108:(Suppl.):1995;A869.
20. Malchow H. Crohn's disease and E. coli: A new approach in therapy fo maintain remission of colonic Crohn's disease? J. Clin. Gastroenterol. 25:1997;653-658.
21. Mason T.G., Richardson G. Escherichia coli and the human gut: Some ecological considerations. J. Appl. Bacteriol. 51:1981;1-16.
22. Möllenbrink M., Bruckschen E. Mikrobiologische Behandlung der chronischen Obstipation. Med. Klin. 89:1994;587-593.
23. Mühldorfer I., Hacker J. Genetic aspects of Escherichia coli virulence. Microb. Pathog. 16:1994;171-181.
24. Mühldorfer I., Blum G., Donohue-Rolfe A., Heier H., Ölschläger T., Tschäpe H., Wallner U., Hacker J. Characterization of Escherichia coli strains isolated from environmental water habitats and from stool samples of healthy volunteers. Res. Microbiol. 147:1996;625-635.
25. Nissle A. Die antagonistische Behandlung chronischer Darmstörungen mit Colibakterien. Med. Klin. 2:1918;29-30.
26. Nissle A. Weiteres über Grundlagen und Praxis der Mutaflorbehandlung. DMW. 4:1925;1809-1813.
27. Rembacken B.J., Snelling A.M., Hawkey P.M., Chalmers D.M., Axon A.T.R. Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis. A randomised trial. Lancet. 42:1999;635-639.
28. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd Edition:1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Sanger F., Niklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
30. Van Die I., Kramer C., Hacker J., Bergmans H., Jongen W., Hoekstra W. Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli. Res. Microbiol. 142:1991;653-658.
31. Zingler G., Blum G., Falkenhagen U., Ørskov I., Ørskov F., Hacker J., Ott M. Clonal differentiation of uropathogenic Escherichia coli isolates of serotype O6:K5 by fimbrial antigen typing and DNA long-range mapping techniques. Med. Microbiol. Immunol. 182:1993;13-24.