Insulin resistance and the mitochondrial link. Lessons from cultured human myotubes

Biochimica et Biophysica Acta - Molecular Basis of Disease

Volumn 1772, Issue 7, 2007, Pages 755-765

  Gaster, Michael ^a  

^a ODENSE UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Glucose metabolism; Insulin resistance; Lipid metabolism; Metabolic inhibition; Mitochondrial function; Myotube; Skeletal muscle; Type 2 diabetes

Indexed keywords

2,4 DINITROPHENOL; ANTIMYCIN A1; GLUCOSE; GLYCOGEN; LACTIC ACID; OLIGOMYCIN; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE;

ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; GLUCOSE METABOLISM; GLUCOSE OXIDATION; GLUCOSE TRANSPORT; GLYCOGEN SYNTHESIS; HUMAN; HUMAN CELL; INSULIN RESISTANCE; LIPID METABOLISM; LIPID OXIDATION; LIPID TRANSPORT; METABOLIC INHIBITION; MITOCHONDRION; MYOTUBE; NON INSULIN DEPENDENT DIABETES MELLITUS; PATHOPHYSIOLOGY; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL; RESPIRATORY CHAIN; SKELETAL MUSCLE;

2,4-DINITROPHENOL; ANTIMYCIN A; CASE-CONTROL STUDIES; CELLS, CULTURED; DIABETES MELLITUS, TYPE 2; GLUCOSE; HUMANS; INSULIN RESISTANCE; LACTIC ACID; MIDDLE AGED; MITOCHONDRIA, MUSCLE; OBESITY; OLIGOMYCINS; OXIDATION-REDUCTION;

EID: 34250021550     PISSN: 09254439     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbadis.2007.03.007     Document Type: Article

References (28)

1. Simoneau J.A., and Kelley D.E. Altered glycolytic and oxidative capacities of skeletal muscle contribute to insulin resistance in NIDDM. J. Appl. Physiol. 83 (1997) 166-171
2. Kelley D.E., He J., Menshikova E.V., and Ritov V.B. Dysfunction of mitochondria in human skeletal muscle in type 2 diabetes. Diabetes 51 (2002) 2944-2950
3. Stump C.S., Short K.R., Bigelow M.L., Schimke J.M., and Nair K.S. Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 7996-8001
4. Mootha V.K., Lindgren C.M., Eriksson K.F., Subramanian A., Sihag S., Lehar J., Puigserver P., Carlsson E., Ridderstrale M., Laurila E., Houstis N., Daly M.J., Patterson N., Mesirov J.P., Golub T.R., Tamayo P., Spiegelman B., Lander E.S., Hirschhorn J.N., Altshuler D., and Groop L.C. PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat. Genet. 34 (2003) 267-273
5. Patti M.E., Butte A.J., Crunkhorn S., Cusi K., Berria R., Kashyap S., Miyazaki Y., Kohane I., Costello M., Saccone R., Landaker E.J., Goldfine A.B., Mun E., DeFronzo R., Finlayson J., Kahn C.R., and Mandarino L.J. Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 8466-8471
6. Sreekumar R., Halvatsiotis P., Schimke J.C., and Nair K.S. Gene expression profile in skeletal muscle of type 2 diabetes and the effect of insulin treatment. Diabetes 51 (2002) 1913-1920
7. Petersen K.F., Dufour S., Befroy D., Garcia R., and Shulman G.I. Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes. N. Engl. J. Med. 350 (2004) 664-671
8. Gaster M., Rustan A.C., Aas V., and Beck-Nielsen H. Reduced lipid oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin: evidence from cultured myotubes. Diabetes 53 (2004) 542-548
9. Gaster M., and Beck-Nielsen H. The reduced insulin mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin-Evidence from cultured myotubes. Biochim. Biophys. Acta 1690 (2004) 85-91
10. Ortenblad N., Mogensen M., Petersen I., Hojlund K., Levin K., Sahlin K., Beck-Nielsen H., and Gaster M. Reduced insulin-mediated citrate synthase activity in cultured skeletal muscle cells from patients with type 2 diabetes: evidence for an intrinsic oxidative enzyme defect. Biochim. Biophys. Acta 1741 (2005) 206-214
11. Gaster M., Petersen I., Hojlund K., Poulsen P., and Beck-Nielsen H. The diabetic phenotype is conserved in myotubes established from diabetic subjects: evidence for primary defects in glucose transport and glycogen synthase activity. Diabetes 51 (2002) 921-927
12. Henry R.R., Ciaraldi T.P., Abrams-Carter L., Mudaliar S., Park K.S., and Nikoulina S.E. Glycogen synthase activity is reduced in cultured skeletal muscle cells of non-insulin-dependent diabetes mellitus subjects. Biochemical and molecular mechanisms. J. Clin. Invest. 98 (1996) 1231-1236
13. Henry R.R., Ciaraldi T.P., Mudaliar S., Abrams L., and Nikoulina S.E. Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells: influence of high glucose and insulin levels. Diabetes 45 (1996) 400-407
14. Gaster M., Kristensen S.R., Beck-Nielsen H., and Schroder H.D. A cellular model system of differentiated human myotubes. APMIS 109 (2001) 735-744
15. Gaster M., Schroder H.D., Handberg A., and Beck-Nielsen H. The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure. Biochim. Biophys. Acta 1537 (2001) 211-221
16. Passonneau J.V., and Lowry O.H. Enzymatic Analysis. A Practical Guide (1993), Humana Press, Totowa, NJ
17. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
18. Skrede S., Bremer J., Berge R.K., and Rustan A.C. Stimulation of fatty acid oxidation by a 3-thia fatty acid reduces triacylglycerol secretion in cultured rat hepatocytes. J. Lipid Res. 35 (1994) 1395-1404
19. Rabol R., Boushel R., and Dela F. Mitochondrial oxidative function and type 2 diabetes. Appl. Physiol. Nutr. Metab. 31 (2006) 675-683
20. Ritov V.B., Menshikova E.V., He J., Ferrell R.E., Goodpaster B.H., and Kelley D.E. Deficiency of subsarcolemmal mitochondria in obesity and type 2 diabetes. Diabetes 54 (2005) 8-14
21. Pan D.A., Lillioja S., Kriketos A.D., Milner M.R., Baur L.A., Bogardus C., Jenkins A.B., and Storlien L.H. Skeletal muscle triglyceride levels are inversely related to insulin action. Diabetes 46 (1997) 983-988
22. Phillips D.I., Caddy S., Ilic V., Fielding B.A., Frayn K.N., Borthwick A.C., and Taylor R. Intramuscular triglyceride and muscle insulin sensitivity: evidence for a relationship in nondiabetic subjects. Metabolism 45 (1996) 947-950
23. Goodpaster B.H., Thaete F.L., and Kelley D.E. Thigh adipose tissue distribution is associated with insulin resistance in obesity and in type 2 diabetes mellitus. Am. J. Clin. Nutr. 71 (2000) 885-892
24. Krssak M., Falk P.K., Dresner A., DiPietro L., Vogel S.M., Rothman D.L., Roden M., and Shulman G.I. Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans: a 1H NMR spectroscopy study. Diabetologia 42 (1999) 113-116
25. He J., Watkins S., and Kelley D.E. Skeletal muscle lipid content and oxidative enzyme activity in relation to muscle fiber type in type 2 diabetes and obesity. Diabetes 50 (2001) 817-823
26. Simoneau J.A., Colberg S.R., Thaete F.L., and Kelley D.E. Skeletal muscle glycolytic and oxidative enzyme capacities are determinants of insulin sensitivity and muscle composition in obese women. FASEB J. 9 (1995) 273-278
27. Perseghin G., Scifo P., De Cobelli F., Pagliato E., Battezzati A., Arcelloni C., Vanzulli A., Testolin G., Pozza G., Del Maschio A., and Luzi L. Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans: a 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents. Diabetes 48 (1999) 1600-1606
28. Gaster M., and Beck-Nielsen H. Triacylglycerol accumulation is not primarily affected in myotubes established from type 2 diabetic subjects. Biochim. Biophys. Acta 1761 (2006) 100-110