Application of a PEG/salt aqueous two-phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract

Biotechnology Journal

Volumn 4, Issue 9, 2009, Pages 1320-1327

  Platis, Dimitris ^a   Labrou, Nikolaos E ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

Aqueous two phase system; Downstream processing; Molecular farming; Therapeutic monoclonal antibody; Transgenic plants

Indexed keywords

AQUEOUS TWO-PHASE SYSTEM; DOWNSTREAM PROCESSING; MOLECULAR FARMING; THERAPEUTIC MONOCLONAL ANTIBODY; TRANSGENIC PLANTS;

ALLERGIES; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PURIFICATION; SURFACE PLASMON RESONANCE; TOBACCO;

PLANTS (BOTANY);

MACROGOL; MONOCLONAL ANTIBODY 2G12; MONOCLONAL ANTIBODY 4E10; PROTEIN A; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; AQUEOUS TWO PHASE PARTITION SYSTEM; ARTICLE; NONHUMAN; PHASE PARTITIONING; PHASE SEPARATION; PRIORITY JOURNAL; PURIFICATION; SEPARATION TECHNIQUE; TOBACCO;

ANTIBODIES, MONOCLONAL; CHEMICAL FRACTIONATION; PHASE TRANSITION; PHOSPHATES; PLANT EXTRACTS; PLANTS, GENETICALLY MODIFIED; POLYETHYLENE GLYCOLS; PROTEIN ENGINEERING; TOBACCO;

NICOTIANA TABACUM;

EID: 70350116092     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800359     Document Type: Article

References (48)

1. Ferreira, I. F., Azevedo, A. M., Rosa, P. A., Aires-Barros, M. R., Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems. J. Chromatogr. A 2008, 1195, 94-100.
2. Johansson, H. O., Magaldi, F. M., Feitosa, E., Pessoa, A., Jr., Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems. J. Chromatogr. A 2008, 1178, 145-153.
3. Pericin, D. M., Madarev-Popovic, S. Z., Radulovic-Popovic, L. M., Optimization of conditions for acid protease partitioning and purification in aqueous two-phase systems using response surface methodology. Biotechnol. Lett. 2008, 31, 43-47.
4. Salgado, J.C., Andrews, B. A., Ortuzar, M. F., Asenjo, J. A., Prediction of the partitioning behavior of proteins in aqueous two-phase systems using only their amino acid composition. J. Chromatogr. A 2008, 1178, 134-144.
5. Albertsson, P. A., Partition of cell particles and macromolecules in polymer two-phase systems. Adv. Protein Chem. 1970, 24, 309-341.
6. Flanagan, J. A., Huddleston, J. G., Lyddiatt, A., Application of aqueous two-phase partition in PEG-phosphate systems. Design and implementation of a prototype process for recovery of bulk protein fractions from waste brewer's yeast. Bioseparation 1991, 2, 43-61.
7. Hustedt, H., Kroner, K. H., Kula, M. R., In: Walter, H., Brooks, D. E., Fisher, D. (Eds.), Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology. Academic Press, New York 1985, p. 529.
8. Kula, M. R., Kroner, K. H., Hustedt, H., Schutte, H., Technical aspects of extractive enzyme purification. Ann. NY Acad. Sci. 1981, 369, 341-354.
9. Dreyer, S., Kragl, U., Ionic liquids for aqueous two-phase extraction and stabilization of enzymes. Biotechnol. Bioeng. 2008, 99, 1416-1424.
10. Andrews, B. A., Schmidt, A. S., Asenjo, J. A., Correlation for the partition behavior of proteins in aqueous two-phase systems: effect of surface hydrophobicity and charge. Biotechnol. Bioeng. 2005, 90, 380-390.
11. Huddleston, J., Veide, A., Kohler, K., Flanagan, J. et al., The molecular basis of partitioning in aqueous two-phase systems. Trends Biotechnol. 1991, 9, 381-388.
12. Rosa, P. A., Azevedo, A. M., Ferreira, I. F., de Vries, J. et al., Affinity partitioning of human antibodies in aqueous two-phase systems. J. Chromatogr. A 2007, 1162, 103-113.
13. Azevedo, A. M., Rosa, P. A., Ferreira, I. F., Aires-Barros, M. R., Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol. 2009, 27, 240-247.
14. Hassan, S., van Dolleweerd, C. J., Ioakeimidis, F., Keshavarz-Moore, E., Ma, J. K., Considerations for extraction of monoclonal antibodies targeted to different subcellular compartments in transgenic tobacco plants. Plant Biotechnol. J. 2008, 6, 733-748.
15. Lienard, D., Sourrouille, C., Gomord, V., Faye, L., Pharming and transgenic plants. Biotechnol. Annu. Rev. 2007, 13, 115-147.
16. Zhang, C., Glatz, C. E., Process engineering strategy for recombinant protein recovery from canola by cation exchange chromatography. Biotechnol. Prog. 1999, 15, 12-18.
17. Wang, C., Soice, N. P., Ramaswamy, S., Gagnon, B. A. et al., Cored anion-exchange chromatography media for antibody flow-through purification. J. Chromatogr. A 2007, 1155, 74-84.
18. Gupta, M. N., Jain, S., Roy, I., Immobilized metal affinity chromatography without chelating ligands: purification of soybean trypsin inhibitor on zinc alginate beads. Biotechnol. Prog. 2002, 18, 78-81.
19. Zhang, C. M., Reslewic, S. A., Glatz, C. E., Suitability of immobilized metal affinity chromatography for protein purification from canola. Biotechnol. Bioeng. 2000, 68, 52-58.
20. Menkhaus, T. J., Eriksson, S. U., Whitson, P. B., Glatz, C. E., Host selection as a downstream strategy: polyelectrolyte precipitation of beta-glucuronidase from plant extracts. Biotechnol. Bioeng. 2002, 77, 148-154.
21. Ramessar, K., Rademacher,T., Sack, M., Stadlmann, J. et al., Cost-effective production of a vaginal protein microbicide to prevent HIV transmission. Proc. Natl. Acad. Sci. USA 2008, 105, 3727-3732.
22. Platis, D., Sotriffer, C. A., Clonis,Y., Labrou, N. E., Lock-andkey motif as a concept for designing affinity adsorbents for protein purification. J. Chromatogr. A 2006, 1128, 138-151.
23. Platis, D., Labrou, N. E., Affinity chromatography for the purification of therapeutic proteins from transgenic maize using immobilized histamine. J. Sep. Sci. 2008, 31, 636-645.
24. Balasubramaniam, D., Wilkinson, C., Van Cott, K., Zhang, C., Tobacco protein separation by aqueous two-phase extraction. J. Chromatogr. A 2003, 989, 119-129.
25. Platis, D., Labrou, N. E., Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract. J. Chromatogr. A 2006, 1128, 114-124.
26. Platis, D., Drossard, J., Fischer, R., Ma, J.-K. C., Labrou, N. E., New downstream processing strategy for the purification of monoclonal antibodies from transgenic tobacco plants. J. Chromatogr. A 2008, 1211, 80-89.
27. Andrews, B. A., Nielsen, S., Asenjo, J.A., Partitioning and purification of monoclonal antibodies in aqueous two-phase systems. Bioseparation 1996, 6, 303-313.
28. Wingren, C., Michaelsen, T. E., Magnusson, C. G., Hansson, U. B., Comparison of surface properties of human IgA, IgE, IgG and IgM antibodies with identical and different specificities. Scand. J. Immunol. 1996, 44, 430-436.
29. Li, C., Bai, J., Li, W., Cai, Z., Ouyang, F., Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs. Biotechnol. Prog. 2001, 17, 366-368.
30. Rosa, P. A., Azevedo, A. M., Aires-Barros, M. R., Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies. J. Chromatogr. A 2007, 1141, 50-60.
31. Jervis, L., Pierpoint, W. S., Purification technologies for plant proteins. J. Biotechnol. 1989, 11, 161-198.
32. Loomis, W.D., Battaile, J., Plant phenolic compounds and the isolation of plant enzymes. Phytochemistry 1966, 5, 423-438.
33. Loomis, W. D., Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 1974, 31, 528-544.
34. Smith, D. M., Montgomery, M.W., Improved methods for the extraction of polyphenol oxidase from d'anjou pears. Phytochemistry 1985, 24, 901-904.
35. Hagerman, A. E., Butler, L. G., The specificity of proanthocyanidin protein interactions. J. Biol. Chem. 1981, 256, 4494-4497.
36. Spencer, C. M., Cai, Y., Martin, R., Gaffney, S. H. et al., Polyphenol complexation - some thoughts and observations. Phytochemistry 1988, 27, 2397-2409.
37. Hood, E. E., Kusnadi, A., Nikolov, Z., Howard, J. A., Molecular farming of industrial proteins from transgenic maize. Adv. Exp. Med. Biol. 1999, 464, 127-147.
38. Woodard, S. L., Mayor, J. M., Bailey, M. R., Barker, D. K. et al., Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotechnol. Appl. Biochem. 2003, 38, 123-130.
39. Suzuki, Y. A., Kelleher, S. L., Yalda, D., Wu, L. et al., Expression, characterization, and biologic activity of recombinant human lactoferrin in rice. J. Pediatr. Gastroenterol. Nutr. 2003, 36, 190-199.
40. Yang, D., Guo, F., Liu, B., Huang, N., Watkins, S. C., Expression and localization of human lysozyme in the endosperm of transgenic rice. Planta 2003, 216, 597-603.
41. Arlen, P.A., Falconer, R., Cherukumilli, S., Cole, A. et al., Field production and functional evaluation of chloroplast-derived interferon-alpha2b. Plant Biotechnol. J. 2007, 5, 511-525.
42. Hart, R.A., Lester, P. M., Reifsnyder, D. H., Ogez, J. R., Builder, S. E., Large scale, in situ isolation of periplasmic IGF-I from E. coli. Biotechnology (NY) 1994, 12, 1113-1117.
43. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
44. Snyder, S. M., Cole, K. D., Szlag, D. C., Phase compositions, viscosities and densities of aqueous two-phase systems composed of polyethylene glycol and various salts at 25°C. J. Chem. Eng. Data 1992, 37, 268-274.
45. Zaslavsky, B. Y., Aqueous two-phase partitioning. Physical Chemistry and Bioanalytical Applications. Marcel Dekker, New York 1995.
46. Cisneros, M., Benavides, J., Brenes, C. H., Rito-Palomares, M., Recovery in aqueous two-phase systems of lutein produced by the green microalga Chlorella protothecoides. J. Chromatogr.B Analyt. Technol. Biomed. Life Sci. 2004, 807, 105-110.
47. Srinivas, N. D., Barhate, R. S., Raghavarao, K. S.M. S., Aqueous two-phase extraction in combination with ultrafiltration for downstream processing of Ipomoea peroxidase. J. Food Eng. 2002, 54, 1-6.
48. Platis, D., Maltezos, A., Ma, J., Labrou, N.E., Combinatorial de novo design and application of a biomimetic affinity ligand for the purification of human anti-HIV mAb 4E10 from transgenic tobacco. J. Mol. Recognit. 2009, in press, http://dx.doi.org/10.1002/jmr.954.