Validation of efficient high-throughput plasmid and siRNA transfection of human monocyte-derived dendritic cells without cell maturation

Journal of Immunological Methods

Volumn 363, Issue 1, 2010, Pages 21-28

  Bowles, Robert ^a   Patil, Sonali ^a   Pincas, Hanna ^a   Sealfon, Stuart C ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Dendritic cells; Green fluorescent protein; High throughput; Interferon signaling; Nucleofection; SiRNA

Indexed keywords

CD86 ANTIGEN; GREEN FLUORESCENT PROTEIN; INTERFERON; RETINOIC ACID INDUCIBLE PROTEIN I; SMALL INTERFERING RNA;

ARTICLE; CELL MATURATION; CELL VIABILITY; CONTROLLED STUDY; DENDRITIC CELL; DNA TRANSFECTION; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; SIGNAL TRANSDUCTION; VALIDATION PROCESS;

ANTIGENS, CD86; DEAD-BOX RNA HELICASES; DENDRITIC CELLS; FLOW CYTOMETRY; GENE EXPRESSION; GENE KNOCKDOWN TECHNIQUES; GREEN FLUORESCENT PROTEINS; HISTOCOMPATIBILITY ANTIGENS CLASS II; HUMANS; INTERFERON-BETA; MONOCYTES; NEWCASTLE DISEASE VIRUS; PLASMIDS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

NEWCASTLE DISEASE VIRUS;

EID: 78649700129     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.09.028     Document Type: Article

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