Mitochondrial mass is inversely correlated to complete lipid oxidation in human myotubes

Biochemical and Biophysical Research Communications

Volumn 404, Issue 4, 2011, Pages 1023-1028

  Gaster, Michael ^a  

^a ODENSE UNIVERSITY HOSPITAL   (Denmark)

Author keywords

ATP; Human skeletal muscle; Mitochondria; Mitochondrial mass; Myotubes; Substrate oxidation; Type 2 diabetes

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; GLUCOSE;

ADULT; ARTICLE; CELL STRUCTURE; CLINICAL ARTICLE; CONTROLLED STUDY; GLUCOSE OXIDATION; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VIVO STUDY; LEAN BODY WEIGHT; LIPID OXIDATION; MITOCHONDRION; MYOTUBE; NON INSULIN DEPENDENT DIABETES MELLITUS; OBESITY; PRIORITY JOURNAL; SKELETAL MUSCLE;

DIABETES MELLITUS, TYPE 2; EXERCISE; HUMANS; LIPID METABOLISM; MIDDLE AGED; MITOCHONDRIA, MUSCLE; MUSCLE FIBERS, SKELETAL; OBESITY; OXIDATION-REDUCTION;

EID: 79151485987     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.12.102     Document Type: Article

References (32)

1. Lanza I.R., Nair K.S. Muscle mitochondrial changes with aging and exercise. Am. J. Clin. Nutr. 2009, 89:467S-471S.
2. Hebert S.L., Lanza I.R., Nair K.S. Mitochondrial DNA alterations and reduced mitochondrial function in aging. Mech. Ageing Dev. 2010, 131:451-462.
3. Kelley D.E., He J., Menshikova E.V., Ritov V.B. Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes. Diabetes 2002, 51:2944-2950.
4. Schrauwen-Hinderling V.B., Kooi M.E., Hesselink M.K., Jeneson J.A., Backes W.H., van Echteld C.J., van Engelshoven J.M., Mensink M., Schrauwen P. Impaired in vivo mitochondrial function but similar intramyocellular lipid content in patients with type 2 diabetes mellitus and BMI-matched control subjects. Diabetologia 2007, 50:113-120.
5. Kelley D.E., Simoneau J.A. Impaired free fatty acid utilization by skeletal muscle in non-insulin-dependent diabetes mellitus. J. Clin. Invest. 1994, 94:2349-2356.
6. Lanza I.R., Nair S. Regulation of skeletal muscle mitochondrial function:genes to proteins. Acta Physiol. 2010, 199:529-547.
7. Minet A.D., Gaster M. ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects. Biochem. Biophys. Res. Commun. 2010, 402:70-74.
8. Frederiksen C.M., Hojlund K., Hansen L., Oakeley E.J., Hemmings B., Abdallah B.M., Brusgaard K., Beck-Nielsen H., Gaster M. Transcriptional profiling of myotubes from patients with type 2 diabetes: no evidence for a primary defect in oxidative phosphorylation genes. Diabetologia 2008, 51:2068-2077.
9. Gaster M., Rustan A.C., Aas V., Beck-Nielsen H. Reduced lipid oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic orig evidence from cultured myotubes. Diabetes 2004, 53:542-548.
10. Gaster M., Rustan A., Beck-Nielsen H. Differential utilization of saturated palmitate unsaturated oleate. Evidence from cultured myotubes. Diabetes 2005, 54:648-656.
11. Wensaas A.J., Rustan A.C., Just M., Berge R.K., Drevon C.A., Gaster M. Fatty acid incubation of myotubes from humans with type 2 diabetes leads to enhanced release of beta-oxidation products because of impaired fatty acid oxidation: effects of tetradecylthioacetic acid and eicosapentaenoic acid. Diabetes 2009, 58:527-535.
12. Gaster M., Kristensen S.R., Beck-Nielsen H., Schroder H.D. A cellular model system of differentiated human myotubes. APMIS 2001, 109:735-744.
13. Ortenblad N., Mogensen M., Petersen I., Hojlund K., Levin K., Sahlin K., Beck-Nielsen H., Gaster M. Reduced insulin-mediated citrate synthase activity in cultured skeletal muscle cells from patients with type 2 diabetes: evidence for an intrinsic oxidative enzyme defect. Biochim. Biophys. Acta 2005, 1741:206-214.
14. Gaster M., Beck-Nielsen H. Triacylglycerol accumulation is not primarily affected in myotubes established from type 2 diabetic subjects. Biochim. Biophys. Acta 2006, 1761:100-110.
15. Gaster M., Beck-Nielsen H., Schroder H.D. Proliferation conditions for human satellite cells. The fractional content of satellite cells. APMIS 2001, 109:726-734.
16. Gaster M., Schroder H.D., Handberg A., Beck-Nielsen H. The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure. Biochim. Biophys. Acta 2001, 1537:211-221.
17. Skrede S., Bremer J., Berge R.K., Rustan A.C. Stimulation of fatty acid oxidation by a 3-thia fatty acid reduces triacylglycerol secretion in cultured rat hepatocytes. J. Lipid Res. 1994, 35:1395-1404.
18. Kristensen S.R. A critical appraisal of the association between energy charge and cell damage. Biochim. Biophys. Acta 1989, 1012:272-278.
19. Andersen P., Saltin B. Maximal perfusion of skeletal muscle in man. J. Physiol. 1985, 366:233-249.
20. Kelley D.E., Mandarino L.J. Fuel selection in human skeletal muscle in insulin resistance: a reexamination. Diabetes 2000, 49:677-683.
21. Gaster M. Reduced TCA flux in diabetic myotubes: a governing influence on the diabetic phenotype?. Biochem. Biophys. Res. Commun. 2009, 387:651-655.
22. Befroy D.E., Petersen K.F., Dufour S., Mason G.F., de Graaf R.A., Rothman D.L., Shulman G.I. Impaired mitochondrial substrate oxidation in muscle of insulin-resistant offspring of type 2 diabetic patients. Diabetes 2007, 56:1376-1381.
23. Schrauwen P., Hesselink M.K. Reduced tricarboxylic acid cycle flux in type 2 diabetes mellitus?. Diabetologia 2008, 51:1694-1697.
24. Randle P.J., Garland P.B., Hales C.N., Newsholme E.A. The glucose fatty-acid cycle. Its role in insulin sensitivity the metabolic disturbances of diabetes mellitus. Lancet 1963, 1:785-789.
25. Sidossis L.S., Stuart C.A., Shulman G.I., Lopaschuk G.D., Wolfe R.R. Glucose plus insulin regulate fat oxidation by controlling the rate of fatty acid entry into the mitochondria. J. Clin. Invest. 1996, 98:2244-2250.
26. Turcotte L.P., Swenberger J.R., Yee A.J. High carbohydrate availability increases LCFA uptake and decreases LCFA oxidation in perfused muscle. Am. J. Physiol Endocrinol. Metab. 2002, 282:p. E177-E183.
27. Sidossis L.S., Wolfe R.R. Glucose and insulin-induced inhibition of fatty acid oxidation: the glucose-fatty acid cycle reversed. Am. J. Physiol. 1996, 270:E733-E738.
28. Gaster M. Substrate overload: glucose oxidation in human myotubes conquers palmitate oxidation through anaplerosis. Biochem. Biophys. Res. Commun. 2010, 391:1369-1373.
29. Gaster M. Insulin resistance the mitochondrial link. Lessons from cultured human myotubes. Biochim. Biophys. Acta 2007, 1772:755-765.
30. Gaster M. Reduced lipid oxidation in myotubes established from obese and type 2 diabetic subjects. Biochem. Biophys. Res. Commun. 2009, 382:766-770.
31. Janssen A.J., Trijbels F.J., Sengers R.C., Wintjes L.T., Ruitenbeek W., Smeitink J.A., Morava E., van Engelen B.G., van den Heuvel L.P., Rodenburg R.J. Measurement of the energy-generating capacity of human muscle mitochondria: diagnostic procedure and application to human pathology. Clin. Chem. 2006, 52:860-871.
32. McBride A., Hardie D.G. AMP-activated protein kinase--a sensor of glycogen as well as AMP and ATP?. Acta Physiol. (Oxf) 2009, 196:99-113.