Mutagenesis of organophosphorus hydrolase to enhance hydrolysis of the nerve agent VX

Biochemical and Biophysical Research Communications

Volumn 279, Issue 2, 2000, Pages 516-519

  Gopal, Sriram ^a   Rastogi, Vipin ^b   Ashman, William ^b   Mulbry, Walter ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b U S ARMY   (United States)

Author keywords

Chemical warfare agent; Nerve agent; OPH; Organophosphorus hydrolase; Parathion; VX

Indexed keywords

ACETYLCHOLINESTERASE; CHEMICAL WARFARE AGENT; DYFLOS; HYDROLASE; PARAOXON; PARATHION; PESTICIDE;

ARTICLE; CRYSTAL STRUCTURE; HYDROLYSIS; MUTAGENESIS; PRIORITY JOURNAL;

EID: 0034694836     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.4004     Document Type: Article

References (23)

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2. Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by Southern hybridization with opd from Pseudomonas diminuta
3. Parathion hydrolase specified by the Flavobacterium opd gene: Relationship between gene and protein
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5. Microbial metabolism and enzymology of selected pesticides
6. Inactivation of organophosphous nerve agents by the phosphotriesterase from Pseudomonas diminuta
7. Characterization of P-S bond hydrolysis in organophosphorothioate by organophosphorus hydrolase
8. Enzymatic hydrolysis of the chemical warfare agent VX and its neurotoxic analogues by organophosphorus hydrolase
9. Three-dimensional structure of phosphotriesterase: An enzyme capable of detoxifying organophosphate nerve agents
10. Three-dimensional structure of the binuclear metal center of phosphotriesterase
11. Hydrolysis of Tetriso by an enzyme derived from Pseudomonas diminuta as a model for the detoxification of VX
12. Enzymatic hydrolysis of Russian-VX by organophosphorus hydrolase
13. Biodegradation of chemical warfare agents
14. Molecular modeling study of acetylcholinesterase from Torpedo californica and organophosphorus hydrolase from Pseudomonas diminuta and a protein engineering design for enhancement of VX hydrolysis activity
15. Molecular mechanic study of nerve agent S-[2-(diisopropylamino)ethyl] methyl-phosphonothioate (VX) bound to the active site of Torpedo californica Acetylcholinesterase
16. Augmented hydrolysis of diisopropyl fluorophosphate in engineered mutants of phosphotriesterase
17. Rational design of organophosphorus hydrolase for altered substrate specificities
18. Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate
19. A new and rapid calorimetric determination of acetylcholinesterase activity
20. Determination of enzyme dissociation constants
21. Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase
22. Three distinct domains in the cholinesterase molecule confer selectivity for acetyl- and butyrylcholinesterase inhibitors