CYP2E1-dependent toxicity and oxidative stress in HepG2 cells

Free Radical Biology and Medicine

Volumn 31, Issue 12, 2001, Pages 1539-1543

  Cederbaum, Arthur I ^a   Wu, Defeng ^a   Mari, Montserrat ^a   Bai, Jingxiang ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

CYP2E1; Ethanol toxicity; Free radicals; Glutathione; Lipid peroxidation; Oxidative stress

Indexed keywords

ALCOHOL; ANTIOXIDANT; ARACHIDONIC ACID; CATALASE; CYTOCHROME P450 2E1; ENZYME INHIBITOR; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; IRON; UNSATURATED FATTY ACID;

ALCOHOL INTOXICATION; ALCOHOL LIVER CIRRHOSIS; APOPTOSIS; CELL VIABILITY; CYTOTOXICITY; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE CONTROL; HEPATOMA CELL; HUMAN; LIPID PEROXIDATION; MEMBRANE POTENTIAL; MITOCHONDRION; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVIEW;

EID: 0035894132     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(01)00743-2     Document Type: Article

References (38)

1. Role of human cytochrome P450 IIE1 in the oxidation of many low molecular weight cancer suspects
2. Cytochrome P450 IIE1: Roles in nitrosamine metabolism and mechanism of regulation
3. Oxidative and reductive metabolism by cytochrome P4502E1
4. Lipid peroxidation in ethanol poisoning: A critical reconsideration
5. Microsomal generation of reactive oxygen species and their possible role in alcohol hepatoxicity
6. Ethanol toxicity and oxidative stress
7. Rat liver microsomal NADPH-supported oxidase activity and lipid peroxidation dependent on ethanol-inducible cytochrome P450
8. DNA strand cleavage as a sensitive assay for the production of hydroxyl radical by microsomes: Roles of CYP2E1 in the increased activity after ethanol treatment
9. Role of cytochrome P4502E1 in ethanol-, carbon tetrachloride-, and iron-dependent microsomal lipid peroxidation
10. Fish oil, alcohol, and liver pathology: Role of cytochrome P4502E1
11. Markedly enhanced cytochrome P4502E1 induction and lipid peroxidation is associated with severe liver injury in fish oil-ethanol-fed rats
12. Stable transfection of human cytochrome P4502E1 in HepG2 cells: Characterization of catalytic activities and production of reactive oxygen intermediates
13. Cytotoxicity and apoptosis produced by cytochrome P450 2E1 in HepG2 cells
14. Cytotoxicity of acetaminophen in human cytochrome P4502E1-transfected HepG2 cells
15. Inactivation and degradation of human cytochrome P4502E1 by CC14 in a transfected HepG2 cell line
16. Ethanol toxicity to transfected HepG2 cells expressing human ctyochrome P4502E1
17. Cytotoxicity and apoptosis produced by arachidonic acid in HepG2 cells overexpressing human cytochrome P4502E1
18. Mitochondrial dysfunction in the pathogenesis of necrotic and apoptotic cell death
19. Mitochondrial implication in accidental and programmed cell death: Apoptosis and necrosis
20. Ethanol-induced apoptosis to HepG2 cell lines expressing human cytochrome P4502E1
21. Experimental liver cirrhosis induced by alcohol and iron
22. Alcohol mediates increases in hepatic and serum nonheme iron stores in a rat model for alcohol-induced liver injury
23. Oxygen toxicity, oxygen radical, transition metals and disease
24. Oxidative stress and cytoxicity induced by ferric-nitrilotriacetate in HepG2 cells expressing CYP2E1
25. Removal of glutathione produces apoptosis and necrosis in HepG2 cells overexpressing CYP2E1
26. Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 against oxidative injury
27. Adenovirus-mediated overexpression of catalase in the cytosolic or mitochondrial compartment protects against cytochrome P4502E1-dependent toxicity in HepG2 cells
28. CYP2E1 overexpression in HepG2 cells induces glutathione synthesis by transcriptional activation of y-glutamylcysteine synthetase
29. Induction of catalase, alpha and microsomal glutathione S-transferase in CYP2E1-overexpressing HepG2 cells and protection against short-term oxidative stress
30. Ethanol potentiates tumor necrosis factor-α cytotoxicity in hepatoma cells and primary rat hepatocytes by promoting induction of the mitochondrial permeability transition
31. Differential role of ethanol and acetaldehyde in the induction of oxidative stress in HepG2 cells: Effect of transcription factors AP-1 and NF-kB
32. Additive activation of hepatic NF-kB by ethanol and hepatitis B protein X (HBX) or HCV core protein by PKC-, PKB/AKT-and NIK-(KK-)kB-dependent mechanisms
33. Gadolinium chloride blocks alcohol-dependent liver toxicity in rats treated chronically with intragastric alcohol despite the induction of CYP2E1
34. CYP2E1 is not involved in early alcohol-induced liver injury
35. Gadolinium chloride reduces cytochrome P450: Relevance to chemical-induced hepatotoxicity
36. Kupffer cell inactivation alleviates ethanol-induced steatosis and CYP2E1 induction but not inflammatory responses in rat liver
37. CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis
38. The effect of ethanol-induced cytochrome P4502E1 on the inhibition of proteasome activity by alcohol