Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coil

Protein Expression and Purification

Volumn 18, Issue 2, 2000, Pages 182-192

  Patra, Ashok K ^a   Mukhopadhyay, R ^a   Mukhija, R ^a   Krishnan, Anuja ^a   Garg, L C ^a   Panda, Amulya K ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Bioactivity; Escherichia coli; Inclusion bodies; Purification; Recombinant human growth hormone

Indexed keywords

BUFFER; CELL INCLUSION; FED BATCH FERMENTATION; HUMAN GROWTH HORMONE; HYDROPHOBICITY; ION EXCHANGE; LYMPHOMA CELL LINE; PH; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SOLUBILITY; SOLUBILIZATION; UREA;

ESCHERICHIA; ESCHERICHIA COLI;

EID: 0034105491     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1179     Document Type: Article

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