CHO-K1 host cells adapted to growth in glutamine-free medium by FACS-assisted evolution

Biotechnology Journal

Volumn 5, Issue 10, 2010, Pages 1090-1097

  Bort, Juan A Hernández ^a   Stern, Beate ^b   Borth, Nicole ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b Uni Research AS   (Norway)

Author keywords

Ammonia; Assisted evolution; Cell sorting; CHO K1 cells; Glutamine

Indexed keywords

ALTERNATIVE ENERGY SOURCE; ASPARTATES; ASSISTED EVOLUTION; BIOPHARMACEUTICALS; CELL CONCENTRATIONS; CELL LINES; CELL SORTING; CELLULAR PROPERTIES; CHO-K1 CELLS; GLUTAMINE; HIGH CONCENTRATION; HIGH DENSITY; HOST CELLS; NEGATIVE IMPACTS; OPTIMISATIONS; PROTEIN GLYCOSYLATION; PROTEIN YIELD; RAPID EVOLUTION; RECOMBINANT CELLS; VIABLE CELLS;

AMINO ACIDS; AMMONIA; CELL CULTURE; CONCENTRATION (PROCESS); GLUCOSE;

CELLS;

AMINO ACID; AMMONIA; GLUTAMINE; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; LACTIC ACID;

AMINO ACID ANALYSIS; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL GROWTH; CELL METABOLISM; CELL SORTER; CELL SURVIVAL; CELL VIABILITY; CHO CELL; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; ENERGY RESOURCE; ENZYME LINKED IMMUNOSORBENT ASSAY; EVOLUTIONARY ADAPTATION; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC TRANSFECTION; HOST CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL;

ANIMALS; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; FLOW CYTOMETRY; GLUTAMINE;

EID: 78349269949     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000095     Document Type: Article

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