Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit

Plant Physiology

Volumn 140, Issue 1, 2006, Pages 3-11

  Orzaez, Diego ^a   Mirabel, Sophie ^a   Wieland, Willemien H ^b   Granell, Antonio ^a  

^a Ciudad Politécnica de la Innovación   (Spain)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIOSYNTHESIS; BIOTECHNOLOGY; FLUORESCENCE; GENES; INFILTRATION; PROTEINS;

GENE SILENCING; HEAT SHOCK; PERICARP; TRANSGENE EXPRESSION;

FRUITS;

ANTIBODIES; BIOSYNTHESIS; BIOTECHNOLOGY; FLUORESCENCE; GENES; INFILTRATION; PROTEINS; TOMATOES;

AGROBACTERIUM; ARABIDOPSIS; ARABIDOPSIS THALIANA; CAULIFLOWER MOSAIC VIRUS; LYCOPERSICON ESCULENTUM; RHIZOBIUM; SOLANUM;

BACTERIAL PROTEIN; BETA GLUCURONIDASE; HETEROPHILE ANTIBODY; OXIDOREDUCTASE; PHOTOPROTEIN; PHYTOENE DEHYDROGENASE; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; CAULIFLOWER MOSAIC VIRUS; CONFOCAL MICROSCOPY; EVALUATION; FRUIT; GENE SILENCING; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HISTOLOGY; INJECTION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PLASMID; REPORTER GENE; RHIZOBIUM; TOMATO; TRANSGENE; TRANSGENIC PLANT;

ANTIBODIES, HETEROPHILE; BACTERIAL PROTEINS; BIOTECHNOLOGY; CAULIMOVIRUS; FRUIT; GENE SILENCING; GENES, REPORTER; GLUCURONIDASE; INJECTIONS; LUMINESCENT PROTEINS; LYCOPERSICON ESCULENTUM; MICROSCOPY, CONFOCAL; OXIDOREDUCTASES; PLANTS, GENETICALLY MODIFIED; PLASMIDS; RHIZOBIUM; TRANSFORMATION, GENETIC; TRANSGENES;

EID: 33645824134     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.105.068221     Document Type: Article

References (28)

1. Aparicio F, Thomas CL, Lederer C, Niu Y, Wang DW, Maule AJ (2005) Virus induction of heat shock protein 70 reflects a general response to protein accumulation in the plant cytosol. Plant Physiol 138: 529-536
2. Bentley KJ, Gewert R, Harris WJ (1998) Differential efficiency of expression of humanized antibodies in transient transfected mammalian cells. Hybridoma 17: 559-567
3. Chen JC, Jiang CZ, Gookin TE, Hunter DA, Clark DG, Reid MS (2004) Chalcone synthase as a reporter in virus-induced gene silencing studies of flower senescence. Plant Mol Biol 55: 521-530
4. Corthesy B (2002) Recombinant immunoglobulin A: powerful tools for fundamental and applied research. Trends Biotechnol 20: 65-71
5. D'Aoust MA, Lerouge P, Busse U (2004) Efficient and reliable production of pharmaceuticals in alfalfa. In R Fischer, S Schillberg, eds, Molecular Farming. Wiley-VCH Verlag GmbH & Co., Weinheim, Germany
6. Fraser PD, Pinto MES, Holloway DE, Bramley PM (2000) Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids. Plant J 24: 551-558
7. Fray RG, Grierson D (1993) Identification and genetic-analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression. Plant Mol Biol 22: 589-602
8. Fu D-Q, Zhu B-Z, Zhu H-L, Jiang W-B, Luo Y-B (2005) Virus-induced gene silencing in tomato fruits. Plant J 31: 299-308
9. Goodin MM, Dietzgen RG, Schichnes D, Ruzin S, Jackson AO (2002) pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. Plant J 31: 375-383
10. Groot SPC, Karssen CM (1992) Dormancy and germination of abscisic acid-deficient tomato seeds: studies with the sitiens mutant. Plant Physiol 99: 952-958
11. Jani D, Meena LS, Rizwan-ul-Haq QM, Singh Y, Sharma AK, Tyagi AK (2002) Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic Res 11: 447-454
12. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387-405
13. Kapila J, DeRycke R, VanMontagu M, Angenon G (1997) An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci 122: 101-108
14. Liu YL, Schiff M, Dinesh-Kumar SP (2002) Virus-induced gene silencing in tomato. Plant J 31: 777-786
15. Ma Y, Lin SQ, Gao Y, Li M, Luo WX, Zhang J, Xia NS (2003) Expression of ORF2 partial gene of hepatitis E virus in tomatoes and immunoactivity of expression products. World J Gastroenterol 9: 2211-2215
16. Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol 23: 718-723
17. Ratcliff F, Martin-Hernandez AM, Baulcombe DC (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25: 237-245
18. Rodriguez-Concepcion M, Ahumada I, Diez-Juez E, Sauret-Gueto S, Lois LM, Gallego F, Carretero-Paulet L, Campos N, Boronat A (2001) 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening. Plant J 27: 213-222
19. Sandhu JS, Krasnyanski SF, Domier LL, Korban SS, Osadjan MD, Buetow DE (2000) Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response. Transgenic Res 9: 127-135
20. Sharp JM, Doran PM (2001) Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol Bioeng 73: 338-346
21. Spolaore S, Trainotti L, Casadoro G (2001) A simple protocol for transient gene expression in ripe fleshy fruit mediated by Agrobacterium. J Exp Bot 52: 845-850
22. Sung DY, Vierling E, Guy CL (2001) Comprehensive expression profile analysis of the Arabidopsis hsp70 gene family. Plant Physiol 126: 789-800
23. Walmsley AM, Alvarez ML, Jin Y, Kirk DD, Lee SM, Pinkhasov J, Rigano MM, Arntzen CJ, Mason HS (2003) Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato. Plant Cell Rep 21: 1020-1026
24. Walmsley AM, Arntzen CJ (2003) Plant cell factories and mucosal vaccines. Curr Opin Biotechnol 14: 145-150
25. Wieland W (2004) From phage display to plant expression: fulfilling prerequisites for chicken oral immunotherapy against coccidiosis. PhD thesis. Wageningen University, Wageningen, The Netherlands
26. Wieland W, Orzaez D, Lammers A, Schots A (2006) Display and selection of chicken IgA phage fragments. Vet Immunol Immunopathol doi/10.1016/j.vetimm.2005. 09.012 (in press)
27. Wroblewski T, Tomczak A, Michelmore R (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol J 3: 259-273
28. Yang YN, Li RG, Qi M (2000) In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. Plant J 22: 543-551