Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent - Application to a human single-chain Fv fragment

Journal of Immunological Methods

Volumn 219, Issue 1-2, 1998, Pages 119-129

  Tsumoto, Kouhei ^a   Shinoki, Katsutoshi ^a   Kondo, Hidemasa ^a   Uchikawa, Makoto ^b   Juji, Takeo ^b   Kumagai, Izumi ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b JAPANESE RED CROSS MEDICAL CENTER   (Japan)

Author keywords

Glutathione; Human antibody; Inclusion body; Refolding; ScFv

Indexed keywords

ARGININE; IMMUNOGLOBULIN FRAGMENT; MONOCLONAL ANTIBODY; OXIDIZING AGENT; REAGENT; RHESUS D ANTIGEN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ARTICLE; BLOOD GROUP RHESUS SYSTEM; CELL INCLUSION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING;

ANIMALS; ANTIBODIES, MONOCLONAL; ARGININE; DISULFIDES; ESCHERICHIA COLI; GLUTATHIONE; GUANIDINE; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; INCLUSION BODIES; MICE; MURAMIDASE; OXIDANTS; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; REDUCING AGENTS; RH-HR BLOOD-GROUP SYSTEM; SOLUBILITY;

ESCHERICHIA COLI;

EID: 0032191701     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00127-6     Document Type: Article

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