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O. Lenzen E. Defrancq J. F. Constant S. Schumm D. Garcia-Fresnadillo C. Moucheron P. Dumy A. Kirsch-De Mesmaeker JBIC, J. Biol. Inorg. Chem. 2004 9 100 108
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The term "seppuku" refers to the behaviour, in the antic Japanese society, of someone who prefers to suicide rather than to accomplish an immoral order
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77957662460
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One should note that metal-ODN conjugates that can bind DNA according to a mechanism which do not involve a ligand exchange process on the metal centre, thus leading to kinetically stable photo-adducts, are very rare
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For a review related to oligonucleotide conjugation via oxime bond formation see:
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2+ derivatives for CT-DNA, thus a similar loading of the 17-mer duplex by these complexes is likely to occur at the same Ru/ODN ratio By monitoring the changes of luminescence of the complexes upon addition of excess CT-DNA (P/Ru = 100), we observed that a thermodynamic equilibrium was reached within 1-2 min, and with no detectable difference between the derivatised complexes (see ESI). This was surprising since it was expected that the presence of the linker, with a bulky Boc protecting group on the intercalating dppz ligand, would generate a steric hindrance responsible for a slow threading between the base pairs. The data show however that the linker does not affect the intercalation
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