Prokaryotic expression, purification and functional characterization of human FHL3

Biotechnology Letters

Volumn 31, Issue 10, 2009, Pages 1499-1504

  Huang, Xin ^a   Wang, Jinfeng ^a   Xia, Wenrong ^a   Zou, Minji ^a   Xu, Tao ^a   Jin, Zhe ^a   Cai, Xin ^a   Wang, Yuanyuan ^a   Wang, Jiaxi ^a   Xu, Donggang ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

Author keywords

Cell proliferation; Four and a half LIM domain protein 3; Prokaryotic expression; Purification

Indexed keywords

ANGIOGENIN; CDNA LIBRARIES; CYTOSKELETONS; E. COLI; FOUR AND A HALF LIM DOMAIN PROTEIN 3; FUNCTIONAL CHARACTERIZATION; HEPG2 CELLS; INCLUSION BODIES; LIM DOMAIN; MTT ASSAYS; MYOGENESIS; POLYCLONAL ANTIBODY; PROKARYOTIC EXPRESSION; RECOMBINANT PLASMID; RECOMBINANT PROTEIN; SDS-PAGE ANALYSIS; WESTERN-BLOT ANALYSIS;

CELL GROWTH; CELL MEMBRANES; CELL PROLIFERATION; DIALYSIS; DIFFUSERS (OPTICAL); ESCHERICHIA COLI; GROWTH KINETICS; PROTEINS;

PURIFICATION;

COMPLEMENTARY DNA; FHL3 PROTEIN, HUMAN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE;

ARTICLE; CELL INCLUSION; CELL LINE; CELL PROLIFERATION; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIVER CELL; METABOLISM; MOLECULAR CLONING; SPLEEN;

CELL LINE; CELL PROLIFERATION; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; HEPATOCYTES; HUMANS; INCLUSION BODIES; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; RECOMBINANT PROTEINS; SPLEEN; TETRAZOLIUM SALTS; THIAZOLES;

ESCHERICHIA COLI; PROKARYOTA;

EID: 70350088047     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0054-x     Document Type: Article

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