Phosphotriesterase variants with high methylphosphonatase activity and strong negative trade-off against phosphotriesters

Protein Engineering, Design and Selection

Volumn 24, Issue 1-2, 2011, Pages 151-159

  Briseño Roa, Luis ^a,b   Timperley, Christopher M ^c   Griffiths, Andrew D ^d   Fersht, Alan R ^a  

^a MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c DEFENCE SCIENCE AND TECHNOLOGY LABORATORY   (United Kingdom)

^d UNIVERSITÉ LOUIS PASTEUR   (France)

Author keywords

nerve agents; phosphotriesterase; screening; trade off

Indexed keywords

BIOCHEMISTRY; BIOTECHNOLOGY; PROTEINS; SCREENING;

CATALYTIC EFFICIENCIES; DOCKING SIMULATIONS; HIGH-RESOLUTION STRUCTURES; NERVE AGENTS; ORDERS OF MAGNITUDE; PHOSPHOTRIESTERASES; PSEUDOMONAS DIMINUTA; TRADE OFF;

ECONOMIC AND SOCIAL EFFECTS;

CYCLOSARIN; ENZYME VARIANT; ESTER; ISOLEUCINE; LEUCINE; METHYLPHOSPHONATASE; METHYLPHOSPHONIC ACID; PESTICIDE; PHOSPHOTRIESTERASE; SARIN; SERINE; SOMAN; UNCLASSIFIED DRUG;

ARTICLE; ASYMMETRIC CATALYSIS; BREVUNDIMONAS DIMINUTA; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE MUTATION; HYDROLYSIS; HYDROPHOBICITY; LOSS OF FUNCTION MUTATION; MOLECULAR DOCKING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RACEMIC MIXTURE; SIMULATION;

BREVUNDIMONAS DIMINUTA;

EID: 78650465202     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq076     Document Type: Article

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