A modified fluorescent intercalator displacement assay for RNA ligand discovery

Analytical Biochemistry

Volumn 408, Issue 2, 2011, Pages 269-276

  Asare Okai, Papa Nii ^a   Chow, Christine S ^a  

^a WAYNE STATE UNIVERSITY   (United States)

Author keywords

Drug discovery; ESI MS; FID; Ribosome; RNA

Indexed keywords

BINDING ENERGY; DRUG PRODUCTS; ELECTRODEPOSITION; ELECTROSPRAY IONIZATION; FLUORESCENCE; MASS SPECTROMETRY; RNA;

DRUG DISCOVERY; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ESI-MS; FLUORESCENCE INTENSITIES; FLUORESCENT INDICATORS; RELATIVE BINDING AFFINITY; RIBOSOME; TRANS-ACTIVATION RESPONSE;

LIGANDS;

3 METHYL 2 [[1 (3 (TRIMETHYLAMMONIO)PROPYL] 4 QUINOLINYLIDENE]METHYL)BENZOTHIAZOLIUM; AMINOGLYCOSIDE DERIVATIVE; AMMONIUM ACETATE; BACTERIAL RNA; CHLORAMPHENICOL; FLUORESCENT DYE; LIGAND; PAROMOMYCIN; PEPTIDE DERIVATIVE; RNA 16S; RNA 23S; SHORT HAIRPIN RNA; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG; VIRUS RNA;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FLUORESCENT INTERCALATOR DISPLACEMENT ASSAY; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; RNA ANALYSIS; RNA BINDING; RNA STRUCTURE;

EID: 78149411111     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.09.020     Document Type: Article

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