Validation of DNA and RNA real-time assays for food analysis using the hilA gene of Salmonella enterica serovars

Journal of Microbiological Methods

Volumn 84, Issue 1, 2011, Pages 19-26

  McCabe, Evonne M ^a,b   Burgess, Catherine M ^a   Walsh, Des ^a   O'Regan, Edel ^b   McGuinness, Sheila ^c   Barry, Thomas ^c   Fanning, Séamus ^b   Duffy, Geraldine ^a  

^a ASHTOWN FOOD RESEARCH CENTRE   (Ireland)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

^c NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

DNA; HilA; ISO 16140:2003; ISO 6759:2004; Real time PCR; RNA; Salmonella enterica; Validation

Indexed keywords

BACTERIAL PROTEIN; DNA; PROTEIN HILA; RNA; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CARCASS; COLONY FORMING UNIT; CONTROLLED STUDY; DNA DETERMINATION; FOOD ANALYSIS; INOCULATION; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; SEROTYPE; VALIDATION PROCESS;

BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; EUROPE; FOOD ANALYSIS; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RNA, BACTERIAL; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; TRANS-ACTIVATORS;

SALMONELLA ENTERICA; SALMONELLA SP.;

EID: 78650204952     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.10.004     Document Type: Article

References (34)

1. Aabo S., Andersen J.K., Olsen J.E. Research note: detection of Salmonella in minced meat by the polymerase chain reaction method. Lett Appl Microbiol 1995, 21:180-182.
2. Churchill R.L.T., Lee H., Hall J.C. Emerging bacterial foodborne pathogens and methods of detection. Food Biochemistry and Food Processing 2007, 705-744. Y.H. Hui (Ed.).
3. EC 2073 Commision regulation (EC) no. 2073/2005 on microbiological criteria for foodstuffs. Off J Eur Union 2005, L338:1-25.
4. Ellingson J.L., Anderson J.L., Carlson S.A., Sharma V.K. Twelve hour real-time PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products. Mol Cell Probes 2004, 18:51-57.
5. Fakhr M.K., McEvoy J.M., Sherwood J.S., Logue C.M. Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products. Lett Appl Microbiol 2006, 43:78-83.
6. Fey A., Eichler S., Flavier S., Christen R., Hofle M.G., Guzman C.A. Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism. Appl Environ Microbiol 2004, 70:3618-3623.
7. Hein I., Flekna G., Krassnig M., Wagner M. Real-time PCR for the detection of Salmonella spp. in food: an alternative approach to a conventional PCR system suggested by the FOOD-PCR project. J Microbiol Methods 2006, 66:538-547.
8. Hendriksen R.S., Seyfarth A.M., Jensen A.B., Whichard J., Karlsmose S., Joyce K., Mikoleit M., Delong S.M., Weill F.X., Aidara-Kane A., Lo Fo Wong D.M., Angulo F.J., Wegener H.C., Aarestrup F.M. WHO Global Salm-Surv External Quality Assurance System (EQAS) for antimicrobial susceptibility testing of Salmonella isolates, 2000-2007. J Clin Microbiol 2009, 47:2729-2736.
9. Herrera-Leon S., Ramiro R., Arroyo M., Diez R., Usera M.A., Echeita M.A. Blind comparison of traditional serotyping with three multiplex PCRs for the identification of Salmonella serotypes. Res Microbiol 2007, 158:122-127.
10. Hoorfar J., Ahrens P., Radstrom P. Automated 5' nuclease PCR assay for identification of Salmonella enterica. J Clin Microbiol 2000, 38:3429-3435.
11. Hoorfar J., Cook N., Malorny B., Wagner M., De Medici D., Abdulmawjood A., Fach P. Letter to the Editor. J Appl Microbiol 2004, 96:221-222.
12. ISO 16140 Microbiology of food and animal feeding stuff. Protocol for the validation of alternative methods (ISO16140:2003). European Commitee for Standardisation 2003, AFNOR, Paris, France.
13. ISO 17604 Microbiology of food and animal feeding stuffs-caracass sampling for microbiological analysis (ISO 17604:2003). International Organization for Standardization 2003, Geneva, Switzerland.
14. ISO 21528-2 Microbiology of food and animal feeding stuffs. Horizontal methods for the detection and enumeration of Enterobacteriaceae. Colony-Count method, Part 2. International Organisation for Standardisation 2004, Geneva, Switzerland.
15. ISO 6579 Microbiology of food and animal feeding stuffs. Horizonatal method for the detection of Salmonella (ISO 6579:2004). International Organisation for Standardisation 2004, Geneva, Switzerland.
16. Josefsen M.H., Krause M., Hansen F., Hoorfar J. Optimization of a 12-hour TaqMan PCR-based method for detection of Salmonella bacteria in meat. Appl Environ Microbiol 2007, 73:3040-3048.
17. Lofstrom C., Krause M., Josefsen M.H., Hansen F., Hoorfar J. Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella. BMC Microbiol 2009, 9:85.
18. Maciorowski K.G., Pillai S.D., Jones F.T., Ricke S.C. Polymerase chain reaction detection of foodborne Salmonella spp. in animal feeds. Crit Rev Microbiol 2005, 31:45-53.
19. Malorny B., Hoorfar J., Bunge C., Helmuth R. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an International Standard. Appl Environ Microbiol 2003, 69:290-296.
20. Malorny B., Paccassoni E., Fach P., Bunge C., Martin A., Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl Environ Microbiol 2004, 70:7046-7052.
21. McCabe E.M., Burgess C.M., O'Regan E., McGuinness S., Barry T., Fanning S., Duffy G. Development and evaluation of DNA and RNA real-time assays for food analysis using the hilA gene of Salmonella enterica subspecies enterica. Food Microbiol. 2010, In Press, Accepted Manuscript.
22. McGuinness S., McCabe E., O'Regan E., Dolan A., Duffy G., Burgess C., Fanning S., Barry T., O'Grady J. Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat. Meat Sci 2009, 83:555-562.
23. Myint M.S., Johnson Y.J., Tablante N.L., Heckert R.A. The effect of pre-enrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture. Food Microbiol 2006, 23:599-604.
24. Nam H.M., Srinivasan V., Gillespie B.E., Murinda S.E., Oliver S.P. Application of SYBR green real-time PCR assay for specific detection of Salmonella spp. in dairy farm environmental samples. Int J Food Microbiol 2005, 102:161-171.
25. O'Regan E., McCabe E., Burgess C., McGuinness S., Barry T., Duffy G., Whyte P., Fanning S. Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiol 2008, 8:156.
26. Pathmanathan S.G., Cardona-Castro N., Sanchez-Jimenez M.M., Correa-Ochoa M.M., Puthucheary S.D., Thong K.L. Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene. J Med Microbiol 2003, 52:773-776.
27. Perelle S., Dilasser F., Malorny B., Grout J., Hoorfar J., Fach P. Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples. Mol Cell Probes 2004, 18:409-420.
28. Prendergast D.M., Duggan S.J., Gonzales-Barron U., Fanning S., Butler F., Cormican M., Duffy G. Prevalence, numbers and characteristics of Salmonella spp. on Irish retail pork. Int J Food Microbiol 2009, 131:233-239.
29. Rodriguez-Lazaro D., Jofre A., Aymerich T., Garriga M., Pla M. Rapid quantitative detection of, Listeria monocytogenes in salmon products: evaluation of pre-real-time PCR strategies. J Food Prot 2005, 68:1467-1471.
30. Sachs L. Applied statistics: a handbook of techniques 1984, Springer, Heidelberg, Germany. second ed.
31. Tirado C., Schmidt K. WHO surveillance programme for control of foodborne infections and intoxications: preliminary results and trends across greater Europe. World Health Organization. J Infect 2001, 43:80-84.
32. Wegener H.C., Hald T., Lo Fo Wong D., Madsen M., Korsgaard H., Bager F., Gerner-Smidt P., Molbak K. Salmonella control programs in Denmark. Emerg Infect Dis 2003, 9:774-780.
33. http://www.afnor-validation.com/ accessed the 21st of September 2010.
34. http://www.microval.org accessed the 21st of September 2010.