A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220

Protein Expression and Purification

Volumn 76, Issue 1, 2011, Pages 109-114

  Yang, Tao ^a   Yang, Lijun ^a   Chai, Weiran ^b   Li, Renke ^a   Xie, Jun ^a   Niu, Bo ^a,c  

^a SHANXI MEDICAL UNIVERSITY   (China)

^b SHANGHAI JIAO TONG UNIVERSITY   (China)

^c CAPITAL INSTITUTE OF PEDIATRICS   (China)

Author keywords

E. coli; Inclusion bodies; Phage display; ScFv; TNF

Indexed keywords

PEPTIDE LIBRARY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BIOSYNTHESIS; ESCHERICHIA COLI; FEMALE; GENE VECTOR; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOUSE;

ANIMALS; CLONING, MOLECULAR; ESCHERICHIA COLI; FEMALE; GENETIC VECTORS; MICE; MICE, INBRED BALB C; PEPTIDE LIBRARY; SINGLE-CHAIN ANTIBODIES; TUMOR NECROSIS FACTOR-ALPHA;

ESCHERICHIA COLI;

EID: 78650174605     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.10.006     Document Type: Article

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